Takagi et al.,Ijenali yezitshalo, 2013
● Ukulinganisa isikhathi sokuhlukana kwezinhlobo zezilwane nesivinini ngokusekelwe ezinhlobonhlobo zeleveli yama-nucleotide nama-amino acid.
● Ukwembulwa kobudlelwane obuthembeke kakhulu be-phylogenetic phakathi kwezinhlobo zezilwane ezinethonya elincishisiwe lokuguquguquka okuguquguqukayo kanye nokuziphendukela kwemvelo okufanayo
● Ukwakha izixhumanisi phakathi kwezinguquko zofuzo nama-phenotypes ukuze kwambulwe izakhi zofuzo ezihlobene nezici
● Ukulinganisa ukuhlukahluka kwezakhi zofuzo, okubonisa amandla okuziphendukela kwemvelo kwezinhlobo zezinto eziphilayo
● Isikhathi sokushintsha esisheshayo
● Okuhlangenwe nakho okubanzi: I-BMK iqongelele ulwazi olukhulu ngenani labantu kanye namaphrojekthi ahlobene nokuziphendukela kwemvelo iminyaka engaphezu kwengu-12, ehlanganisa amakhulu ezinhlobo zezilwane, njll. futhi yanikela kumaphrojekthi wezinga eliphezulu angaphezu kuka-80 ashicilelwe ku-Nature Communications, Izitshalo Zemolekyuli, I-Plant Biotechnology Journal, njll.
Izinto zokwakha:
Ngokujwayelekile, kunconywa okungenani izigaba ezintathu ezincane (isib. izinhlobonhlobo noma izinhlobo).Inani elincane ngalinye kufanele libe nabantu abangekho ngaphansi kuka-10 (Izitshalo >15, zingancishiselwa izinhlobo ezingavamile).
Isu lokulandelanisa:
* I-WGS ingaqashwa ezinhlotsheni ezine-genome yereferensi yekhwalithi ephezulu, kuyilapho i-SLAF-Seq isebenza ezinhlotsheni ezine-genome eyisithenjwa noma ezingenayo, noma i-genome eyireferensi yekhwalithi embi.
Isebenza kusayizi wofuzo | WGS | Amathegi e-SLAF (×10,000) |
≤ 500 Mb | 10×/ngamunye | I-WGS inconywa kakhulu |
500 Mb - 1 Gb | 10 | |
1 Gb - 2 Gb | 20 | |
≥2 Gb | 30 |
● Ukuhlaziya kokuziphendukela kwemvelo
● Ukushanela okukhethekile
● Ukugeleza kofuzo
● Umlando wezibalo zabantu
● Isikhathi sokuhlukana
Izinhlobo | Izicubu | I-WGS-NGS | I-SLAF |
Isilwane
| Izicubu ze-Visceral |
0.5~1g
|
0.5g
|
Izicubu zemisipha | |||
Igazi lezilwane ezincelisayo | 1.5mL
| 1.5mL
| |
Igazi lezinkukhu/Inhlanzi | |||
Isitshalo
| Iqabunga Elisha | 1~2g | 0.5~1g |
I-Petal/Isiqu | |||
Impande/Imbewu | |||
Amaseli | Iseli Cultured |
gDNA | Ukugxila | Inani (ug) | OD260/OD280 |
I-SLAF | ≥35 | ≥1.6 | 1.6-2.5 |
I-WGS-NGS | ≥1 | ≥0.1 | - |
*Imiphumela yedemo eboniswe lapha yonke ivela kuzakhi zofuzo ezishicilelwe nge-BMKGENE
Ukuhlaziywa kwe-1.Evolution kuqukethe ukwakhiwa kwesihlahla se-phylogenetic, isakhiwo sabantu kanye ne-PCA ngokusekelwe ezinhlobonhlobo zofuzo.
Isihlahla se-Phylogenetic simele ubudlelwano be-taxonomic kanye nokuziphendukela kwemvelo phakathi kwezinhlobo ezinokhokho oyedwa.
I-PCA ihlose ukubona ngeso lengqondo ukusondelana phakathi kwabantu abancane.
