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BSA
Iplathifomu yokuhlaziywa kwe-Bulked Segregant iqukethe ukuhlaziya okujwayelekile okuyisinyathelo esisodwa kanye nokuhlaziya okuthuthukile ngokulungiselelwa kwepharamitha engokwezifiso.I-BSA yindlela esetshenziswa ukuhlonza ngokushesha izimpawu zofuzo ezihlobene ne-phenotype.Ukuhamba komsebenzi okuyinhloko kwe-BSA kuqukethe: 1. ukukhetha amaqembu amabili abantu abane-phenotypes ephikisana kakhulu;2. ukuhlanganisa i-DNA, i-RNA noma i-SLAF-seq(Ithuthukiswe yi-Biomarker) yabo bonke abantu ukuze bakhe inqwaba ye-DNA;3. ukuhlonza ukulandelana okuhlukile ngokumelene ne-reference genome noma phakathi, 4. ukubikezela ikhandidethi izifunda ezixhunywe nge-ED kanye ne-SNP-index algorithm;5. Ukuhlaziywa kokusebenza kanye nokunothisa izakhi zofuzo ezifundeni zamakhandidethi, njll. Ukumbiwa kwezimayini okuthuthuke kakhulu kudatha okuhlanganisa nokuhlolwa komaka wofuzo kanye nomklamo wokuqala nakho kuyatholakala.
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I-Amplicon (16S/18S/ITS)
Iplathifomu ye-Amplicon (16S/18S/ITS) yathuthukiswa yaba neminyaka yokuhlangenwe nakho ekuhlaziyweni kwephrojekthi ye-microbial diversity, equkethe ukuhlaziya okuyisisekelo okujwayelekile kanye nokuhlaziya komuntu siqu: ukuhlaziya okuyisisekelo kuhlanganisa okuqukethwe kokuhlaziya okujwayelekile kocwaningo lwamanje lwe-microbial, okuqukethwe kokuhlaziya kucebile futhi kubanzi, futhi imiphumela yokuhlaziya yethulwa ngendlela yemibiko yephrojekthi;Okuqukethwe kokuhlaziywa komuntu siqu kuhlukahlukene.Amasampuli angakhethwa futhi amapharamitha angasethwa ngendlela evumelana nezimo ngokuvumelana nombiko wokuhlaziya oyisisekelo kanye nenjongo yocwaningo, ukuze kufezeke izidingo eziqondene nawe.Isistimu yokusebenza yeWindows, elula futhi esheshayo.
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I-Evolutionary Genetics
I-Evolutionary genetics iyisevisi egcwele yokulandelana eyenzelwe ukuhlinzeka ngencazelo ebanzi yolwazi lokuziphendukela kwemvelo lwezinto ezinikeziwe ngokusekelwe ezinhlobonhlobo zofuzo, okuhlanganisa ama-SNP, ama-InDels, ama-SV nama-CNV.Ihlinzeka ngakho konke ukuhlaziya okuyisisekelo okudingekayo ekuchazeni izinguquko zokuziphendukela kwemvelo kanye nezici zofuzo zabantu, njengesakhiwo sabantu, ukuhlukahluka kofuzo, ubudlelwano be-phylogeny, njll. Futhi iqukethe izifundo ngokugeleza kwezakhi zofuzo, okunika amandla ukulinganiswa kobukhulu besibalo esisebenzayo, isikhathi sokuhlukana.
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I-Genomics yokuqhathanisa
I-genomics yokuqhathanisa ngokwezwi nezwi isho ukuqhathanisa ukulandelana okuphelele kwe-genome nezakhiwo zezinhlobo ezahlukene.Lesi sinqumo sihlose ukuveza ukuvela kwezinhlobo zezilwane, ukusebenza kwezakhi zofuzo, indlela yokulawula izakhi zofuzo ezingeni le-genome ngokuhlonza izakhiwo zokulandelana nezici ezilondolozwe noma ezehlukanisa kuzo zonke izinhlobo zezinhlobo ezahlukene.Ucwaningo olujwayelekile lokuqhathanisa lwe-genomics luhlanganisa ukuhlaziya emndenini wezakhi, ukukhula kokuziphendukela kwemvelo, ukuphindaphinda kofuzo lonke, ingcindezi ekhethiwe, njll.
