I-Spatial transcriptomics imi phambili ekusungulweni kwesayensi, inika amandla abacwaningi ukuthi bahlole amaphethini enkulumo eyinkimbinkimbi yezakhi zofuzo phakathi kwezicubu kuyilapho belondoloza isimo sabo sendawo.Phakathi kwamapulatifomu ahlukahlukene, i-BMKGene ithuthukise i-BMKManu S1000 Spatial Transcriptome Chip, iziqhayisaukulungiswa okuthuthukisiweyokungu-5µM, ifinyelela kububanzi obuncane beselula, futhi inika amandlaizilungiselelo zokulungiswa kwamazinga amaningi.I-chip ye-S1000, equkethe cishe izindawo eziyizigidi ezingu-2, isebenzisa ama-microwell agqitshwe ngobuhlalu obulayishwe ama-probe wokuthwebula anebhakhodi yendawo.Umtapo wezincwadi we-cDNA, ohlanganiswe namabhakhodi wendawo, ulungiswa kusukela ku-chip ye-S1000 bese ulandelana ngokulandelana endaweni ye-Illumina NovaSeq.Inhlanganisela yamasampula anebhakhodi yendawo kanye nama-UMI kuqinisekisa ukunemba nokucaciswa kwedatha ekhiqizwayo.Isibaluli esiyingqayizivele se-chip ye-BMKManu S1000 silele ekusebenziseni kwayo izinto ezihlukahlukene, enikeza izilungiselelo zokuxazulula amaleveli amaningi ezingashunwa kahle kumathishu ahlukene namazinga emininingwane.Lokhu kuzivumelanisa nezimo kubeka i-chip njengenketho evelele yezifundo ezihlukahlukene ze-spatial transcriptomics, iqinisekisa ukuhlangana okunembayo kwendawo okunomsindo omncane.
Besebenzisa i-chip ye-BMKManu S1000 nobunye ubuchwepheshe be-transcriptomics yendawo, abacwaningi bangathola ukuqonda okungcono kwenhlangano yendawo yamangqamuzana kanye nokusebenzisana okuyinkimbinkimbi kwamangqamuzana okwenzeka ngaphakathi kwezicubu, kunikeze ukuqonda okubalulekile kwezinqubo eziyisisekelo zezinqubo zebhayoloji emikhakheni eminingi ehlukahlukene, kufaka phakathi. i-oncology, i-neuroscience, i-development biology, i-immunology kanye nezifundo ze-botanical.
I-Platform: BMKManu S1000 chip kanye ne-Illumina NovaSeq