I-BMKGENE iyaziqhenya ukumemezela ukutholakala kwe-PacBio yokulandelanisa ethuthukisiwe, i-Revio, esisiza ukuthi silethe umphumela ophakeme kanye nezinsizakalo zokulandelanisa ezifunde isikhathi eside ezisezingeni eliphakeme kubacwaningi.
*Kungani Khetha i-PacBio Revio?
*Izicelo ze-PacBio Revio
•Ukulandelana kwe-genome
1.De Novoukuhlanganiswa kwe-genome- I-Revio isiza ekuhleleni nasekuhlanganisweni kwezakhi zofuzo ezinkulu kakhulu neziyinkimbinkimbi, ihlinzeka ngemininingwane ebucayi ngokuhlukahluka kofuzo, ukwakheka kwezakhi zofuzo, kanye nomsebenzi.
2.T2T genome- I-Revio, ehlanganiswe namasu afana ne-Hi-C, ingathuthukisa ikhwalithi yama-genomes avamile futhi isize izinhlobo eziningi zofuzo zithole i-genome yereferensi yezinga le-T2T, okwenza abacwaningi baqonde kangcono ukuhlukahluka kofuzo phakathi kwabantu naphakathi kwabantu.
3.Ukulandelana kofuzo lonke- I-Revio inganikeza imininingwane ebanzi ngesakhiwo kanye nokusebenza kwawo wonke ama-genomes, okuhlanganisa i-SNP, i-Indel, i-SV, i-CNV, njll., ikakhulukazi kubantu abaningi bofuzo.
4.Ukulandelana kwe-pan-genome- I-Revio ilungele ukulandelana ngendlela ephumelelayo nangokungabizi kanye nokuhlanganisa amagenome amaningi ohlobo oluthile ngesikhathi esisodwa ukuze kwakhiwe i-pan-genome, ehlinzeka ngemininingwane ebalulekile ekuguquguqukeni kwe-genomic nokuziphendukela kwemvelo.
•Ukulandelana kwe-Transcriptome
1.Ukulandelana kokulotshiweyo kobude obugcwele- Ngobude bayo obude bokufunda, i-Revio inika amandla ukulandelana kombhalo wobude obugcwele bekhwalithi ephezulu, ihlinzeka ngemininingwane ebanzi kokunye ukuhlanganisa, ukuhlanganisa izakhi zofuzo, kanye nesisho se-isoform.
2.Iphrofayili yobude obugcwele yamasampula amakhulu- I-Revio inganikeza ukulinganisa okunembe kakhulu kwamaleveli enkulumo yofuzo kuwo wonke amasampula esilinganiso esikhulu, inike amandla imininingwane ebalulekile ekulawuleni izakhi zofuzo nokuhlukaniswa kweseli.
3.Ukulandelana kwe-transcriptome yeseli elilodwa- I-Revio inganikeza idatha yombhalo wombhalo weseli elilodwa enesinqumo esiphezulu, ivumele abacwaningi ukuthi bahlole ukuhlukahluka kwamaselula futhi bahlonze izinhlobo zamaseli ezingavamile.
•Ukulandelana kwe-Microbial
1.16S ukulandelana kobude obugcwele- I-Revio inika amandla ukulandelana kwekhwalithi ephezulu kwe-16S rRNA, ihlinzeka ngemininingwane enemininingwane ekwakhiweni nasekusebenzeni komphakathi we-microbial.
2.Ukulandelana kwe-amplicon efundwe isikhathi eside- I-Revio ifaneleka kahle ukulandelana kwe-amplicon okufundwe isikhathi eside, okwenza abacwaningi bafunde ukuhlukahluka kwamagciwane kuma-ecosystem ayinkimbinkimbi.