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Ukulandelana kwe-Metagenomic -NGS
I-Metagenome ibhekisela eqoqweni lengqikithi yezinto zofuzo zomphakathi oxubile wezinto eziphilayo, njenge-metagenome yemvelo, i-metagenome yomuntu, njll. Iqukethe ama-genome akho kokubili amagciwane aphilayo nangatshalwa.Ukulandelana kwe-Metagenomic iyithuluzi lamangqamuzana elisetshenziselwa ukuhlaziya izinto ezixubile ze-genomic ezikhishwe kumasampula emvelo, okunikeza ulwazi oluningiliziwe ngokuhlukahluka kwezinhlobo kanye nobuningi, ukwakheka kwabantu, ubudlelwano be-phylogenetic, izakhi zofuzo ezisebenzayo kanye nenethiwekhi yokuxhumana nezici zemvelo.
Inkundla:I-Illumina NovaSeq Platform
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Ukulandelana kwe-Metagenomic-Nanopore
I-Metagenomics iyithuluzi lamangqamuzana elisetshenziselwa ukuhlaziya izinto ezixubile ze-genomic ezikhishwe kumasampula emvelo, ezihlinzeka ngolwazi oluningiliziwe ngokuhlukahluka kwezinhlobo kanye nobuningi, ukwakheka kwabantu, ubudlelwano be-phylogenetic, izakhi zofuzo ezisebenzayo kanye nenethiwekhi yokuxhumana enezici zemvelo, njll. Amapulatifomu okulandelana kwe-Nanopore asanda kwethulwa kwizifundo ze-metagenomic.Ukusebenza kwayo okuhle kakhulu kubude bokufunda kuthuthukise kakhulu ukuhlaziywa kwe-metagenomic yokusakaza, ikakhulukazi ukuhlanganiswa kwe-metagenome.Ngokuthatha izinzuzo zobude bokufunda, ucwaningo lwe-metagenomic olusekelwe ku-Nanopore luyakwazi ukuzuza umhlangano oqhubekayo uma uqhathaniswa ne-shot-gun metagenomics.Kushicilelwe ukuthi i-metagenomics esekwe e-Nanopore ikhiqize ngempumelelo ama-genomes ebhaktheriya aphelele futhi avaliwe avela kuma-microbiomes (Moss, EL, et. al,I-Nature Biotech, 2020)
Inkundla:I-Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
I-subunit ku-16S kanye ne-18S rRNA equkethe zombili izifunda ezilondolozwe kakhulu neziguquguqukayo iwuphawu lweminwe oluphelele lwemolekyuli yokuhlonza i-prokaryotic kanye ne-eukaryotic organisms.Ngokusebenzisa ithuba lokulandelanisa, lawa ma-amplicon angaqondiswa ngokusekelwe ezingxenyeni ezilondoloziwe futhi izifunda eziguquguqukayo zingabonakaliswa ngokugcwele ukuhlonza i-microbial enomthelela ocwaningweni oluhlanganisa ukuhlaziywa kwe-microbial diversity, taxonomy, phylogeny, njll. I-molecule ye-Single-real-time(SMRT) ) ukulandelana kweplathifomu ye-PacBio kwenza kutholwe ukufundwa okude okunembe kakhulu, okungahlanganisa ama-amplicon anobude obugcwele (cishe u-1.5 KB).Ukubuka okubanzi kwendima yofuzo kuthuthukise kakhulu ukulungiswa kwesichasiselo sezinhlobo kubhaktheriya noma emphakathini wesikhunta.
Inkundla:I-PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
Ukulandelana kwe-amplicon ye-16S/18S/ITS kuhloswe ekudaluleni i-phylogeny, taxonomy, kanye nezinhlobo eziningi emphakathini we-microbial ngokuphenya imikhiqizo ye-PCR yezimpawu zofuzo zokugcina izindlu eziqukethe zombili izingxenye ezixoxwa kakhulu nezingaguquguquki.Ukwethulwa kwalezi zigxivizo zeminwe zamangqamuzana aphelele ngu-Woeses et al,(1977) kunika amandla ukuhlukaniswa kwephrofayili ye-microbiome.Ukulandelana kwe-16S (ibhaktheriya), i-18S (isikhunta) kanye ne-Internal transcribed spacer(ITS, isikhunta) kuvumela ukukhonjwa kwazo zombili izinhlobo zezinhlobo eziningi kanye nezinhlobo ezingavamile nezingaziwa.Lobu buchwepheshe sebuphenduke ithuluzi elisetshenziswa kabanzi futhi elikhulu ekuhlonzeni ukwakheka kwe-microbial ehlukile ezindaweni ezahlukahlukene, njengomlomo womuntu, amathumbu, indle, njll.
