Ngo-2021, i-BMKGENE yabona ucwaningo lwama-31 lwe-De novo genome olushicilelwe ngempumelelo kumajenali anethonya elikhulu anezici zomthelela eziphelele ngaphezu kwama-320. Ezingu-15 zezindatshana zabhalwa ngokuhlanganyela 4 yazo yabhalwa ngokuhlanganyela njengababhali bokuqala yi-BMKGENE.
Ngemva nje kokuqala konyaka wezi-2022, sekuvele kunezindatshana ezimbili zocwaningo ezishicilelwe kujenali ethi “Natural Genetics” kanye “Nesitshalo Semolekyuli” ngokulandelana.Zithi, “Ama-haplotype amabili ahlukene avela ku-heterozygous lychee genome kakhulu aphakamisa izehlakalo ezizimele zokufuywa kwezinhlobo zezitshalo ezisheshe zivuthwe” (ucwaningo lwe-Lychee genome, olwenziwa yi-College of Horticulture, inyuvesi yezoLimo eNingizimu neChina, kanye nabahlanganyeli besayensi, olushicilelwe ku-Natural Genetics. ), kanye “Ukulandelana kweGenome kwezinhlobo ezinhlanu ze-Sitopsis ze-Aegilops kanye nomsuka we-polyploid kakolweni i-B-subgenome” (Izinhlobo ze-Five Sitopsis genome, olwenziwa ithimba locwaningo likaProfessor Bao Liu laseNortheast Normal University.).Sizophinde sibukeze lezi zihloko ezimbili futhi sabelane nabafundi bethu.
Manje, ake sibheke izindatshana ezinhle kakhulu zocwaningo ezishicilelwe ngo-2021 ezibhalwe ngokuhlanganyela yi-BMK kanye nezindawo zethu ezihlanganyelwe.
I-Genome Yezitshalo - Ukuthuthukiswa kwezinhlobo eziningi zezinhlobo.
1.Ukuhlanganiswa kofuzo lwekhwalithi ephezulu kugqamisa izici ze-rye genomic kanye nezakhi zofuzo ezibalulekile zezolimo
Isikhungo Esihlanganyelwe: I-Henan Agricultural University
Ijenali: I-Natural Genetics
Isici Somthelela: 38.31
Kule phrojekthi, i-genome ye-Weining rye, i-elite Chinese rye, ihlelwe ngokulandelana.Ama-contigs ahlanganisiwe (7.74 Gb) abala u-98.47% wosayizi olinganiselwe we-genome (7.86 Gb), no-93.67% wama-contigs (7.25 Gb) anikezwe ama-chromosome ayisikhombisa.Izakhi eziphindaphindayo zenze u-90.31% we-genome ehlanganisiwe.Ukuhlaziywa okwengeziwe komhlangano we-Weining kuveza ukukhanya okusha ekuphindaphindweni kofuzo okubanzi kofuzo kanye nomthelela wako kuzakhi zofuzo zesitashi se-biosynthesis, ukuhlelwa ngokomzimba kwe-prolamin loci eyinkimbinkimbi, izici zesimo sofuzo eziyisisekelo sesihloko sokuqala kanye nezifunda ze-chromosomal ezihlotshaniswa nokufuywa ekhaya kanye ne-loci ku-rye.Lokhu kulandelana kwe-genome kuthembisa ukusheshisa izifundo ze-genomic kanye nokuzalanisa ku-rye kanye nezitshalo zokusanhlamvu ezihlobene.
I-2.I-Rose ngaphandle kwe-prickle: ukuqonda kwe-genomic okuhlotshaniswa nokujwayela umswakama
Isikhungo Esihlanganyelwe: I-Kunming Institute of botany, i-Chinese Academy of Sciences
Ijenali: Ukubuyekezwa Kwesayensi Kazwelonke
Isici Somthelela: 17.273
Kule phrojekthi, amasampula e-'Basye's Thornless' (BT, i-cultivar engenawo ama-prickle ye-Rosa wichuraiana), 'Old Blush' (OB, i-genotype yomsunguli ekufuyweni kwama-rose), ama-hybrids awo e-F1 kanye ne-BCF1 aqoqiwe.Futhi umhlangano we-genome oyinkomba wekhwalithi ephezulu wakhiqizwa ukukhomba izici zofuzo ezihlobene nokuthuthukiswa kwe-stem prickle.Usayizi we-genome cishe u-530.6 Mb.Ukuqinisekisa ikhwalithi ye-genome ehlanganisiwe, ukuhlaziya okufana nokuqhathanisa imephu yofuzo, i-BUSCO, i-NGS ifunda ukuhlanganisa kabusha, ukuqhathanisa ne-OB haplotype, ukulawulwa kwesilinganiso sephutha elilandelanayo kanye nokuhlolwa kwenani le-genome-wide LTR Assembly Index (LAI=20.03) kwenziwa.Lolu cwaningo lwembula iphethini yefa eyinkimbinkimbi kanye nendlela yokulawula yama-stem prickles futhi lwasinikeza isisekelo nezinsiza ezintsha zokutadisha i-rose biology kanye nemayini omaka wamamolekyuli ahlobene nezici ezibalulekile.
