I-GENOME EVOLUTION
Ukuhlaziya i-genome yokuqhathanisa kugqamisa ukunwetshwa kwegenome ye-transposon-mediated kanye nokwakheka kokuvela kokugoqa kwe-3D genomic kukotini.
Ukulandelana kweNanopore |Hi-C |Ukulandelana kwe-PacBio |Ilumina |Ukulandelana kwe-RNA |I-3D genome architecture |Transposon |I-genomics yokuqhathanisa
Kulolu cwaningo, i-Biomarker Technologies inikeze ukwesekwa kwezobuchwepheshe ekulandeleni kwe-Nanopore, i-Hi-C kanye nokuhlaziywa kwe-bioinformatic efanele.
Abstract
Ukukhulisa i-Transposable element (TE) kuye kwaqashelwa njengamandla ashukumisayo axhumanisa ukunwetshwa kosayizi wofuzo kanye nokuziphendukela kwemvelo, kodwa imiphumela yokulolonga i-3D genomic Architecture ihlala ingaziwa kakhulu ezitshalweni.Lapha, sibika ukuhlangana kwegenome ebangeni lenkomba yezinhlobo ezintathu zikakotini ezisukela ngokuphindwe kathathu ngosayizi wofuzo, okungukuthiI-Gossypium rotundifolium(K2),G. arboreum(A2), kanyeG. raimondii(D5), usebenzisa i-Oxford Nanopore Technologies.I-genome yokuqhathanisa ihlaziya idokhumenti yemininingwane yokukhulisa i-TE eqondene nomugqa onikela kumehluko omkhulu wosayizi we-genome (K2, 2.44 Gb; A2, 1.62 Gb; D5, 750.19 Mb), futhi ibonise okuqukethwe kofuzo okongiwe ngokuqhathaniswa kanye nobudlelwano bokuhlangana phakathi kwezakhi zofuzo.Sithole ukuthi cishe u-17% wezakhi zofuzo ze-syntenic zibonisa ushintsho lwesimo se-chromatin phakathi kwamakhompathimenti asebenzayo (“A”) kanye nangasebenzi (“B”), futhi ukukhulisa i-TE kwakuhlotshaniswa nokwanda kwengxenye yekhompathimenti engu-A ezifundeni zofuzo (~ 7,000 izakhi zofuzo. ) ku-K2 naku-A2 okuhlobene ne-D5.Ama-42% kuphela emingcele yesizinda esihlanganisa i-topologically associating (TAD) aye alondolozwa phakathi kwama-genome amathathu.Idatha yethu ihlanganisa ukukhuliswa kwakamuva kwama-TE kulandela ukwakheka kwemingcele ye-TAD eqondene nomugqa.Lolu cwaningo lunikeza ukukhanya endimeni yokunwetshwa kwe-genome ye-transposon-mediated ekuguqukeni kwesakhiwo se-chromatin esiphezulu ezitshalweni.
Izibalo ezibalulekile zokuhlanganisa i-genome
Umfanekiso.Ukuhlanganiswa kwe-genome nencazelo yesici ye-G. rotundifolium (K2)
Izindaba Namaphuzu Avelele ihlose ukwabelana ngamacala akamuva ayimpumelelo ne-Biomarker Technologies, ithwebula izimpumelelo zesayensi ezinoveli kanye nezindlela ezivelele ezisetshenziswe phakathi nocwaningo.
Isikhathi sokuthumela: Jan-05-2022