Raw data quality control
Common and unique sequence among samples
Small RNA classification
Alignment with reference genome (+/- strands)
Genome-wide smallRNA distribution genes
Known miRNA identification
Small RNA annotation (miRBASE, RepBASE, Rfam, SILVA, GtRNAdb, etc.)
miRNA base perference
miRNA base editing
miRNA family
miRNA quantification
Correlation analysis
miRNA differential expression analysis
Hierarchical clustering on DE-miRNAs
miRNA target gene prediction
Function annotation on DE-miRNA target genes
GO and KEGG enrichment on DE-miRNA target genes