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条形 isibhengezo-03

Imikhiqizo

I-Evolutionary Genetics

I-Evolutionary genetics iyisevisi egcwele yokulandelana eyenzelwe ukuhlinzeka ngencazelo ebanzi yolwazi lokuziphendukela kwemvelo lwezinto ezinikeziwe ngokusekelwe ezinhlobonhlobo zofuzo, okuhlanganisa ama-SNP, ama-InDels, ama-SV nama-CNV.Ihlinzeka ngakho konke ukuhlaziya okuyisisekelo okudingekayo ekuchazeni izinguquko zokuziphendukela kwemvelo kanye nezici zofuzo zabantu, njengesakhiwo sabantu, ukuhlukahluka kofuzo, ubudlelwano be-phylogeny, njll. Futhi iqukethe izifundo ngokugeleza kwezakhi zofuzo, okunika amandla ukulinganiswa kobukhulu besibalo esisebenzayo, isikhathi sokuhlukana.


Imininingwane Yesevisi

Imiphumela yedemo

Ucwaningo lwesigameko

Izinzuzo Zesevisi

1Izakhi zofuzo zokuziphendukela kwemvelo

Takagi et al.,Ijenali yezitshalo, 2013

● Ukulinganisa isikhathi sokuhlukana kwezinhlobo zezilwane nesivinini ngokusekelwe ezinhlobonhlobo zeleveli yama-nucleotide nama-amino acid.
● Ukwembulwa kobudlelwane obuthembeke kakhulu be-phylogenetic phakathi kwezinhlobo zezilwane ezinethonya elincishisiwe lokuguquguquka okuguquguqukayo kanye nokuziphendukela kwemvelo okufanayo
● Ukwakha izixhumanisi phakathi kwezinguquko zofuzo nama-phenotypes ukuze kwambulwe izakhi zofuzo ezihlobene nezici
● Ukulinganisa ukuhlukahluka kwezakhi zofuzo, okubonisa amandla okuziphendukela kwemvelo kwezinhlobo zezinto eziphilayo
● Isikhathi sokushintsha esisheshayo
● Okuhlangenwe nakho okubanzi: I-BMK iqongelele ulwazi olukhulu ngenani labantu kanye namaphrojekthi ahlobene nokuziphendukela kwemvelo iminyaka engaphezu kwengu-12, ehlanganisa amakhulu ezinhlobo zezilwane, njll. futhi yanikela kumaphrojekthi wezinga eliphezulu angaphezu kuka-80 ashicilelwe ku-Nature Communications, Izitshalo Zemolekyuli, I-Plant Biotechnology Journal, njll.

Imininingwane Yesevisi

Izinto zokwakha:

Ngokujwayelekile, kunconywa okungenani izigaba ezintathu ezincane (isib. izinhlobonhlobo noma izinhlobo).Inani elincane ngalinye kufanele libe nabantu abangekho ngaphansi kuka-10 (Izitshalo >15, zingancishiselwa izinhlobo ezingavamile).

Isu lokulandelanisa:

* I-WGS ingaqashwa ezinhlotsheni ezine-genome yereferensi yekhwalithi ephezulu, kuyilapho i-SLAF-Seq isebenza ezinhlotsheni ezine-genome eyisithenjwa noma ezingenayo, noma i-genome eyireferensi yekhwalithi embi.

Isebenza kusayizi wofuzo

WGS

Amathegi e-SLAF (×10,000)

≤ 500 Mb

10×/ngamunye

I-WGS inconywa kakhulu

500 Mb - 1 Gb

10

1 Gb - 2 Gb

20

≥2 Gb

30

I-Bioinformatics ihlaziya

● Ukuhlaziya kokuziphendukela kwemvelo

● Ukushanela okukhethekile

● Ukugeleza kofuzo

● Umlando wezibalo zabantu

● Isikhathi sokuhlukana

i-evolution 2

Izidingo Zesampula Nokulethwa

Izimfuneko zesampula:

 

Izinhlobo

 Izicubu

I-WGS-NGS

I-SLAF

Isilwane

 

  

Izicubu ze-Visceral

 

0.5~1g

 

 

0.5g

 

 

 Izicubu zemisipha

Igazi lezilwane ezincelisayo

 

1.5mL

 

 

1.5mL

 

Igazi lezinkukhu/Inhlanzi

Isitshalo

  

  Iqabunga Elisha    

1~2g

   

0.5~1g

 I-Petal/Isiqu
  Impande/Imbewu
 

Amaseli

  Iseli Cultured    

 

gDNA

Ukugxila
(ng/ul)

Inani

(ug)

OD260/OD280

I-SLAF

≥35

≥1.6

1.6-2.5

I-WGS-NGS

≥1

≥0.1

-

Ukugeleza Komsebenzi Wesevisi

Isampula ye-QC

Idizayini yokuhlola

ukulethwa kwesampula

Ukulethwa kwesampula

Ukulungiselela Umtapowolwazi

Ukwakhiwa komtapo wolwazi

Ukulandelanisa

Ukulandelanisa

Ukuhlaziywa kwedatha

Ukuhlaziywa kwedatha

Ngemva kokudayiswa Services

Amasevisi angemuva kokuthengisa


  • Okwedlule:
  • Olandelayo:

  • *Imiphumela yedemo eboniswe lapha yonke ivela kuzakhi zofuzo ezishicilelwe nge-BMKGENE

    Ukuhlaziywa kwe-1.Evolution kuqukethe ukwakhiwa kwesihlahla se-phylogenetic, isakhiwo sabantu kanye ne-PCA ngokusekelwe ezinhlobonhlobo zofuzo.

