Ukuhlola amaphethini wokuvezwa kwezakhi zofuzo endaweni yokuqala yento ephilayo kubalulekile ekuqondeni izinhlobo nemisebenzi yamaseli ayo.Kodwa-ke, izindlela zamanje zokuhlaziya i-spapatial transcriptome zinemikhawulo njengokusebenzisa okuphansi noma ukulungiswa okwanele.I-BMKMANU S1000 Spatial Chip, ethuthukiswe yi-BMKMANU, ingathola ulwazi lwe-in-situ gene expression ezigabeni eziphelele zethishu ekulungisweni kwamangqamuzana angaphansi, okuvumela ukutolika okuhlukene kwesakhiwo sezicubu.
Lungela ukuzwa umkhiqizo wethu omusha!Ukuze sigubhe ukukhishwa kwayo, sinikezela ngesipesheliThenga Okune Thola Okukodwa Mahhalaiphromoshini yesikhathi esilinganiselwe kuphela.
Izingxenye Zomkhiqizo
I-BMKMANU S1000 chip yendawo kanye nekhithi ye-reagent efanayo ihlanganisa:
1) Ikhithi Yokuthuthukisa Izicubu, equkethe ama-reagents okuthuthukisa izicubu (ukuhlola isikhathi esilungile sokwenza izicubu zikwazi ukungena).
2) Ikhithi ye-Gene Expression, equkethe ama-reagents okuthwebula i-mRNA ezincekwini zethishu ukuze ilungiselelwe nokulandelanisa umtapo wolwazi.
3) Ikhithi Yokuqalisa: Ihlanganisa i-adaptha ye-thermostatic kanye ne-transcriptome magnetic separator.
Isimiso Sobuchwepheshe
I-chip isekelwe kuma-micropores nama-microbeads, futhi i-mRNA ingashuthwa endaweni nge-Oligo kuma-microbeads.I-Oligo yakhiwe izingxenye ezine: Read1, Spatial Barcode, UMI, and Poly(dT)VN.Ngemuva kokuthi izicubu zinamathiselwe ku-chip, i-mRNA ikhishwa esicutshini nge-enzyme ye-permeabilization.Njengoba iziphetho eziningi ze-mRNA 3' zinomsila we-Poly-A, zithwetshulwa i-Oligo nge-Poly(dT)VN.Ngemva kokukhulisa i-RT-PCR ne-cDNA, ukulungiswa komtapo wolwazi nokulandelana kwawo, ukulandelelwa kwendawo kufinyelelwa ngeBhakhodi Yendawo.Lokhu kuvumela ukuhlaziywa kwesisho sofuzo ezindaweni zendawo kanye nokuhlukahluka kwezicubu.
Izinzuzo Zomkhiqizo
Izinhlelo zokusebenza
Ingasetshenziswa ezindaweni eziningi zocwaningo lwebhayoloji nolwezokwelapha, okuhlanganisa isimila, izifo, i-immunology, kanye nesayensi yokuthuthuka, esiza ukuzuza impumelelo entsha kule mikhakha ngokuhlaziywa kwesimo sendawo ekuxazululweni kwe-subcellular.
●Isimila Nezifo:
I-Spatial heterogeneity kanye ne-microenvironment yamathumba nezifo
Ukuqala nokuthuthukiswa kwezimila nezifo
Impendulo yokwelashwa kwezimila nezifo
●Developmental Biology
I-atlas ye-Spatiotemporal yezitho
Izindlela zokulawula izakhi zofuzo ngesikhathi sokuthuthukiswa
●Ukusabela Ngokucindezeleka
Impendulo ye-Biotic stress
Impendulo ye-Abiotic stress
●I-Immunology
Ukusabela kwamasosha omzimba ekushintsheni izitho zomzimba
Izindlela zokuzivikela ezifweni zezimila nezifo
I-Pathogenesis yezifo ze-autoimmune
●Ukuhlaziywa kokumelana nezidakamizwa
Izindlela zokumelana nezidakamizwa
Ucwaningo nokuthuthukiswa kwemithi emisha
Amacala kanye nedatha yedemo
I-BMKMANU S1000 iqinisekiswe ukusebenza kumakhulu ezimo ezinhlotsheni zezicubu ezihlukene.
