IMICROBIAL ECOLOGY
Ukuqiniswa kwezolimo kunciphisa ubunkimbinkimbi benethiwekhi ye-microbial kanye nobuningi be-keystone taxa ezimpandeni
I-Amplicon Sequencing yobude obugcwele (ITS) |I-PacBio HIFI
Amaphuzu avelele
Aukuqina kwezolimo sekuya ngokuya kuba yinkinga ngenxa yemiphumela yako emibi yezemvelo okuhlanganisa ukungasebenzisi kahle kwezakhi zomzimba, ukuthuthwa kwamanzi angaphansi komhlaba, ukuwohloka kwekhwalithi yenhlabathi, njll. Ezinye izinhlelo zokulima ezihlanganisa ukungalimi nokulima ngemvelo ziye zamukelwa kabanzi ukuze kuncishiswe umonakalo.Umphakathi we-Microbial udlala indima ebalulekile ekukhiqizeni nasekusimameni kwe-agroecosystem.Kodwa-ke, akucaci ukuthi izinhlelo zokulima ezihlukene ziyithinta kanjani i-root microbiota.
Idizayini Yokuhlola
Izivivinyo
Samasampula kawoyela nezimpande(DNA) ayevela emasimini kakolweni avela emapulazini angama-60 (angama-20 lilinye)
Gukuzulazula: 1. Convention (nokulima);2. Ingqungquthela (akulinywa);3. Ipulazi lemvelo
Sisu lokulinganisa: Ukulandelana kwe-amplicon yobude obugcwele (ITS)
Pama-rimers: ITS1F-ITS4 (iqondise sonke isifunda se-ITS ~630 bp)
Sinkundla yokulinganisa: PacBio RS II
Ukuhlaziywa kwe-Bioinformatics
Imiphumela
On isilinganiso sama-OTU angama-357 akhonjwe indawo ngayinye kanye nesamba sama-OTU angama-837 kuzo zonke iziza ezingama-60.Ukuhlukahluka kwe-alpha kwemiphakathi yezimpande zesikhunta bekungabonisi umehluko omkhulu phakathi kwezinhlelo ezintathu zokulima.Kodwa-ke, amaqoqo amathathu ahlukene akhiwa ekuhlaziyeni ukuhlukahluka kwe-beta, okubonisa imiphumela enamandla yohlelo lokulima esakhiweni somphakathi wesikhunta.
Umfanekiso 1. Ukwehlukahlukana kwe-Alpha (Inkomba ye-Shannon nokwakheka komphakathi) kanye nokuhlaziywa kokuhlukahluka kwe-beta (ukuhlaziywa kwe-canonical yezixhumanisi eziyinhloko) emiphakathini yesikhunta esiyizimpande.
Ten i-keystone taxa yachazwa ngokususelwa kunethiwekhi iyonke ye-fungal imiphakathi ohlelweni lokulima oluthathu: izindawo eziphezulu eziyi-10 ezineziqu eziphakeme kakhulu, ukusondelana okuphezulu kakhulu kanye nendawo ephakathi nendawo ephansi kakhulu yakhethwa.Eziyisikhombisa zazo zazingezalezo ze-mycorrhizal.
Umfanekiso 2. Inethiwekhi iyonke emiphakathini yezimpande zefungal yezinhlelo ezintathu zokulima
Famanethiwekhi athile esistimu yezikhali abonise ukuxhumana okuphezulu kakhulu kunethiwekhi ye-organic enemiphetho ephindwe kabili kanye nama-node axhumeke kakhulu kunenethiwekhi ye-no-till kanye nenethiwekhi evamile.Ngaphezu kwalokho, inethiwekhi yokulima okuphilayo ibambe intela yetshe eliyisihluthulelo ethe xaxa (idayimane) uma iqhathaniswa nayo yonke enye, eyayisekela ubunkimbinkimbi bayo nokuxhumana.
Umfanekiso 3. Uhlelo lokulima oluqondene nezimpande ze-fungal networks
AUkuhlangana okunamandla okungekuhle phakathi kokuqina kwezolimo kanye nokuxhumana kwenethiwekhi yesikhunta kwabonwa.Ukuhlaziywa kwehlathi okungahleliwe kwembule abashayeli abayinhloko bentela yetshe elingukhiye: i-phosphorus yenhlabathi, ukuminyana kwenqwaba, i-pH kanye ne-mycorrhizal colonization.
Umfanekiso 4. Ukuqina kwezolimo kanye nokuxhumana kwenethiwekhi kuzo zonke izinhlelo zokulima ezintathu (A no-B);Ukuhlaziywa kwehlathi okungahleliwe(C) kanye nobudlelwano phakathi kokuqina kwezolimo kanye nekoloni ye-AMF (D)
Ubuchwepheshe
Ukulandelana kwe-amplicon yobude obugcwele
A"Ukulandelana Kwesizukulwane Sesithathu" kungena esiteji, imikhawulo ezindaweni ezihlosiwe kanye nezinkinga ekuhlanganiseni kwe-de novo kunqotshiwe.I-Pacific Bioscience (i-PacBio) ikhulise ngempumelelo ukufundwa kokulandelana kumashumi ama-kilobase, okusivumela ukuthi sithole ukufundwa kobude obugcwele be-16s rRNA (1,000 bp-1,500 bp) kumabhaktheriya noma i-18S rRNA (1,500 bp-2,000 bp) kanye ne-ITS izifunda (400 bp-900 bp) kuma-eukaryotic.Ukubuka okubanzi komkhakha wezofuzo kwathuthukisa kakhulu ukuxazululwa kwencazelo yezinhlobo nezakhi zofuzo ezisebenzayo.Udaba olude olukhathazayo mayelana nokunemba kwesisekelo luxazululwe ngokuzilungisa kwe-PacBio CCS, okukhiqiza ukufundwa kwe-HIFI ngokunemba okungaphezu kuka-99%.
Ukusebenza kusichasiselo se-OTU
Tngokuthola izinzuzo zakho kokubili ukufundwa okude nokudluliswa okuphezulu, ukunemba kwesichasiselo kungakhuliswa kakhulu futhi kuzuzwe ukulungiswa "kwezinga lezinhlobo zezilwane" ekuhlonzeni ama-microbial.
Ireferensi
Banerjee, Samiran, et al."Ukuqiniswa kwezolimo kunciphisa ubunkimbinkimbi benethiwekhi ye-microbial kanye nensada ye-keystone taxa ezimpandeni."Ijenali ye-ISME (2019).
Ubuchwepheshe Nokugqama ihlose ukwabelana ngohlelo lokusebenza oluyimpumelelo lwakamuva kakhulu lobuchwepheshe bokulandelana komphumela ophezulu ezinkundleni ezihlukahlukene zokucwaninga kanye nemibono ekhaliphile ekwakhiweni kokuhlola kanye nokumbiwa kwedatha.
Isikhathi sokuthumela: Jan-08-2022