I-MICROBIAL
Ukuhambisana kwamagciwane kanye nokunganyakazi nge-biochar kuthuthukisa ukuwohloka kwe-tebuconazole, ukwakheka kwe-microbiome yomhlabathi nokusebenza
Ubude obugcwele bokulandelana kwe-amplicon ye-16S |I-PacBio HiFi |Ukuhlukahluka kwe-Alpha |Ukuhlukahluka kwe-Beta
Kulolu cwaningo, ukulandelana kwe-amplicon ye-16S yobude obugcwele yi-PacBio kanye nokuhlaziywa kwe-bioinformatic kunikezwe yi-Biomarker Technologies.
Amaphuzu avelele
I-Biochar immobilised tebuconazole-degrading bacteria I-Alcaligenes faecalis WZ-2 yacwaningwa ngokusebenza kahle kokubola kwe-biodegradation futhi ithinta inhlabathi engcolile ye-tebuconazole uma kuqhathaniswa nohlobo lwamahhala oluwohlokayo lwe-WZ-2.
1. I-Biochar-immobilized WZ-2 ibonise ukuwohloka okusebenza kahle kakhulu ku-tebuconazole uma kuqhathaniswa ne-WZ-2 yamahhala ngokunciphisa uhhafu wempilo ye-tebuconazole enhlabathini kusukela ezinsukwini ezingu-18.7 kuya ezinsukwini ezingu-13.3.
2. I-Biochar-immobilized WZ-2 ikwazile ukubuyisela imisebenzi ye-enzyme ye-microbial enhlabathini, okuhlanganisa i-urease, i-dehydrogenase ne-invertase, njll.
3. Iphrofayili ye-Microbial enhlabathini ephathwe nge-biochar-immobilized WZ-2 enqunywa ukulandelana kobude obugcwele be-16S isekelwa ngokuqinile ukuthi lolu hlelo lungabuyisela impilo yomhlabathi ngokwenza ngcono ukwakheka komphakathi wamagciwane ngaphansi kokungcoliswa kwe-tebuconazole.
Ukuhlola (okuhlobene nokulandelanisa)
Amaqembu: CK: Inhlabathi yemvelo;T: Inhlabathi efakwe i-tebuconazole;S: I-Tebuconazole inhlabathi equkethe i-WZ-2 yamahhala;BC: I-Tebuconazole equkethe inhlabathi ene-biochar;I-BCS: I-Tebuconazole yayiqukethe inhlabathi ene-biochar immobilized WZ-2.
Isampula: Isamba sokukhishwa kwe-DNA yenhlabathi ekhuliswe ngama-primer angu-16S rDNA
27F (5′-AGAGTTTGATCCTGGCTCAG-3′) kanye ne-1492R (5′-GGTTACCTTGTTACGA),repesenting 16S rDNA yobude obugcwele
Inkundla yokulandelanisa: PacBio RS II
Isu lokulandelanisa: I-CCS HIFI ifundeka
Ukuhlaziywa kwedatha:BMKCloudI-Bioinformatic Platforming yenza i-goldfish ibe uhlelo oluhle kakhulu lwemodeli yofuzo yefiziyoloji yezinhlanzi nokuziphendukela kwemvelo.
Umphumela
Isakhiwo somphakathi we-microbial yomhlabathi sanqunywa ukulandelana kwe-16S rDNA.Inkomba ye-OTU yokunotha nokuhlukahluka kwe-alpha, okuhlanganisa nezinkomba ze-Chao1, Ace, Shannon kanye ne-Simpson ziye zahlolwa ukuze kwembulwe ukuhlukahluka kwezinhlobo zezinhlobo ohlelweni ngalunye.Ngemva kwezinsuku ezingu-60 ekufukameleni, zonke izinkomba ezikhonjiswe inkambiso efanayo, okungukuthi i-tebuconazole ingadala ukuncipha kokunotha kwezinhlobo kanye nokuhlukahluka enhlabathini.Kodwa-ke, ngokwengeza uhlobo lwe-WZ-2, umphakathi wamagciwane enhlabathi watholakala ngokwengxenye yakho kokubili ukunotha nokwehlukahlukana.Umehluko olinganiselwe wabonwa phakathi kwe-BC, BCS kanye ne-CK, okubonisa ukuthi i-biochar ne-biochar-immobilized WZ-2 ingabuyisela ngempumelelo impilo yebhayoloji yenhlabathi.
Indlela Yeqembu Elibili Elingenasisindo Ngezindlela Ze-Arithmetic (UPGMA) isetshenziswe kulolu cwaningo ukuze kuboniswe ukuhlukahluka kwe-beta phakathi kwamaqembu.Njengoba kuboniswe emfanekisweni olandelayo, i-BC, i-BSC kanye ne-CK babelane ngephethini efanayo yokubunjwa kwamagciwane uma kuqhathaniswa neqembu T kanye no-S, okuphinde kwabonisa ukuthi ukwethulwa kwe-biochar ku-bioremediation yenhlabathi engcoliswe yi-tebuconazole kungasiza kakhulu ukutholwa komphakathi wamagciwane emhlabathini.
Umfanekiso.Ukuqoqwa kwe-UPGMA komphakathi wamagciwane ezingeni le-phylum ngaphansi kokwelashwa okuhlukile
Ireferensi
Sun, Tong, et al."Ukuhambisana kwamagciwane kanye nokunganyakazi nge-biochar kuthuthukisa ukuwohloka kwe-tebuconazole, ukwakheka kwe-microbiome yomhlabathi nokusebenza."Ijenali Yezinto Eziyingozi398 (2020): 122941.
Izindaba Namaphuzu Avelele ihlose ukwabelana ngamacala akamuva ayimpumelelo ne-Biomarker Technologies, ithwebula impumelelo yesayensi yenoveli kanye namasu avelele asetshenziswa phakathi nocwaningo.
Isikhathi sokuthumela: Jan-08-2022