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I-Evolutionary Genetics
Inkundla yokuhlaziya izakhi zofuzo yabantu kanye nokuziphendukela kwemvelo isungulwa ngokusekelwe kokuhlangenwe nakho okukhulu okuqoqwe eqenjini le-BMK R&D iminyaka.Kuyithuluzi elisebenziseka kalula ikakhulukazi kubacwaningi abangafundi kakhulu ku-bioinformatics.Le nkundla yenza kube lula ukuhlaziya okuyisisekelo okuhlobene nezakhi zofuzo okuhlanganisa ukwakhiwa kwesihlahla se-phylogenetic, ukuhlaziywa kokungalingani, ukuhlola ukuhlukahluka kwezakhi zofuzo, ukuhlaziya okukhethiwe, ukuhlaziywa kokuzalana, i-PCA, ukuhlaziywa kwesakhiwo sabantu, njll.
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I-Hi-C based Genome Assembly
I-Hi-C iyindlela edizayinelwe ukuthwebula ukucushwa kwe-chromosome ngokuhlanganisa ukusebenzelana okusekelwe ekuhloleni kanye nokulandelana komphumela ophezulu.Ukuqina kwalokhu kusebenzisana kukholakala ukuthi kuhlotshaniswa kabi nebanga elingokomzimba kuma-chromosome.Ngakho-ke, idatha ye-Hi-C ingaqondisa ukuhlanganisa, ukuhleleka nokuqondiswa kokulandelana okuqoqiwe ku-genome esalungiswa futhi kunamathisele lokho enanini elithile lama-chromosome.Lobu buchwepheshe bunika amandla ukuhlangana kweleveli ye-chromosome yofuzo ngaphandle kwemephu yofuzo esekelwe kubantu.Yonke i-genome eyodwa idinga i-Hi-C.
Ipulatifomu: I-Illumina NovaSeq Platform / DNBSEQ
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Ukulandelanisa Kwezitshalo/Isilwane Se-De Novo Genome
De Novoukulandelana kubhekisela ekwakhiweni kofuzo lohlobo lonke kusetshenziswa ubuchwepheshe bokulandelana, isb i-PacBio, i-Nanopore, i-NGS, njll., ingekho i-genome eyireferensi.Ukuthuthukiswa okuphawulekayo kobude bokufunda kobuchwepheshe bokulandelana kwesizukulwane sesithathu kulethe amathuba amasha ekuhlanganiseni ama-genome ayinkimbinkimbi, njengalawo ane-heterozygosity ephezulu, isilinganiso esiphezulu sezifunda eziphindaphindayo, ama-polyploid, njll. Ngobude bokufunda ezingeni lama-kilobase amashumi, lokhu kufundwa kokulandelana kwenza kube lula. ukuxazulula izici eziphindaphindayo, izifunda ezinokuqukethwe kwe-GC okungajwayelekile nezinye izifunda eziyinkimbinkimbi kakhulu.
I-Platform: I-PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq Platform
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Ukulandelana kwe-Human Whole Exome
I-Whole exome sequencing (WES) ithathwa njengesu lokulandelana elingabizi kakhulu lokuhlonza ukuguqulwa okubangela izifo.Nakuba ama-exons athatha kuphela cishe u-1.7% wayo yonke i-genome, imele iphrofayili yengqikithi yemisebenzi yamaprotheni ngokuqondile.Ku-genome yomuntu, kubikwe ukuthi ngaphezu kwama-85% wezinguquko ezihlobene nesifo zenzeka endaweni yokufaka ikhodi yamaprotheni.
I-BMKGENE inikeza izinsiza eziphelele neziguquguqukayo zokulandelanisa i-exome yomuntu ngamasu ahlukene okuthwebula ama-exon atholakalayo ukuze kuhlangatshezwane nezinjongo zocwaningo ezihlukahlukene.
