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Ukulandelanisa Kuphela

  • DNA/RNA sequencing -PacBio Sequencer

    Ukulandelana kwe-DNA/RNA -I-PacBio Sequencer

    Inkundla yokulandelana ye-PacBio iyinkundla yokulandelana efunde isikhathi eside, eyaziwa nangokuthi obunye bobuchwepheshe bokulandelana kwesizukulwane sesithathu (TGS).Ubuchwepheshe obuyisisekelo, i-single-molecule real-time(SMRT), inika amandla ukukhiqizwa kokufunda ngamashumi ama-kilo-base ubude.Ngesisekelo se-“Sequencing-by-Synthesis”, ukulungiswa kwe-nucleotide eyodwa kufinyelelwa yi-Zero-mode waveguide(ZMW), lapho kukhanyiswa khona ivolumu elinganiselwe ezansi(indawo yokuhlanganisa i-molecule).Ukwengeza, ukulandelana kwe-SMRT kugwema kakhulu ukuchema okuqondile kokulandelana ohlelweni lwe-NGS, ngokuthi izinyathelo eziningi zokukhulisa i-PCR azidingeki kunqubo yokwakha umtapo wolwazi.

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