UKUVELA KWEGENOME
I-PNAS
Umsuka wokuziphendukela kwemvelo kanye nomlando wokufuywa kwe-goldfish (Carassius auratus)
PacBio |Ilumina |Imephu ye-Bionano Genome |I-Hi-C Genome Assembly |Imephu Yofuzo |GWAS |I-RNA-Seq
Amaphuzu avelele
I-1.Goldfish genome yabuyekezwa ngenguqulo yokuhlanganisa yekhwalithi ephezulu, igxilisa u-95.75% wama-contigs kuma-pseudochromosomes angu-50 (Scaffold N50=31.84 Mb).Ama-subgenomes amabili ahlukaniswe.
2.Izifunda ze-Genomic zokushanelwa okukhethekile ngesikhathi sokufuywa kwasekhaya zihlonzwe kusukela ekulandeleni idatha yabantu abangu-201, kwembulwa kofuzo lwamakhendidethi angaphezu kuka-390 okungenzeka ahlotshaniswa nezici zasekhaya.
I-3.GWAS ku-dorsal fin ku-goldfish yasekhaya iveze izakhi zofuzo ezingama-378 okungenzeka zihlotshaniswe.Intatheli ye-tyrosine-protein kinase ikhonjwe njengofuzo lwecausal ikhandidethi elihlobene nokuba sobala
Ingemuva
I-Goldfish (i-Carassius auratus) ingenye yezinhlanzi ezifuywayo ezibaluleke kakhulu, ezazifuywa kusuka ku-crucian carp e-China yasendulo.Baphawulwe nguCharles Darwin njengokuthi "Sidlula cishe ukuhlukahluka okungapheli kombala, sihlangabezana nezinguquko ezingavamile kakhulu zesakhiwo".Izici ezihluke kakhulu nomlando omude wokufuywa kanye nokuzalanisa kwenza i-goldfish ibe uhlelo oluhle kakhulu lwemodeli yofuzo yefiziyoloji yezinhlanzi nokuziphendukela kwemvelo.
Izimpumelelo
I-Goldfish genome
Jukuhlaziywa kwe-oint kwedatha yokulandelana kwe-PacBio ne-Illumina kuveza umhlangano wokuqala wokusalungiswa ongu-1.657 G (Contig N50=474 KB).Imephu yokubona ye-Bionano yakhiqizwa futhi yalungisa ukuhlanganisa kusayizi ongu-1.73 Gb (Usayizi olinganiselwe we-genome: 1.8 Gb).Umhlangano osuselwe ku-Hi-C uphinde wathuthukisa isikafula i-N50 sisuka ku-606 Kb saya ku-31.84 Mb futhi sazuza isilinganiso esingu-95.75% (1.65 Gb) esiqondiswe noku-oda sokubambelela.I-genome ihlanganisa izakhi zofuzo zekhodi ezingu-56,251 kanye nokulotshiweyo okude okungafaki amakhodi okungu-10,098.Ngaphezu kwalokho, izifunda ezingaba yi-centromeric ezingaba ngu-38 zabikezelwa kuma-chromosome angu-50.
Fig.1 I-Gold fish Genome
Two amasethi acacile we-subgenomes akhonjwe kuma-chromosome e-goldfish angu-50 aba umphumela wesenzakalo sasendulo sokuhlanganisa.Isethi yama-chromosome anengxenye ephezulu yokufunda eqondaniswe phakathi kwe-goldfish ne-Barbinae achazwa njenge-subgenome A (ChrA01~A25), okungukuthi i-subgenome evamile ku-Barbinae, futhi okusele njenge-subgenome B (ChrB01~B25).
Ukushanela kwasekhaya nokukhetha
Aisamba esingu-16 sohlobo lwasendle lwe-crucian carps kanye nezinhlobonhlobo ze-goldfish ezimele eziyi-185 ziye zalandelana ngokujula kokulandelana okumaphakathi okucishe kube ngu-12.5X, okukhiqiza ama-terabase angu-4.3 edatha.Ukwakhiwa kabusha kwe-Phylogenetic kanye nokuhlaziywa kwe-PCA kuqinisekise ukuhlobana okuseduze phakathi kwe-goldfish evamile kanye ne-crucian carp kunezinye izinhlanzi zegolide, ezahlukaniswa zaba imigqa emibili.
LUkuhlaziywa kokubola kwe-D okungaphezulu kwenani elincane labantu abane kwasekela ukuba khona kwezinkinga zofuzo zabantu ngesikhathi sokufuywa kanye nokukhetha okunamandla kokwenziwa kwezinhlanzi zegolide.Ukwenyuka kwezinhlobonhlobo zofuzo (π) ukusuka ku-crucian carp kuya ku-goldfish evamile ukuya ku-Wen goldfish kanye ne-Egg goldfish kubonise ukunqwabelana okubalulekile kokuhlukahluka kofuzo phakathi nokufuywa kwazo.Izifunda ezikhethiwe ezingama-50 ze-genomic ezihlanganisa u-25.2 Mb kanye nezakhi zofuzo ezingama-946 zihlonzwe kudatha yabamele (33 goldfish kanye 16 crucian crap).Ukunweba ukuhlaziya kubantu abangu-201, izakhi zofuzo ezingama-393 zibonise izifunda zokushanela okukhethiwe okuqediwe.Lezi zakhi zofuzo zitholwe zokuhlukahluka okuphansi, okungenzeka kube nomthelela kuma-phenotypes ahlobene nezici ezinkulu zokufuywa kwezinhlanzi zegolide.
I-Fig.3 Ukuhlaziywa okuhlobene ne-Genome-wide domestication
I-GWAS kuzinhlanzi zegolide ezifuywayo
Di-orsal fin isici esibalulekile esihlukanisa i-Wen goldfish ne-Egg goldfish.I-GWAS ye-dorsal fin ku-96 Wen goldfish kanye ne-87 Egg goldfish iveze izakhi zofuzo ezingu-378 ezisabalele kuma-chromosome angu-13 futhi kwaqashelwa ukusatshalaliswa okungalingani kwalezi zakhi zofuzo phakathi kwama-subgenomes.Ukuhlaziywa kokusebenza kwalezi zakhi zofuzo zamakhandidethi kugqamise izinqubo zebhayoloji ezihlanganisa “ukusayinda kwe-Cell surface receptor”, “Transmembrane transportation”, “Skeletal system development”, njll.
Umdwebo we-4 GWAS we-dorsal fin kwi-goldfish efuywayo
In I-GWAS yezici ezihlobene nesikali esisobala, kutholwe inani eliphakeme lokuhlangana eliqinile.Ufuzo olufaka ikhodi ye-tyrosine-protein kinase receptor lukhonjwe endaweni eyodwa yekhandidethi.
I-Fig.5 GWAS yezici ezihlobene nesikali esisobala
Ireferensi
Chen D et al.Umsuka wokuziphendukela kwemvelo kanye nomlando wokufuywa kwe-goldfish (Carassius auratus).I-PNAS (2020)
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Isikhathi sokuthumela: Jan-04-2022