3.Ukulandelana Kwe-Genome Ephelele Ye-Allopolyploid Ehlanganisiwe ku-Cucumis Yembula Imibono Nge-Genome Evolution ye-Allopolyploidization
Isikhungo Esihlanganyelwe: Nanjing Agricultural University
Ijenali: Isayensi Ethuthukisiwe
Isici Somthelela: 16.801
Lolu cwaningo lubike i-genome yekhwalithi ephezulu ye-allotetraploid yokwenziwa etholwe kusetshenziswa ukuxutshwa kwe-interspecific phakathi kwekhukhamba (C. sativus, 2n = 14) kanye nezinhlobo zayo ezihlobene zasendle (C. hystrix, 2n = 24) kanye nokuphindaphinda okulandelayo kwechromosome, okungeyokuqala ilandelana ngokugcwele i-synthetic allopolyploid.Ukuhlanganiswa kwegenome kusebenzise ukugeleza komsebenzi kokulandelana kwe-“PacBio+BioNano+Hi-C+Illumina”, kubangele usayizi wofuzo ongu-530.8Mb kanye ne-contig N50 = 6.5Mb.Ukufundwa kwabelwa ama-pseudochromosomes nama-subgenomes angu-19.Imiphumela ibonise ukuthi ukuxutshwa, esikhundleni sokuphindaphinda i-genome, kubangela iningi lezinguquko ze-genomic kukho kokubili i-nuclear ne-cp genomes.Kuphakanyiswe ukuthi i-heterozygosity engaguquki inikeza i-C.×hytivus ukwanda kokuzivumelanisa nokucindezeleka.Imiphumela inikeza imininingwane emisha mayelana nokuvela kwezitshalo ze-polyploidy futhi inikeza isu lokuzalanisa lezitshalo ezizayo.
4.I-Comparative Genome Ihlaziya Igqamisa Ukunwetshwa kweGenome ye-Transposon Mediated kanye ne-Evolutionary Architecture ye-3D Genomic Folding kukotini
Isikhungo Esihlanganyelwe: Huazhong Agricultural University
Ijenali: Ibhayoloji Yamangqamuzana Nokuziphendukela Kwemvelo
Isici Somthelela: 16.242
Le phrojekthi isebenzise i-Nanopore Sequencing ukuze ihlanganise i-genome yezinhlobo ezintathu zikakotini okuyizi: Gossypium rotundifolium (K2, usayizi wegenome = 2.44Gb, contigN50 = 5.33Mb), G. arboreum (A2, usayizi wegenome = 1.62Gb, 1b = M = 1b 50), kanye G. raimondii (D5, usayizi wegenome = 0.75Gb, contigN50 = 17.04 Gb).Ngaphezu kwama-99% awo womathathu ama-genome ahlanganiswe nge-Hi-C.Imiphumela yokuhlaziywa kweBUSCO ingu-92.5%, 93.9% no-95.4% ngokulandelana.Zonke lezi zinombolo zibonise ukuthi ama-genome amathathu okuhlangana ayireferensi -grade.Ukuhlaziywa kwe-genome okuqhathanisayo kubhale imininingwane yokukhulisa i-TE eqondene nomugqa okufaka isandla kumehluko omkhulu wosayizi we-genome.Lolu cwaningo lunikeza ukukhanya endimeni yokunwetshwa kwe-genome ye-transposon-mediated ekuguqukeni kwesakhiwo se-chromatin esiphezulu ezitshalweni.
