I-GWAS
Isihloko: Ukulandelanisa kabusha kwegenome yonke kuveza imvelaphi ye-Brassica napus kanye ne-genetic loci ehilelekile ekuthuthukisweni kwayo
Ijenali: Ukuxhumana Kwemvelo
NGS |WGS |Ilandelana |GWAS |I-Transcriptome |RNASEK |Brassica napus |Evolution |Ezasekhaya
Kulolu cwaningo, i-Biomarker Technologies ihlinzeke Ngezinsizakalo zokulandelana kwe-NGS, kanye nokusekelwa kwezobuchwepheshe ekuhlaziyweni kwe-bioinformatics ekulandeleni idatha.
Ingemuva
I-Brassica napus(i-rapeseed) iyisitshalo esibalulekile sembewu kawoyela futhi iyimodeli enhle kakhulu yokuphenya izinqubo ze-polyploid speciation, ukuziphendukela kwemvelo nokukhetha.Kodwa-ke, ukuthi izilwane zasendle noma abanikeli abafuywayo babengabazala babazali kanye nezakhi zofuzo ezibambe iqhaza ekufuyweni nasekuthuthukisweni kwembewu yokudlwengulwa akwaziwa.
Izimpahla nezindlela
Izinto zokwakha:588B. napusUkufinyelela kubandakanyeke kulolu cwaningo, okubandakanya abangama-466 base-Asia, abangu-102 base-Europe, abangu-13 baseNyakatho Melika, nabangu-7 base-Australia.Ngokusekelwe kumarekhodi emikhuba yokukhula, lezi zinto zahlukaniswa zaba ama-ecotypes amathathu;intwasahlobo (ama-86 accessions), ubusika (74 accessions), kanye ne-semi-winter (428 accessions).
Ukulandelanisa:Isilinganiso esilinganiselwe.5× (kusukela ku-3.37× kuya ku-7.71×)
Inkundla yokulandelanisa:Illumina Hiseq 4000
Ukukhiqizwa kwedatha:4.03 Tb idatha ehlanzekile
Ukushaya kwe-SNP:I-BWA + GATK.Kutholwe ama-SNP angu-5,294,158 kanye nama-InDels angu-1,307,151.
Imiphumela
Umsuka we-B. napus
B. napusI-subgenome yavela kukhokho wetheniphu yaseYurophu.Umcimbi wokugeleza kofuzo kusuka kutheniphu yaseYurophu kuya e-B. napuI-subgenome yenzeka eminyakeni engu-~106–1170 edlule.B. napusI-subgenome engu-C kungenzeka ukuthi yavela kukhokho oyedwa walezi zigaba zozalo.Idlozi likaB. napusukuhlukana nokhokho ovamile wezinhlobo ezine ze- B. oleracea, ngokugeleza kofuzo lwakamuvaB. napus~ Iminyaka engu-108–898 edlule.B. napusI-subgenome ye-C inomsuka oyinkimbinkimbi kune-A Subgenome.Ibhodlela eliqinile lakhiwe kuwo womabili ama-subgenomes phakathiB. napusukuziphendukela kwemvelo.Ubusika kanye semi-busikaB. napusama-ecotypes ahlukene ~ eminyakeni engama-60 edlule, kanti ubusika nentwasahloboB. napusyahluka ~ eminyakeni engu-416 edlule, kanye nembewu yamafutha kanye ne-non-oilseedB. napusyahluka ~ eminyakeni engama-277 edlule.
Umfanekiso 2 Ukwakheka kwesibalo sabantu abangama-588 B. ukungena kwe-napus kanye ne-199 ye-B. rapa accessions.
Umdwebo 3 Ukwakheka kwenani labantu abangu-588 B. ukungena kwe-napus kanye ne-119 ye-B. oleracea accessions
Izimpawu zokukhetha kanye nezifundo zenhlangano ye-Genome-wide.
Phakathi nesigaba sokuqala sokuthuthukiswa (i-FSI), ukuhlukahluka kwezakhi zofuzo okwengeziwe kulahleke ku-subgenome ye-B. napus C kune-subgenome engu-A.Ukuhlukaniswa okuncane kofuzo kwenzeka ngesikhathi se-FSI kunangesikhathi sesigaba sesibili sokuthuthukiswa (SSI).Izakhi zofuzo ezifundeni zesignali zokukhetha i-SSI zathuthukiswa ekubekezeleleni ukucindezeleka, ukuthuthukiswa kanye nezindlela ze-metabolic.Ama-loci angama-60 ahlotshaniswa kakhulu nezici ezihlosiwe eziyi-10, okubandakanya ezi-5 ezihlobene nesivuno sembewu, ubude obu-3 kuya ku-silique, 4 kokuqukethwe kawoyela, kanye nekhwalithi yembewu engu-48 zihlonziwe.
Umdwebo 4 ukuskena okubanzi kwe-Genome nezichasiselo zezifunda ezikhethiwe ngesikhathi se-SSI ye-B. napus
Ukuhlaziywa kwe-Transcriptome
Idatha ye-RNAseq yamathishu angu-11 avela ku-cultivar equkethe amafutha amaningi kanye ne-double-low kanye nezinhlobo zofuzo ezihlonziwe ezinokuqukethwe okuphansi kwamafutha kanye ne-double high ezihlobene nenqubo ye-glucosinolate biosynthetic zazimelwe kakhulu.
Umdwebo 5 Uhlolojikelele lomthetho wesikhathi sokuqhakaza ngaphansi kokukhethwa kokuthuthukiswa kwe-ecotype ye-B. napus
Ingxoxo
Lolu cwaningo lunikeze insiza ebalulekile yokuqonda umsuka nomlando wokuthuthukisaB. napusfuthi kuzokwenza kube lula ukuhlukaniswa kwezisekelo zofuzo zezici ezibalulekile ze-agronomic complex.Ama-SNP abalulekile ahlotshaniswa nokuhlukahluka okuhle, amasignali okukhetha kanye nezakhi zofuzo zamakhandidethi azofaka isandla kakhulu esikhathini esizayo, ikakhulukazi ekuthuthukiseni isivuno, ikhwalithi yembewu, okuqukethwe kukawoyela, kanye nokuvumelana nezimo kwalesi sitshalo sakamuva se-allopolyploid nezihlobo zaso.
Ireferensi
Ukulandelanisa kabusha kwe-whole-genome kuveza imvelaphi ye-Brassica napus kanye ne-genetic loci ehilelekile ekuthuthukisweni kwayo[J].Ukuxhumana Kwemvelo, 2019, 10(1).
Izindaba Namaphuzu Avelele ihlose ukwabelana ngamacala akamuva ayimpumelelo ne-Biomarker Technologies, ithwebula izimpumelelo zesayensi ezinoveli kanye nezindlela ezivelele ezisetshenziswe phakathi nocwaningo.
Isikhathi sokuthumela: Jan-05-2022