UMHLANGANO WEGENOME we-T2T, I-GAP YAMAHHALA I-GENOME
1stAmaGenome Elayisi Amabili1
Isihloko: Umhlangano Nokuqinisekiswa Kwezinhlobo Ezimbili Zereferensi Ezingenayo I-Gap ze-Xian/indica Rice Yembula Imininingwane Nge-Plant Centromere Architecture
I-Doi:https://doi.org/10.1101/2020.12.24.424073
Isikhathi Sokuthunyelwa: Janawari 01, 2021.
Isikhungo: Huazhong Agricultural University, China
Izinto zokwakha
O. sativa xian/indicaizinhlobo zelayisi 'Zhenshan 97 (ZS97)' kanye 'Minghui 63 (MH63)
Isu lokulandelanisa
I-NGS ifunda + i-HiFi ifundeka + i-CLR ifundeka + BioNano + Hi-C
Idatha:
ZS97: 8.34 Gb (~23x) I-HiFi ifunda + 48.39 Gb (~131x ) i-CLR ifundeka + 25 Gb(~69x) NGS + 2 amaseli e-BioNano Irys
I-MH63: 37.88 Gb (~103x) i-HiFi ifunda + 48.97 Gb (~132x) CLR ifundeka + 28 Gb(~76x) NGS + 2 amaseli e-BioNano Irys
Umfanekiso 1 Ama-genome amabili erayisi angenayo igebe (MH63 kanye ne-ZS97)
2ndBanana Genome2
Isihloko: I-Telomere-to-telomere gapless chromosome kabhanana esebenzisa ukulandelana kwe-nanopore
I-Doi:https://doi.org/10.1101/2021.04.16.440017
Isikhathi Sokuthunyelwa: Ephreli 17, 2021.
Isikhungo: Université Paris-Saclay, France
Izinto zokwakha
I-haploid ephindwe kabiliMusa acuminatasppi-malacensis(I-DH-Pahang)
Isu lokulandelanisa nedatha:
Imodi ye-HiSeq2500 PE250 + MinION/ PromethION (93Gb,~200X )+ Imephu yokubuka (DLE-1+BspQ1)
Ithebula 1 Ukuqhathaniswa kwama-genome ase-Musa acuminata (DH-Pahang).
Umfanekiso 2 Musa genomes ukuqhathanisa izakhiwo
3rdI-Phaeodactylum tricornutum genome3
Isihloko: I-Telomere-to-telomere genome assembly ofP
i-haeodactylum tricornutum
I-Doi:https://doi.org/10.1101/2021.05.04.442596
Isikhathi Sokuthunyelwa: May 04, 2021
Isikhungo: Western University, Canada
Izinto zokwakha
I-Phaeodactylum tricornutum(Ukuqoqwa Kwesiko Lolwelwe Ne-Protozoa CCAP 1055/1)
Isu lokulandelanisa nedatha:
1 Oxford Nanopore minion flow cell + a 2×75 paired-end-end outout NextSeq 550 run
Umfanekiso 3 Ukugeleza komsebenzi wokuhlanganiswa kwegenome ye-telomere-to-telomere
4thI-genome yomuntu ye-CHM134
Isihloko: Ukulandelana okuphelele kwe-genome yomuntu
I-Doi:https://doi.org/10.1101/2021.05.26.445798
Isikhathi Sokuthunyelwa: May 27, 2021
Isikhungo: Izikhungo Zezempilo Zikazwelonke (NIH), e-USA
Izinto: umugqa weseli CHM13
Isu lokulandelanisa nedatha:
I-30 × PacBio ukulandelana kwe-circular consensus (HiFi) , 120 × Oxford Nanopore ukulandelana kokufunda okude, 100 × Illumina PCR-Free sequencing (ILMN), 70 × Illumina / Arima Genomics Hi-C (Hi-C), i-Mapsno kanye ne-Strand-seq
Ithebula 2 Ukuqhathaniswa kwe-GRCh38 kanye ne-T2T-CHM13 ye-genome assemblies yabantu
Ireferensi
1.USergey Nurk et al.Ukulandelana okuphelele kwe-genome yomuntu.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798
2.Caroline Belser et al.I-telomere-to-telomere gapless chromosomes yebhanana esebenzisa ukulandelana kwe-nanopore.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017
3.UDaniel J. Giguere et al.I-Telomere-to-telomere genome assembly of Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Ingoma et al.Ukuhlangana Nokuqinisekiswa Kwezinhlobo Ezimbili Zereferensi Ezingenayo I-Gap ze-Xian/indica Rice Yembula Imininingwane Nge-Plant Centromere Architecture.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073
Isikhathi sokuthumela: Jan-06-2022