Ukulandelana okuphelele kwe-genome bisulfite
Izinzuzo Zesevisi
● Inkundla ephelele: hlinzeka ngesevisi enhle kakhulu yesitobhi esisodwa kusukela ekucutshungulweni kwesampula, ukwakhiwa komtapo wolwazi, ukulandelana kuya ekuhlaziyweni kwe-bioinformatics.
● Ukunemba okuphezulu: Ubuchwepheshe bokucubungula ukuguqulwa kwe-methylation ekhulile bungakwazi ukuhlaziya ngokunembile isimo se-methylation sesisekelo esisodwa se-C.
● Ukufakwa okubanzi: ukutholwa kwamasayithi e-methylation ezingeni le-genome-wide.
● Ukuphindaphinda okuhle: ukucubungula okuzinzile, kulungele ukuhlaziya okuqhathanisayo phakathi kwamasampuli amaningi.
Imininingwane Yesampula
|
Inkundla
|
Funda Ubude
|
Isu Lokulandelanisa
|
| Illumina | PE150 | 30X, iziphindaphindo ezintathu zebhayoloji, ezinereferensi yegenome yekhwalithi ephezulu |
Izidingo Zesampula
|
Uhlobo lwesampula
|
Inani (μg)
|
Conc.(ng/μl)
|
Ivolumu (μl)
|
Ubumsulwa
|
| gDNA | ≥ 2 | ≥ 20
| ≥ 20
| Ukucekelwa phansi nokungcoliswa okulinganiselwe
|
Ukugeleza Komsebenzi Wesevisi
Ukulethwa kwesampula
Ukukhishwa kwe-DNA
Ukwakhiwa komtapo wolwazi
Ukulandelanisa
Ukuhlaziywa kwedatha
Ukulethwa kwedatha
I-Bioinformatics
1.I-Genome-wide DNA methylation distribution
2.Isichasiselo endaweni ye-CGI ene-methylated kakhulu