Ukwakheka kwenani labantu kubonisa ukuba khona kwenani labantu abancane abahlukene ngokofuzo ngokuya ngamaza e-allele.
Chen, njll.al.,I-PNAS, 2020
2.Ukushanela okukhethiwe
Ukushanela okukhethiwe kusho inqubo lapho kukhethwa khona isayithi elinenzuzo futhi amaza amasayithi axhumene angathathi hlangothi ayanda futhi lawo amasayithi angaxhunywanga ancipha, okuholela ekuncishisweni kwesifunda.
Ukutholwa okubanzi kwe-genome ezindaweni ezishanelayo ezikhethiwe kucubungula ngokubala inkomba yofuzo labantu (π,Fst, Tajima's D) yawo wonke ama-SNP ngaphakathi kwewindi elislayidayo (100 Kb) esinyathelweni esithile (10 KB).
Ukuhlukahluka kwe-Nucleotide (π)
UTajima D
Inkomba yokulungisa(Fst)
Wu, et.al.,Isitshalo samangqamuzana, 2018
3.Ukugeleza kweGene
Wu, et.al.,Isitshalo samangqamuzana, 2018
4.Umlando wezibalo zabantu
Zhang, njll.al.,I-Nature Ecology & Evolution, 2021
5.Isikhathi sokuhlukana
Zhang, njll.al.,I-Nature Ecology & Evolution, 2021
Icala le-BMK
Imephu yokuhlukahluka kofuzo inikeza imininingwane ngesisekelo sofuzo se-Spring Chinese Cabbage(Brassica rapa ssp. Pekinensis)
Kushicilelwe: Isitshalo samangqamuzana, 2018
Isu lokulandelanisa:
Ukulandelanisa: ukujula kokulandelana: 10×
Imiphumela esemqoka
Kulolu cwaningo, amaklabishi aseShayina angu-194 acutshungulwa ukuze aphinde alandelane ngokujula okuphakathi kuka-10×, okukhiqize ama-SNP angu-1,208,499 kanye nama-InDels angu-416,070.Ukuhlaziywa kwe-Phylogenetic kule migqa engu-194 kuboniswe ukuthi le migqa ingahlukaniswa ngezinhlobo ezintathu ze-ecotypes, intwasahlobo, ihlobo kanye nekwindla.Ukwengeza, ukwakheka kwenani labantu kanye nokuhlaziywa kwe-PCA kubonise ukuthi iklabishi laseShayina lasentwasahlobo lavela ekwindla yeklabishi eShandong, eChina.Lezi zabe zethulwa e-Korea nase-Japan, zaphambana nemigqa yasendaweni futhi ezinye izinhlobo zazo ezifakwa sekwephuzile zalethwa emuva e-China futhi ekugcineni zaba iklabishi lase-Spring Chinese.
Ukuskena okubanzi kwe-genome kumaklabishi asentwasahlobo aseShayina kanye neklabishi yasekwindla ekukhethweni kwembula izindawo ezingama-23 ze-genomic eziye zakhethwa ngokuqinile, ezimbili zazo zigqitshwe ngesifunda sokulawula isikhathi sokubhowula okusekelwe kumephu ye-QTL.Lezi zifunda ezimbili zitholwe zinezakhi zofuzo ezibalulekile ezilawula ukuqhakaza, i-BrVIN3.1 kanye ne-BrFLC1.Lezi zakhi zofuzo ezimbili zaqinisekiswa futhi ukuthi ziyabandakanyeka ekuhlanganiseni isikhathi ngocwaningo lwe-transcriptome kanye nokuhlolwa kwe-transgenic.
Ukuhlaziywa kwesakhiwo sabantu kumaklabishi aseShayina | Ulwazi lwezofuzo ekukhethweni kweklabishi laseShayina |
Tongbing, et al."Imephu Yokuhlukahluka Kwe-Genomic Ihlinzeka Ngemininingwane Ngesisekelo Sofuzo Se-Spring Chinese Cabbage (Brassica rapa ssp.pekinensis)Selection."Izitshalo zamangqamuzana,11(2018):1360-1376.