    Isihlahla se-Phylogenetic simele ubudlelwano be-taxonomic kanye nokuziphendukela kwemvelo phakathi kwezinhlobo ezinokhokho oyedwa.
    I-PCA ihlose ukubona ngeso lengqondo ukusondelana phakathi kwabantu abancane.
    Ukwakheka kwenani labantu kubonisa ukuba khona kwenani labantu abancane abahlukene ngokofuzo ngokuya ngamaza e-allele.

    3-1Isihlahla se-Phylogenetic 3-2PCA 3-3Isakhiwo-sabantu

    Chen, njll.al.,I-PNAS, 2020

    2.Ukushanela okukhethiwe

    Ukushanela okukhethiwe kusho inqubo lapho kukhethwa khona isayithi elinenzuzo futhi amaza amasayithi axhumene angathathi hlangothi ayanda futhi lawo amasayithi angaxhunywanga ancipha, okuholela ekuncishisweni kwesifunda.

    Ukutholwa okubanzi kwe-genome ezindaweni ezishanelayo ezikhethiwe kucubungula ngokubala inkomba yofuzo labantu (π,Fst, Tajima's D) yawo wonke ama-SNP ngaphakathi kwewindi elislayidayo (100 Kb) esinyathelweni esithile (10 KB).

    Ukuhlukahluka kwe-Nucleotide (π)
    I-4Nucleotide-diversity(π)

    UTajima D
    5Tajima's-D

    Inkomba yokulungisa(Fst)

    I-6Fixation-index(Fst)

    Wu, et.al.,Isitshalo samangqamuzana, 2018

    3.Ukugeleza kweGene

    7Ukugeleza kofuzo

    Wu, et.al.,Isitshalo samangqamuzana, 2018

    4.Umlando wezibalo zabantu

    8Umlando wabantu

    Zhang, njll.al.,I-Nature Ecology & Evolution, 2021

    5.Isikhathi sokuhlukana

    9Isikhathi sokuhlukana

    Zhang, njll.al.,I-Nature Ecology & Evolution, 2021

    Icala le-BMK

    Imephu yokuhlukahluka kofuzo inikeza imininingwane ngesisekelo sofuzo se-Spring Chinese Cabbage(Brassica rapa ssp. Pekinensis)

    Kushicilelwe: Isitshalo samangqamuzana, 2018

    Isu lokulandelanisa:

    Ukulandelanisa: ukujula kokulandelana: 10×

    Imiphumela esemqoka

    Kulolu cwaningo, amaklabishi aseShayina angu-194 acutshungulwa ukuze aphinde alandelane ngokujula okuphakathi kuka-10×, okukhiqize ama-SNP angu-1,208,499 kanye nama-InDels angu-416,070.Ukuhlaziywa kwe-Phylogenetic kule migqa engu-194 kuboniswe ukuthi le migqa ingahlukaniswa ngezinhlobo ezintathu ze-ecotypes, intwasahlobo, ihlobo kanye nekwindla.Ukwengeza, ukwakheka kwenani labantu kanye nokuhlaziywa kwe-PCA kubonise ukuthi iklabishi laseShayina lasentwasahlobo lavela ekwindla yeklabishi eShandong, eChina.Lezi zabe zethulwa e-Korea nase-Japan, zaphambana nemigqa yasendaweni futhi ezinye izinhlobo zazo ezifakwa sekwephuzile zalethwa emuva e-China futhi ekugcineni zaba iklabishi lase-Spring Chinese.

    Ukuskena okubanzi kwe-genome kumaklabishi asentwasahlobo aseShayina kanye neklabishi yasekwindla ekukhethweni kwembula izindawo ezingama-23 ze-genomic eziye zakhethwa ngokuqinile, ezimbili zazo zigqitshwe ngesifunda sokulawula isikhathi sokubhowula okusekelwe kumephu ye-QTL.Lezi zifunda ezimbili zitholwe zinezakhi zofuzo ezibalulekile ezilawula ukuqhakaza, i-BrVIN3.1 kanye ne-BrFLC1.Lezi zakhi zofuzo ezimbili zaqinisekiswa futhi ukuthi ziyabandakanyeka ekuhlanganiseni isikhathi ngocwaningo lwe-transcriptome kanye nokuhlolwa kwe-transgenic.

    I-PB-full-length-RNA-Sequencing-case-study

    Ukuhlaziywa kwesakhiwo sabantu kumaklabishi aseShayina

    I-PB-full-length-RNA-alternative-splicing

    Ulwazi lwezofuzo ekukhethweni kweklabishi laseShayina

     
    Ireferensi

    Tongbing, et al."Imephu Yokuhlukahluka Kwe-Genomic Ihlinzeka Ngemininingwane Ngesisekelo Sofuzo Se-Spring Chinese Cabbage (Brassica rapa ssp.pekinensis)Selection."Izitshalo zamangqamuzana,11(2018):1360-1376.

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