Inkundla:I-Illumina NovaSeq Platform
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Ukulandelana Kabusha Kwe-Genome Yamagciwane kanye Nesikhunta
Ukulandelana kabusha kwe-genome yegciwane kanye nesikhunta kuyithuluzi elibalulekile lokuqedela ama-genomes amagciwane aziwayo nesikhunta, kanye nokuqhathanisa ama-genome amaningi noma ukwenza imephu yezakhi zezinto eziphilayo ezintsha.Kubaluleke kakhulu ukulandelana kwawo wonke ama-genome e-bacterium nesikhunta ukuze kukhiqizwe ama-genome ayinkomba anembile, ukwenza ukuhlonza ama-microbial kanye nezinye izifundo zokuqhathanisa zofuzo.
I-Platform:I-Illumina NovaSeq Platform
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Ukulandelana kwe-Metatranscriptome
Ukulandelana kwe-Metatranscriptome kukhomba ukubonakaliswa kofuzo kwamagciwane (kokubili i-eukaryote nama-prokaryote) endaweni yemvelo (okungukuthi, inhlabathi, amanzi, ulwandle, indle, namathumbu.). Ngokukhethekile, lezi zinsizakalo zikuvumela ukuthi uthole iphrofayili yesisho sofuzo esiphelele semiphakathi yamagciwane ayinkimbinkimbi, ukuhlaziywa kwe-taxonomic. kwezinhlobo zezilwane, ukuhlaziya okunothisayo kokusebenza kwezakhi zofuzo ezivezwe ngendlela ehlukile, nokunye.
Ipulatifomu: I-Illumina NovaSeq Platform
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I-Fungal Genome
I-Biomarker Technologies ihlinzeka ngenhlolovo ye-genome, i-genome enhle kanye ne-pene-complete genome yefungal kuye ngenhloso ethile yocwaningo.Ukulandelana kwe-genome, ukuhlanganisa kanye nesichasiselo esisebenzayo kungafinyelelwa ngokuhlanganisa ukulandelana kwesizukulwane Esilandelayo + Ukulandelana kwesizukulwane sesithathu ukuze kuzuzwe ukuhlanganiswa kwegenome kwezinga eliphezulu.Ubuchwepheshe be-Hi-C bungaphinda buqashwe ukuze kube lula ukuhlangana kwe-genome ezingeni le-chromosome.
Inkundla:I-PacBio Sequel II
I-Nanopore PromethION P48
I-Illumina NovaSeq Platform
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Amagciwane Aphelele I-Genome
I-Biomarker Technologies ihlinzeka ngensizakalo yokulandelanisa ekwakheni i-genome ephelele yebhaktheriya enegebe eliziro.Ukuhamba komsebenzi okuyinhloko kwebhaktheriya ukwakhiwa okuphelele kofuzo kufaka phakathi ukulandelana kwesizukulwane sesithathu, ukuhlanganisa, isichasiselo esisebenzayo kanye nokuhlaziywa okuthuthukisiwe kwe-bioinformatic okufeza imigomo ethile yocwaningo.Iphrofayili ebanzi yofuzo lwebhaktheriya inika amandla ukudalulwa kwezinqubo ezibalulekile eziyisisekelo zezinqubo zabo zebhayoloji, okungase futhi kunikeze ireferensi ebalulekile yocwaningo lwe-genomic ezinhlotsheni eziphakeme ze-eukaryotic.
Inkundla:I-Nanopore PromethION P48 + Illumina NovaSeq Platform
I-PacBio Sequel II
