Ukuhlaziywa kwe-Eukaryotic mRNA (ngereferensi)
Ngokusekelwe ekulandeleni kofuzo okwaziwayo nolwazi lwesichasiselo, isizukulwane esisha sedatha yokulandelana kokuphuma okuphezulu (i-RNA-Seq) sisetshenziswa njengokufakwayo, futhi amasayithi amasha okuloba (ufuzo olusha) kanye nemicimbi emisha yokuhlanganisa okuhlukile kuyahlonzwa.ukulandelana kokuhlolwa kwekhwalithi yedatha;ukulandelana kwedatha nokuhlelwa kokulandelana kwegenome yereferensi ekhethiwe, ukukhomba imingcele ye-exon / intron, ukuhlaziya ukwahluka kwezakhi zofuzo, ukuhlola izifunda zofuzo nokulotshiwe okusha, ukuhlonza amasayithi e-SNP esifunda esilotshiwe, umngcele phakathi kuka-3'no-5 ' izakhi zofuzo, kanye nesichasiselo esisebenzayo nokuhlaziya okunothisayo kwamasampuli ahlukene (amaqembu).
Ama-RNA amade angafaki ikhodi
Ama-RNA amade angafaki amakhodi (lncRNA) awuhlobo lwemibhalo enobude obude kuno-200 nt, engakwazi ukufaka ikhodi amaprotheni.Ubufakazi obuningi buphakamisa ukuthi iningi lama-lncRNA kungenzeka ukuthi asebenze.Ubuchwepheshe bokulandelana komkhiqizo ophezulu kanye namathuluzi okuhlaziya nge-bioinformatic kusinikeza amandla okuveza ukulandelana kwe-lncRNA nolwazi lokubeka kahle kakhudlwana futhi kusiholele ekutholeni ama-lncRNA anemisebenzi ebalulekile yokulawula.I-BMKCloud iyaziqhenya ngokunikeza amakhasimende ethu inkundla yokuhlaziya ukulandelana kwe-lncRNA ukuze afinyelele ukuhlaziya kwe-lncRNA okusheshayo, okuthembekile nokuguquguqukayo.
16S/18S/ITS amplicon ukulandelana
Inkundla yokuhlaziya ukwehlukahlukana kwamagciwane ithuthukiswa ngeminyaka yokuhlangenwe nakho kokuhlaziywa kwephrojekthi ye-microbial diversity, equkethe ukuhlaziya okuyisisekelo okujwayelekile kanye nokuhlaziya komuntu siqu: ukuhlaziya okuyisisekelo kuhlanganisa okuqukethwe kokuhlaziya okujwayelekile kocwaningo lwamanje lwe-microbial, okuqukethwe kokuhlaziya kucebile futhi kubanzi, bese kwethulwa imiphumela yokuhlaziya. ngendlela yemibiko yephrojekthi;Okuqukethwe kokuhlaziywa komuntu siqu kuhlukahlukene.Amasampuli angakhethwa futhi amapharamitha angasethwa ngendlela evumelana nezimo ngokuvumelana nombiko wokuhlaziya oyisisekelo kanye nenjongo yocwaningo, ukuze kufezeke izidingo eziqondene nawe.Isistimu yokusebenza yeWindows, elula futhi esheshayo.
I-Shotgun Metagenomics(NGS)
Idatha ye-Metagenomic isetshenziselwa ukuhlaziya izinto ezixubile ze-genomic ezikhishwe kumasampula emvelo, okunikeza ulwazi oluningiliziwe ngokuhlukahluka kwezinhlobo zezilwane nobuningi, ukwakheka kwabantu, ubudlelwano be-phylogenetic, izakhi zofuzo ezisebenzayo, kanye nenethiwekhi yokuxhumana nezici zemvelo.
I-NGS-WGS(Illumina/BGI)
Ipayipi elihlanganisiwe lokuhlaziya elenzelwe ochwepheshe bocwaningo ngaphandle kolwazi lwangaphambili lwe-bioinformatics futhi lisebenza kuseva esebenza kahle kakhulu.Isiza ukuqedwa ngokushesha kwemisebenzi efana nokulawula ikhwalithi yedatha, ukuqondanisa ukulandelana, ukutholwa kokuhlukahluka kwe-SNP/InDel/SV, isichasiselo, nofuzo lokushintsha.
I-GWAS
Kusetshenziswa izindlela ezithile zezibalo, ukuhlaziya kwe-GWAS kuhlose ukwembula ukuhlukahluka kwe-nucleotide ebanzi yegenome ehlotshaniswa nomehluko we-phenotypic.Idlala indima ebalulekile ekuhloleni izakhi zofuzo ezisebenzayo ezihlobene nezifo eziyinkimbinkimbi zabantu kanye nezici eziyinkimbinkimbi ezitshalweni nasezilwaneni.
BSA
Inkundla yokuhlaziya edidiyelwe ihlinzeka ngesixhumi esibonakalayo esisebenziseka kalula sokuhlaziya ukwehlukahlukana komuntu siqu okulinganiselwe.Ukuhlaziywa kwe-BSA kuhilela ukuhlanganisa abantu abanezimpawu ezeqisayo ze-phenotypic kusuka kubantu abahlukanisayo.Ngokuqhathanisa i-loci ehlukile phakathi kwamasampuli ahlanganisiwe, le ndlela isheshe ihlonze izimpawu zamangqamuzana ezixhumene eduze nezakhi zofuzo eziqondiwe.Isetshenziswa kakhulu ekwenzeni imephu yofuzo yezitshalo nezilwane, iyithuluzi elibalulekile lokuzalanisa okusizwa umaka kanye nokubeka izakhi zofuzo.
I-Evolutionary Genetics
Ukuhamba komsebenzi wokuhlaziya okudidiyelwe okuklanyelwe ochwepheshe bocwaningo abanolwazi olulinganiselwe lwe-bioinformatics.Isebenzisa isipiliyoni esibanzi se-BMKGENE kumaphrojekthi wokuguquguquka kofuzo, le nkundla isebenza kumaseva asebenza kahle kakhulu, iqinisekisa ukukhishwa okusheshayo nokunembayo kokuhlaziya komuntu siqu.Lokhu kuhlanganisa imisebenzi efana nokwakhiwa kwesihlahla se-phylogenetic, ukuhlaziywa kokungalingani, ukuhlola ukuhlukahluka kofuzo, ukuhlonza okukhethiwe kokushanela, ukuhlaziya ukuhlobana, ukuhlaziywa kwengxenye eyinhloko, kanye nokuhlukaniswa kwesakhiwo sabantu.
