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I-Single-nucleus RNA Sequencing
Ukuthuthuka ekuthwebuleni iseli elilodwa kanye nezindlela zokwakha umtapo wolwazi ngamunye okuhlanganiswe nokulandelana komphumela ophezulu kuvumela izifundo zesisho sofuzo ngesisekelo seseli-by-cell.Inika amandla ukuhlaziya okujulile nokuphelele kwesistimu kumaseli ayinkimbinkimbi, lapho igwema khona kakhulu ukufihla ukuhlukahluka kwawo ngokuthatha isilinganiso sawo wonke amaseli.
Kodwa-ke, amanye amangqamuzana awafanele ukwenziwa abe yi-single-cell ukumiswa, ngakho-ke ezinye izindlela zokulungiselela isampula - ukukhishwa kwe-nucleus ezicutshini ziyadingeka, okungukuthi, i-nucleus ikhishwa ngokuqondile kumathishu noma ingqamuzana futhi ilungiselwe ukumiswa kwe-nucleus eyodwa ukuze kusetshenziswe eyodwa- ukulandelana kweseli.
I-BMK ihlinzeka ngesevisi yokulandelana kwe-RNA eyi-10× Genomics ChromiumTM.Le nsizakalo isetshenziswe kakhulu ezifundweni eziphathelene nezifo, njengokuhlukaniswa kwamaseli omzimba, i-tumor heterogeneity, ukuthuthukiswa kwezicubu, njll.
I-Spatial transcriptome chip: 10× Genomics
Ipulatifomu: I-Illumina NovaSeq Platform
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I-BMKMANU S1000 Spatial Transcriptome
I-Spatial transcriptomics imi phambili ekusungulweni kwesayensi, inika amandla abacwaningi ukuthi bahlole amaphethini enkulumo eyinkimbinkimbi yezakhi zofuzo phakathi kwezicubu kuyilapho belondoloza isimo sabo sendawo.Phakathi kwamapulatifomu ahlukahlukene, i-BMKGene ithuthukise i-BMKManu S1000 Spatial Transcriptome Chip, iziqhayisaukulungiswa okuthuthukisiweyokungu-5µM, ifinyelela kububanzi obuncane beselula, futhi inika amandlaizilungiselelo zokulungiswa kwamazinga amaningi.I-chip ye-S1000, equkethe cishe izindawo eziyizigidi ezingu-2, isebenzisa ama-microwell agqitshwe ngobuhlalu obulayishwe ama-probe wokuthwebula anebhakhodi yendawo.Umtapo wezincwadi we-cDNA, ohlanganiswe namabhakhodi wendawo, ulungiswa kusukela ku-chip ye-S1000 bese ulandelana ngokulandelana endaweni ye-Illumina NovaSeq.Inhlanganisela yamasampula anebhakhodi yendawo kanye nama-UMI kuqinisekisa ukunemba nokucaciswa kwedatha ekhiqizwayo.Isibaluli esiyingqayizivele se-chip ye-BMKManu S1000 silele ekusebenziseni kwayo izinto ezihlukahlukene, enikeza izilungiselelo zokuxazulula amaleveli amaningi ezingashunwa kahle kumathishu ahlukene namazinga emininingwane.Lokhu kuzivumelanisa nezimo kubeka i-chip njengenketho evelele yezifundo ezihlukahlukene ze-spatial transcriptomics, iqinisekisa ukuhlangana okunembayo kwendawo okunomsindo omncane.
Besebenzisa i-chip ye-BMKManu S1000 nobunye ubuchwepheshe be-transcriptomics yendawo, abacwaningi bangathola ukuqonda okungcono kwenhlangano yendawo yamangqamuzana kanye nokusebenzisana okuyinkimbinkimbi kwamangqamuzana okwenzeka ngaphakathi kwezicubu, kunikeze ukuqonda okubalulekile kwezinqubo eziyisisekelo zezinqubo zebhayoloji emikhakheni eminingi ehlukahlukene, kufaka phakathi. i-oncology, i-neuroscience, i-development biology, i-immunology kanye nezifundo ze-botanical.
I-Platform: BMKManu S1000 chip kanye ne-Illumina NovaSeq
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I-10x Genomics Visium Spatial Transcriptome
I-Spatial transcriptomics ubuchwepheshe obusezingeni eliphezulu obuvumela abacwaningi ukuthi baphenye amaphethini wokusho izakhi zofuzo ngaphakathi kwezicubu ngenkathi belondoloza umongo wabo wendawo.Inkundla eyodwa enamandla kulesi sizinda yi-10x Genomics Visium ehambisana nokulandelana kwe-Illumina.Umgomo we-10X Visium ulele ku-chip ekhethekile enendawo ekhethiwe yokuthwebula lapho kubekwa khona izingxenye zezicubu.Le ndawo yokuthwebula iqukethe izindawo ezinebhakhodi, ngalinye lihambisana nendawo ehlukile ngaphakathi kwethishu.Ama-molecule e-RNA athunjiwe asuka kusicubu abe eselebula ngezihlonzi eziyingqayizivele zamangqamuzana (UMIs) phakathi nenqubo yokuhlehla yokubhala.Lezi zindawo ezinebhakhodi kanye nama-UMI anika amandla ukumepha indawo okunembayo kanye nomthamo wenkulumo yofuzo ekulungisweni kweseli elilodwa.Inhlanganisela yamasampula anebhakhodi yendawo kanye nama-UMI kuqinisekisa ukunemba nokucaciswa kwedatha ekhiqizwayo.Ngokusebenzisa lobu buchwepheshe be-Spatial Transcriptomics, abacwaningi bangathola ukuqonda okujulile kwenhlangano yendawo yamangqamuzana kanye nokusebenzisana okuyinkimbinkimbi kwamangqamuzana okwenzeka ngaphakathi kwezicubu, okuhlinzeka ngemininingwane ebalulekile ezindleleni eziyisisekelo zezinqubo zebhayoloji emikhakheni eminingi, okubandakanya i-oncology, neuroscience, biology yentuthuko, i-immunology. , kanye nezifundo ze-botanical.
I-Platform: 10X Genomics Visium kanye ne-Illumina NovaSeq
