I-genotyping ephezulu kakhulu, ikakhulukazi kubantu abaningi, iyisinyathelo esibalulekile ezifundweni zenhlangano yofuzo, ehlinzeka ngesisekelo sofuzo sokutholwa kwezakhi zofuzo ezisebenzayo, ukuhlaziya kokuziphendukela kwemvelo, njll. Esikhundleni sokulandelanisa kabusha kwegenome ejulile, kuncishiswe ukulandelana kofuzo lokumelela(RRGS). ) yethulwa ukuze kuncishiswe izindleko zokulandelanisa isampula ngayinye, kuyilapho kugcinwe ukusebenza kahle okunengqondo ekutholweni komaka wofuzo.Lokhu ngokuvamile kufinyelelwa ngokukhipha isiqeshana esikhawulelwe ngaphakathi kobubanzi obunikeziwe bosayizi, esiqanjwe ngokuthi umtapo wolwazi oncishisiwe (RRL).I-Specific-locus amplified fragment sequencing(SLAF-Seq) isu lokuzithuthukisa lokutholwa kwe-de novo SNP kanye ne-SNP genotyping yabantu abaningi.
Ukuhamba komsebenzi wezobuchwepheshe
I-SLAF vs izindlela ze-RRL ezikhona
Izinzuzo ze-SLAF
Ukusebenza okuphakeme kokuthola umaka wofuzo- Ihlanganiswe nobuchwepheshe bokulandelana komkhiqizo ophezulu, i-SLAF-Seq ingathola amakhulu ezinkulungwane zamathegi atholwe ngaphakathi kofuzo lolo lonke uhlobo lofuzo ukuze kufezwe isicelo samaphrojekthi ocwaningo ahlukahlukene, kungaba nge-genome yethu eyisethenjwa.
Idizayini yokuhlola eyenziwe ngokwezifiso futhi eguquguqukayo- Ngomgomo ohlukile wocwaningo noma izinhlobo, amasu ahlukene okugaya ama-enzymatic ayatholakala kufaka phakathi i-enzyme eyodwa, i-dual-enzyme kanye nokugaya kwama-enzyme amaningi.Isu lokugaya lizohlolwa kusengaphambili ku-silico ukuze kuqinisekiswe idizayini ye-enzyme efanele.
Ukusebenza okuphezulu kwe-enzymatic digestion- Ukugaya okuklanywe kusengaphambili kwe-enzymatic kunikeza ama-SLAF asabalaliswe ngokulinganayo ku-chromosome.Ukuqoqwa kweziqephu ngendlela efanele kungazuza ngaphezu kwama-95%.
Gwema ukulandelana okuphindaphindayo– Iphesenti lokulandelana okuphindaphindiwe kudatha ye-SLAF-Seq yehliswa ukuze ibe ngaphansi kuka-5%, ikakhulukazi ezinhlotsheni ezinezinga eliphezulu lezinto eziphindaphindayo, ezifana nokolweni, ummbila, njll.
Ukuzithuthukisa kwe-bioinformamatic workflow- I-BMK ithuthukise ukuhamba komsebenzi okudidiyelwe kwe-bioinformamatic okusebenzayo kubuchwepheshe be-SLAF-Seq ukuze kuqinisekiswe ukwethembeka nokunemba kokuphumayo kokugcina.
Ukusetshenziswa kwe-SLAF
Imephu yokuxhumanisa yofuzo
Ukwakhiwa kwemephu yofuzo enokuminyana okuphezulu kanye nokuhlonzwa kwe-loci elawula izici zohlobo lwezimbali e-Chrysanthemum(Chrysanthemum x morifolium Ramat.)
Ijenali: Ucwaningo Lwezokulima Ishicilelwe: 2020.7
I-GWAS
Ukuhlonzwa kofuzo lwekhandidethi elihlotshaniswa nokuqukethwe kwe-isofavone kumbewu kabhontshisi wesoya kusetshenziswa i-genome-wide association kanye nokuxhumanisa imephu
Ijenali: Ijenali Yezitshalo Ishicilelwe: 2020.08
I-Evolutionary Genetics
Ukuhlaziywa kwe-genomic yabantu kanye nomhlangano we-de novo kuveza umsuka werayisi elinokhula njengomdlalo wokuziphendukela kwemvelo
Ijenali: Isitshalo Samangqamuzana Ishicilelwe: 2019.5
I-Bulked Segregant Analysis (BSA)
I-GmST1, efaka i-sulfotransferase, inikeza ukumelana nezinhlobo zegciwane le-soybean mosaic i-G2 ne-G3.
Ijenali: Isitshalo, Iseli&Imvelo Ishicilelwe: 2021.04
Ireferensi
Sun X, Liu D, Zhang X, et al.I-SLAF-Seq: indlela ephumelelayo yokutholwa kwe-SNP yezinga elikhulu ne-genotyping kusetshenziswa ukulandelana komphumela ophezulu[J].I-Plos one, 2013, 8(3):e58700
Ingoma X, Xu Y, Gao K, et al.Ukwakhiwa kwemephu yofuzo enokuminyana okuphezulu kanye nokuhlonzwa kwe-loci elawula izici zohlobo lwezimbali ku-Chrysanthemum (Chrysanthemum × morifolium Ramat.).I-Hortic Res.2020;7:108.
Wu D, Li D, Zhao X, et al.Ukuhlonzwa kofuzo lwekhandidethi elihlotshaniswa nokuqukethwe kwe-isoflavone kumbewu kabhontshisi wesoya kusetshenziswa i-genome-wide association kanye nokuxhumanisa imephu.Isitshalo J. 2020;104(4): 950-963.
Sun J, Ma D, Tang L, et al.I-Population Genomic Analysis kanye ne-De Novo Assembly Yembula Imvelaphi Ye-Weedy Rice njengomdlalo wokuziphendukela kwemvelo.I-Mol Plant.2019;12(5):632-647.I-Mol Plant.2018;11(11):1360-1376.
Zhao X, Jing Y, Luo Z, et al.I-GmST1, efaka i-sulfotransferase, inikeza ukumelana nezinhlobo zegciwane le-soybean mosaic i-G2 ne-G3.Plant Cell Environ.2021;10.1111/pce.14066
Isikhathi sokuthumela: Jan-04-2022