5. Ukuhlanganiswa kwesikali seChromosome nokuhlaziywa kwesitshalo se-biomass Miscanthus lutarioriparius genome
Isikhungo Esihlanganyelwe: I-CAS Centre for Excellence in molecular Plant Sciences
Ijenali: Ukuxhumana Kwemvelo
Isici Somthelela: 14.912
Le phrojekthi ibike ukuhlanganiswa kwesikali se-chromosome ye-Miscanthus lutarioriparius genome ngokuhlanganisa ukulandelana kwe-Oxford Nanopore kanye nobuchwepheshe be-Hi-C.Umhlangano we-2.07Gb uhlanganisa i-96.64% ye-genome, ne-contig N50 ye-1.71 Mb.Cishe u-94.30% wokulandelana okuphelele kugxilwe kuma-pseudochromosomes angu-19.Ngokuqhathanisa nokulandelana kwe-BAC, ukuhlolwa kwe-LAI, ukuhlolwa kwe-BUSCO, ukuphinda kuhlanganiswe nedatha ye-NGS, ukuphinda kuhlanganiswe idatha ye-transcriptome, i-genome yahlolwa njengekhwalithi ephezulu nokuqhubeka.Umsuka we-Allotetraploid we-M. lutarioriparius uqinisekiswa kusetshenziswa ukuphindaphinda kwesathelayithi ye-centromeric.Izakhi zofuzo eziyimpinda ye-Tandem ye-M. lutarioriparius ziyasebenza hhayi kuphela ngokwemibandela ehlobene nokusabela kwengcindezi, kodwa i-cell wall biosynthesis.Izimpinda zofuzo cishe zidlala indima ku-C4 photosynthesis futhi zineqhaza ku-Miscanthus C4 photosynthesis ezingeni lokushisa eliphansi.Ucwaningo lunikeze ireferensi ebalulekile yokutadisha izitshalo ezingapheli.
6.Ukuhlanganiswa kwegenome kweleveli ye-chromosome i-Camptotheca acuminata inikeza imininingwane ngemvelaphi yokuvela kwe-camptothecin biosynthesis
Isikhungo Esihlanganyelwe: Inyuvesi yaseSichuan
Ijenali: Ukuxhumana Kwemvelo
Isici Somthelela: 14.912
Le phrojekthi ibike ukuhlanganiswa kwegenome yekhwalithi ephezulu, ye-chromosome ye-C. acuminata, enosayizi we-genome ongu-414.95Mb kanye ne-contingN50 1.47Mb.Sithole ukuthi i-C. acuminata ihlangabezana nokuphindaphindeka kofuzo oluzimele oluphelele futhi izakhi zofuzo eziningi eziphuma kukho zihlobene ne-camptothecin biosynthesis.Ukwehlukana kokusebenza kofuzo lwe-LAMT kanye nokuvela okuhle kwezakhi zofuzo ezimbili ze-SLAS, ngakho-ke, kokubili kube negalelo elikhulu ku-camptothecin biosynthesis ku-C. acuminata.Imiphumela yagcizelela ukubaluleka kokuhlanganiswa kwe-genome yekhwalithi ephezulu ekuboneni izinguquko zofuzo emsuka wokuziphendukela kwemvelo we-metabolite yesibili.
I-7.I-allele-defined genome yembula ukuhlukaniswa kwe-biallelic ngesikhathi sokuvela kwe-cassava
Isikhungo Esihlanganyelwe: I-Chinese Academy of Tropical Agricultural Sciences
Ijenali: Isitshalo samangqamuzana
Isici Somthelela: 13.162
Le phrojekthi ihlanganise i-genome eyinkomba ye-cassava ene-contigN50 1.1Mb isebenzisa inkundla yokulandelana ye-Pacific Biosciences (PacBio).Ngemva kokuhlolwa yi-BUSCO, inkomba ye-LAI, kanye nemephu yofuzo enabantu abaningi, i-genome ehlanganisiwe iqinisekiswa njengebanga lokuvikeleka.Izifunda ezingasasebenzi zikhonjiwe futhi ama-contig agxilwa kuma-pseudochromosomes angu-18 kusetshenziswa izixhumanisi ze-Hi-C.Le genome yereferensi yekhwalithi ephezulu kanye ne-allele echaziwe yomdumbula ibalulekile ekuhlonzeni ama-bi-allele ahlukene kuma-chromosome angama-homologous, okuvumela ukuhlola ukuhlukanisa nokuveza ukubusa kwama-bi-allele namandla awo okushayela okuziphendukela kwemvelo.Kwenze kube lula ukusungula amasu okuzalanisa umdumbula nezinye izitshalo ezine-heterozygous kakhulu.