Ipulatifomu: I-Illumina NovaSeq Platform
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I-Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
I-genotyping ephezulu kakhulu, ikakhulukazi kubantu abaningi, iyisinyathelo esibalulekile ezifundweni zenhlangano yofuzo, ehlinzeka ngesisekelo sofuzo sokutholwa kwezakhi zofuzo ezisebenzayo, ukuhlaziya kokuziphendukela kwemvelo, njll. Esikhundleni sokulandelanisa kabusha kwegenome ejulile, kuncishiswe ukulandelana kofuzo lokumelela (RRGS). ) yethulwa ukuze kuncishiswe izindleko zokulandelanisa isampula ngayinye, kuyilapho kugcinwe ukusebenza kahle okunengqondo ekutholweni komaka wofuzo.Lokhu ngokuvamile kufinyelelwa ngokukhipha isinqamu esikhawulelwe ngaphakathi kobubanzi obunikeziwe bosayizi, obuqanjwe ngokuthi umtapo wolwazi oncishisiwe (RRL).I-Specific-locus amplified fragment sequencing (SLAF-Seq) isu lokuzithuthukisa le-SNP genotyping ene-reference genome noma ngaphandle kwayo.
Ipulatifomu: I-Illumina NovaSeq Platform -
I-Illumina ne-BGI
Ubuchwepheshe bokulandelana kwe-Illumina, obusekelwe ku-Sequencing by Synthesis (SBS), iwukuqanjwa okusha kwe-NGS okwamukelwa emhlabeni wonke, okubophezelekile ukukhiqiza ngaphezu kuka-90% wedatha yokulandelana komhlaba.Umgomo we-SBS ubandakanya ukuthwebula izithombe ezishintshashintshayo ezilebulwe ngokushintshashintsha kwe-fluorescent njengoba i-dNTP ngayinye yengezwa, futhi kamuva igoqe ukuze kuvunyelwe ukufakwa kwesisekelo esilandelayo.Ngawo wonke ama-dNTP amane abophezelayo abophezelayo akhona kumjikelezo ngamunye wokulandelana, ukuncintisana kwemvelo kunciphisa ukuchema kokufakwa.Lobu buchwepheshe obuhlukahlukene busekela imitapo yolwazi efundwa kanye kanye naleyo ebhanqiwe, ihlinzekela uhla lwezinhlelo zokusebenza ze-genomic.Amakhono we-Illumina wokulandelanisa okuphuma phambili nokunemba akubeka njengesisekelo ocwaningweni lwe-genomics, kunikeza ososayensi amandla okuqaqa ubunkimbinkimbi bezakhi zofuzo ngemininingwane engenakuqhathaniswa nokusebenza kahle.
I-DNBSEQ, eyakhiwe yi-BGI, ingobunye ubuchwepheshe be-NGS obusha obukwazile ukwehlisa ngokuqhubekayo izindleko zokulandelana nokwandisa ukusebenza.Ukulungiswa kwemitapo yolwazi ye-DNBSEQ kuhilela ukuhlukaniswa kwe-DNA, ukulungiswa kwe-ssDNA kanye nokukhulisa isiyingi esigoqayo ukuze kutholwe ama-nanoballs e-DNA (DNB).Lezi zibe sezilayishwa endaweni eqinile bese zilandelana ngokulandelana nge-Probe-Anchor Synthesis (cPAS).
Isevisi yethu yokulandelana kwelabhulali eyenziwe ngaphambilini isiza amakhasimende ekulungiseleleni amalabhulali awo alandelanayo avela emithonjeni eyahlukene (mRNA, whole genome, amplicon, phakathi kokunye).Ngokulandelayo, le mitapo yolwazi ingathunyelwa ezikhungweni zethu zokulandelanisa ukuze zilawule ikhwalithi nokulandelana kuzingxenyekazi ze-Illumina noma ze-BGI.
