Factory source Nanopore Sequencing - LncRNA

Factory source Nanopore Sequencing - LncRNA – Biomarker

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Factory source Nanopore Sequencing - LncRNA – Biomarker Detail:

Bioinformatics

Raw data quality control

Alignment with reference genome

Transcript assembly

Correlation analysis

Known lncRNA identification

Coding potential of candidate lncRNA

Regulatory network: lncRNA-mRNA

lncRNA expression quantification

Differentially expressed lncRNA (DE-lncRNA)

PCA

WGCNA

LncRNA target gene prediction

GO and KEGG enrichment on DE-lncRNA target genes

Novel lncRNA

Alternative splicing prediction

mRNA expression analysis

Comparative analysis lncRNA vs. mRNA

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Gene Expression Profiling