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条形 isibhengezo-03

Izindaba

I-Nanopore-based Full-length Transcriptome Sequencing

Ukulandelana kwe-Nanopore kuzihlukanisa kwezinye izinkundla zokulandelana, ngokuthi ama-nucleotide afundwa ngokuqondile ngaphandle kokuhlanganiswa kwe-DNA futhi akhiqize ukufunda okude emashumini ama-kilobase.Lokhu kunika amandla ukufundwa okuqondile kokulotshiweyo kobude obugcwele kanye nokubhekana nezinselele ezifundweni ze-transcriptomic.

√ Ukuchema okuqondene nokulandelana okuphansi

√ Ukufundwa kobude obugcwele kwe-cDNA kuya ezifundweni zesakhiwo sofuzo

√ Idatha encane edingekayo ukumboza inombolo efanayo yemibhalo ebhaliwe

√ Ukukhonjwa kwama-isoform amaningi isakhi ngasinye

√ Ukulinganisa kwenkulumo kuzinga le-isoform

i-nanopore-1

Ipayipi le-Bioinformatics elijwayelekile

Ubude obugcwele-trans-bix-1024x254

Amacala Aphumelele Akamuva Nge-Biomarker Technologies

1. Ukuhlaziya okuqhathanisayo kwama-transcriptomes anobude obugcwele kuveza ukuguquguquka kwe-Gnetum luofuense stem

Ijenali: Frontiers in Genetics

Kushicilelwe: Mashi 2021

Amagama angukhiye: Minion |Okunye ukuhlanganisa |APA |lncRNA |Okulotshiweyo okuvezwe ngokwehlukile

Funda Umbhalo Ogcwele

2. Ukunyakaza okukodwa kwama-RNA amancane ukusuka emahlumela kuya ezimpandeni kuma-interspecific heterografts

Ijenali: Izitshalo Zemvelo

Kushicilelwe: Jan. 2021

Amagama angukhiye: Nanopore |Ilumina |ukutholwa kwe-mRNA yeselula yobude obugcwele

3. Ukuhlaziywa kwe-transcriptome yobude obugcwele bezimpande ze-asparagus kuveza indlela yamangqamuzana yokubekezelela usawoti okubangelwa isikhunta se-arbuscular mycorrhizal.

Ijenali: Isayensi Yezemvelo Nokuhlola

Kushicilelwe: Jan. 2021

Amagama angukhiye: Minion |Imibhalo ebhalwe ngokwehlukile |Izici zokuloba |Okunye ukuhlanganisa

4. Izinguquko Zesimo Semvelo kanye Nombhalo Obhalwe Ngobude Obugcwele Bezimpande (Rosa chinensis) Izimpande Namaqabunga Ekuphenduleni Kukucindezeleka Kwesomiso

Ijenali: I-Plant and Cell Physiology

Kushicilelwe: Okthoba 2020

Amagama angukhiye: PromethION |Imibhalo ebhalwe ngokwehlukile |Isibikezelo esisebenzayo |Ama-isoform ahlukanisiwe |I-TF kanye ne-lncRNA

5. Ukuhlaziywa nokuqhathanisa okuphelele kwe-PacBio kanye nokulandelana kwe-RNA okusekelwe ku-nanopore kwe-Arabidopsis transcriptome

Ijenali: Indlela Yezitshalo

Kushicilelwe: Ephreli 2020

Amagama angukhiye: Igridi |PromethION |I-PacBio Sequel |Illumina NovaSeq |Ukuqondanisa kwe-genome |Umazisi wombhalo |Okunye ukuhlanganisa |SSR |LncRNA |I-Isoform quantification

Izindaba Namaphuzu Avelele ihlose ukwabelana ngamacala akamuva ayimpumelelo ne-Biomarker Technologies, ithwebula izimpumelelo zesayensi ezinoveli kanye nezindlela ezivelele ezisetshenziswe phakathi nocwaningo.


Isikhathi sokuthumela: Jan-08-2022

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