Gxila emandleni okutadisha izinhlobonhlobo zasendle zezinhlobo zocwaningo, eziboniswa yindaba ebaluleke kakhulu evela kumakhasimende e-BMKGENE.Esanda kushicilelwa kumagazini walo nyaka we-Plant Biotechnology Journal, isihloko esithi “Chromosome-level Wild Hevea brasiliensis Genome: Empowering Genomic-Assisted Breeding and Unearthing Vital Loci for Elevated Rubber Yield” simi njengesithenjwa esibalulekile.
Isihlahla senjoloba sase-Brazilian (Hevea brasiliensis) simi side njengomthombo wenjoloba wemvelo obalulekile.Kuwo wonke ikhulunyaka elidlule, izindlela zokuzalanisa zendabuko zabangela ukwanda okuphindwe kasithupha kokukhiqizwa kwenjoloba.Nokho, isisekelo sofuzo esinesibopho sokuthuthukisa isivuno senjoloba esingaba khona sihlala sixakile.
Lolu cwaningo luqale ukwakha i-genome yezinga le-chromosome yesihlahla senjoloba yasendle, sisebenzisa ukulandelana kwe-Nanopore okufunde isikhathi eside, ukulandelana kwe-Illumina NGS, kanye nobuchwepheshe be-Hi-C.
Ngokuhambisanayo, i-trove yezinsiza ze-germplasm engu-147, ehlanganisa ukuhlukahluka kwesivuno esikhulu, izinhlobo zasendle, nezinhlobo ezihlobene nayo, yahlanganiswa ukuze kuhlangatshezwane nayo yonke i-genome resequencing (WGS) kusetshenziswa iplathifomu ye-Illumina.Idatha yokulandelana eyalandela iye yahlaziywa ngokuqinile izakhi zofuzo zabantu, okuvumela ukuhlukaniswa kwesakhiwo sabantu, ukuhlukahluka, nokungalingani kokuxhumaniseka.Lokhu kuhlaziya kunikeza ukukhanya ezinhlobonhlobo zokufuywa kuzo zonke izizwe.
Uphenyo olwengeziwe luhlanganisa ukuhlaziya okuhlanganisa i-Fst, π, Tajima's D, kanye ne-LD, okuhloswe ngayo ukwembula amasiginali okukhetha avela ekuvuthweni kwe-latex okuthuthukisiwe, okugxile kakhulu kuma-clones e-Wickham.
Ukufika kuvuthondaba Ekuhlaziyweni Kwenhlangano Yomhlaba Wonke (GWAS), omaka abaxhunywe nezici zesivuno kanye nofuzo oluhlobene nesivuno kamuva bakhonjwa.Ukuqinisekisa kwasekuqaleni kwembule imisebenzi ehlukile yokulotshiweyo kuzo zonke izinhlobo zofuzo ezihlukile ze-ERF, okunikeza umbono wokuqala wokubaluleka kokusebenza kwawo.Okunye ukuqinisekiswa kuhlelelwe ocwaningweni oluzayo.
I-BMKGENE iyaziqhenya ngeqhaza ekulandeleni okubanzi kofuzo kwalesi sibalo sabantu abaningi, kanye neqhaza elikhulu ekuhlaziyweni kwe-bioinformatics.Umuzwa wethu omkhulu uhlanganisa ukulandelana kwe-NGS/TGS kanye nokuhlaziywa kwe-multiomics bioinformatics kuzo zonke izinhlobo zezinhlobo eziyi-1000+.Sibheke ngabomvu ithemba lokubambisana nawe kumaphrojekthi akho azayo.
Chofoza lapha ukuzefunda kabanzi ngalolu cwaningo
Isikhathi sokuthumela: Aug-29-2023