2021 New Style Metagenome Sequencing - Nanopore Full-length transcriptomics

2021 New Style Metagenome Sequencing - Nanopore Full-length transcriptomics – Biomarker

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2021 New Style Metagenome Sequencing - Nanopore Full-length transcriptomics – Biomarker Detail:

Bioinformatics

Raw data quality control

Full-length transcript assessment

Transcript de-redundance

Gene family

Gene structure optimization

Novel transcript prediction

Alternative splicing

Function annotation

Fusion transcript identification

lncRNA prediction

SSR analysis

CDS prediction

Transcription factor prediction

Alternative polyadenylation(APA)

Function enrichment

Differential alternative splicing analysis

Differential fusion gene analysis

Reproducibility test

Expression quantification on genes/transcriptS

Differentially expressed genes(DEGs) and transcripts(DETs)

Hierarchical clustering

Function annotation

Protein-protein interaction network

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