8.Imibono ye-Genomic ekukhuleni okusheshayo kwe-paulownias kanye nokwakhiwa komshanelo wabathakathi be-Paulownia
Isikhungo Esihlanganyelwe: Inyuvesi yaseHenan Agricultural
Ijenali: Isitshalo samangqamuzana
Isici Somthelela: 13.162
Le phrojekthi ihlanganise i-genome yenuzi yekhwalithi ephezulu ye-Paulownia fortunei, usayizi ongu-511.6 Mb, nama-93.2% wokulandelana agxiliswe kuma-pseudochromosomes angu-20.Ukusebenza kahle kwe-photosynthetic okuphezulu kufinyelelwa ngokuhlanganisa i-C3 photosynthesis kanye nendlela ye-crassulacean acid metabolism, okungenzeka ibe nomthelela emkhubeni wokukhula ngokushesha okukhulu kwezihlahla ze-paulownia.Ukulandelana kofuzo okwengeziwe kwe-PaWB phytoplasma, kuhlanganiswe nokuhlaziywa kokusebenza, kubonise ukuthi umphumela we-PaWB-SAP54 usebenzelana ngokuqondile ne-Paulownia PfSPLa, okubuye kubangele ukucekelwa phansi kwe-PfSPLa ngendlela esetshenziswa yi-ubiquitin futhi kuholela ekwakhekeni komshanelo wabathakathi.Idatha inikeze imininingwane ebalulekile kubhayoloji ye-paulownias kanye nendlela yokulawula yokwakhiwa kwe-PaWB.
I-genome yezilwane - Imibono ejulile yokuvela kwezinhlobo zezilwane
1.I-genome ye-Nautilus pompilius ikhanyisa ukuvela kwamehlo kanye ne-biomineralization
Isikhungo Esihlanganyelwe: I-South China Sea Institute of Oceanology, CAS
Ijenali: Imvelo Engokwemvelo & Evolution
Isici Somthelela: 15.462
Le phrojekthi yethule i-genome ephelele ye-Nautilus pompilius.Ine-genome encane phakathi kwama-cephalopod alandelanayo, angama-730.58Mb ane-contigN50 = 1.1Mb.Umphumela wokuhlola we-BUSCO ungama-91.31%.Ihlanganiswe ne-transcriptome, i-proteome, i-gene family kanye nokuhlaziywa kwe-phylogenetic, le genome inikeze ireferensi eyisisekelo emisha ye-cephalopod, njenge-pinhole eye kanye ne-biomineralization.Ucwaningo luveze ukuthi umonakalo ekupheleleni kweqoqo lofuzo lwe-Hox kungenzeka uhlobane nokunyamalala kwegobolondo lama-Mollusk.Okubalulekile, ukusungulwa okuningi kwe-genomic okuhlanganisa ukulahlekelwa kwezakhi zofuzo, ukufinyela okuzimele kanye nokwanda kwemindeni ethile yofuzo kanye namanethiwekhi okulawula ahlobene nawo okungenzeka abumba ukuvela kweso le-nautilus pinhole.I-nautilus genome yakha insiza ebalulekile yokwakha kabusha izimo zokuziphendukela kwemvelo kanye nokuqanjwa kwe-genomic okwakha ama-cephalopod asekhona.
2.Ukuhlaziywa kofuzo lwe-Seadragon kunikeza imininingwane nge-phenotype yayo kanye nendawo yokunquma ubulili
Isikhungo Esihlanganyelwe: I-South China Sea Institute of Oceanology, CAS
Ijenali: Intuthuko Yesayensi
Isici Somthelela: 14.132
Le phrojekthi ye-novo–ilandelanisa ama-genome owesilisa nowesifazane we-seadragon evamile (Phyllopteryx taeniolatus) kanye nezinhlobo zayo ezihlobene eduze, i-alligator pipefish (Syngnathoides biaculeatus).Usayizi we-genome we-Phyllopteryx taeniolatus ngu-~659 Mb(♂) kanye no-~663 Mb (♀), ne-contigN50 ka-10.0Mb no-12.1mb.usayizi we-genome we-Phyllopteryx taeniolatus ngu-637 Mb(♂) kanye no-~648 Mb (♀), ne-contigN50 yokungu-18.0Mb no-21.0Mb.Ngokuhlaziya i-phylogenetic, i-seadragon evamile kanye ne-alligator pipefish i-taxton engudadewabo yase-Syngnathinae, futhi yahlukana cishe nge-27.3 Ma edlule.Amaphrofayli alotshiweyo avela entweni entsha, ama-appendages afana neqabunga, abonisa ukuthi isethi yezakhi zofuzo ngokuvamile ezibandakanyekayo ekuthuthukisweni kokugcina zikhethiwe kanye nokunothiswa kwemibhalo ebhaliwe ukuze ikwazi ukukhanda izicubu kanye nezakhi zofuzo zokuzivikela komzimba.I-putive sex-determining locus efaka isakhi sofuzo se-amhr2y esiqondene nomuntu wesilisa esabelwa i-seadragon evamile kanye ne-alligator pipefish ikhonjiwe.Le phrojekthi inikeze ubufakazi obubalulekile bocwaningo lokuziphendukela kwemvelo okuguquguqukayo.
Isikhathi sokuthumela: Sep-19-2022