-
Ukulandelanisa Kwezitshalo/Izilwane ze-De novo Genome
De novoukulandelana kubhekisela ekwakhiweni kwegenome yohlobo lonke kusetshenziswa ubuchwepheshe bokulandelana, isb. PacBio, Nanopore, NGS, njll., ngaphandle kofuzo oluyisethenjwa.Ukuthuthukiswa okuphawulekayo kobude bokufunda kobuchwepheshe bokulandelana kwesizukulwane sesithathu kulethe amathuba amasha ekuhlanganiseni ama-genome ayinkimbinkimbi, njengalawo ane-heterozygosity ephezulu, isilinganiso esiphezulu sezifunda eziphindaphindayo, ama-polyploid, njll. Ngobude bokufunda ezingeni lamashumi ama-kilobase, lokhu kufunda kokulandelana kwenza kube lula. ukuxazulula izici eziphindaphindayo, izifunda ezinokuqukethwe kwe-GC okungajwayelekile nezinye izifunda eziyinkimbinkimbi kakhulu.
I-Platform: I-PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq6000
-
I-Hi-C based Genome Assembly
I-Hi-C iyindlela edizayinelwe ukuthwebula ukulungiselelwa kwekhromozomi ngokuhlanganisa ukuhlola okusekwe ekusondeleni kanye nokulandelana komphumela ophezulu.Amandla alokhu kusebenzisana kukholakala ukuthi ahlotshaniswa kabi nebanga elingokomzimba kuma-chromosome.Ngakho-ke, idatha ye-Hi-C ingaqondisa ukuhlanganisa, ukuhleleka nokuqondiswa kokulandelana okuhlanganisiwe ku-genome esalungiswa futhi iqinise lezo enanini elithile lama-chromosome.Lobu buchwepheshe bunika amandla ukuhlangana kweleveli ye-chromosome yofuzo ngaphandle kwemephu yofuzo esekelwe kubantu.Yonke i-genome eyodwa idinga i-Hi-C.
Ipulatifomu: Illumina NovaSeq6000 / DNBSEQ
-
I-Evolutionary Genetics
I-Evolutionary genetics iyisevisi egcwele yokulandelana eyenzelwe ukuhlinzeka ngencazelo ebanzi yolwazi lokuziphendukela kwemvelo lwezinto ezinikeziwe ngokusekelwe ezinhlobonhlobo zofuzo, okuhlanganisa ama-SNP, ama-InDels, ama-SV nama-CNV.Ihlinzeka ngakho konke ukuhlaziya okuyisisekelo okudingekayo ekuchazeni izinguquko zokuziphendukela kwemvelo nezici zofuzo zabantu, njengesakhiwo sabantu, ukuhlukahluka kofuzo, ubudlelwano be-phylogeny, njll. Futhi iqukethe izifundo ngokugeleza kwezakhi zofuzo, okunika amandla ukulinganiswa kobukhulu besibalo esisebenzayo, isikhathi sokuhlukana.
-
I-Genomics yokuqhathanisa
I-genomics yokuqhathanisa ngokwezwi nezwi isho ukuqhathanisa ukulandelana okuphelele kwe-genome nezakhiwo zezinhlobo ezahlukene.Lesi sinqumo sihlose ukuveza ukuvela kwezinhlobo zezinto eziphilayo, ukusebenza kwezakhi zofuzo, indlela yokulawula izakhi zofuzo ezingeni le-genome ngokuhlonza izakhiwo zokulandelana nezici ezilondolozwe noma ezehlukanisa kuzo zonke izinhlobo zezinto eziphilayo.Ucwaningo olujwayelekile lokuqhathanisa lwe-genomics luhlanganisa ukuhlaziya emndenini wezakhi, ukukhula kokuziphendukela kwemvelo, ukuphindaphinda kofuzo lonke, ingcindezi ekhethiwe, njll.
-
Ukuhlaziywa kwe-Bulked Segregant
Ukuhlaziywa kwe-bulked segregant (BSA) kuyindlela esetshenziswayo ukuhlonza ngokushesha izimpawu zofuzo ezihlotshaniswa ne-phenotype.Ukuhamba komsebenzi okuyinhloko kwe-BSA kuqukethe ukukhetha kwamaqembu amabili abantu abane-phenotypes ephikisana kakhulu, okuhlanganisa i-DNA yabo bonke abantu ukuze bakhe inqwaba ye-DNA, okukhomba ukulandelana okuhlukene phakathi kwamachibi amabili.Le nqubo isetshenziswe kakhulu ekuhlonzeni izimpawu zofuzo ezihlotshaniswa kakhulu nezakhi zofuzo ezihlosiwe ku-genome yezitshalo/yezilwane.
