I-Genomics yokuqhathanisa
Izinzuzo Zesevisi
● Iphakheji yokuhlaziya okubanzi, equkethe ukuhlaziya okuyisishiyagalombili okudingekile kakhulu
● Ukuthembeka okuphezulu ekuhlaziyeni okunencazelo enemininingwane neqondakala kalula ngemiphumela
● Izibalo eziklanywe kahle ezilungele ukushicilelwa
● Ithimba le-bioinformatics elinamakhono aphezulu lifeza izimfuno ezihlukene zokuhlaziya komuntu siqu
● Isikhathi esifushane sokuguqula ngokunemba okuphezulu ekuhlaziyeni
● Okuhlangenwe nakho okuchichimayo okunezimo eziphumelele ezingaphezu kuka-90 ezinethonya elihlanganisiwe elishicilelwe elingaphezu kuka-900
Imininingwane Yesevisi
| Isikhathi sokujika esilinganiselwe | Inombolo yezinhlobo | Iyahlaziya |
| 30 izinsuku zokusebenza | 6-12 | Ukuhlanganiswa komndeni weGene Ukwandiswa komndeni weGene kanye nokufinyela Ukwakhiwa kwesihlahla se-Phylogenetic Ukulinganisa isikhathi sokuhlukana (kudingeka ukulinganisa izinsalela zamathambo) Isikhathi sokufakwa kwe-LTR (Okwezitshalo) Ukuphindaphinda kofuzo lonke (Okwezitshalo) Ingcindezi ekhethiwe Ukuhlaziywa kwe-Synteny |
I-Bioinformatics ihlaziya
● Umndeni wakwaGene
● I-Phylogenetics
● Isikhathi sokuhlukana
● Ukucindezela okukhethekile
● Ukuhlaziya i-synteny
Izidingo Zesampula Nokulethwa
Izimfuneko zesampula:
Izicubu noma i-DNA yokulandelana kwe-genome nokuhlanganisa
Okwezicubu
| Izinhlobo | Izicubu | Inhlolovo | I-PacBio CCS |
| Isilwane | Izicubu ze-Visceral | 0.5 ~ 1g | ≥ 3.5g |
| Izicubu zemisipha | |||
| ≥ 5.0g | |||
| ≥ 5.0mL | |||
| Igazi lezilwane ezincelisayo | |||
| ≥ 0.5mL | |||
| Igazi lezinkukhu/Inhlanzi | |||
| Isitshalo | Iqabunga Elisha | 1 ~ 2g | ≥ 5.0g |
| I-Petal/Isiqu | 1 ~ 2g | ≥ 10.0g | |
| Impande/Imbewu | 1 ~ 2g | ≥ 20.0g | |
| Amaseli | Iseli Cultured | - | ≥ 1 x 108 |
Idatha
Amafayela okulandelana kwe-genome(.fasta) namafayela ezichasiselo (.gff3) ezinhlobo ezihlobene eduze
Ukugeleza Komsebenzi Wesevisi
Idizayini yokuhlola
Ukulethwa kwesampula
Ukwakhiwa komtapo wolwazi
Ukulandelanisa
Ukuhlaziywa kwedatha
Amasevisi angemuva kokuthengisa
*Imiphumela yedemo eboniswe lapha yonke ivela kuzakhi zofuzo ezishicilelwe nge-Biomarker Technologies
1.Isilinganiso sesikhathi sokufaka kwe-LTR: Isibalo esiboniswe ukusabalalisa okuhlukile kwe-bimodal ezikhathini zokufakwa ze-LTR-RTs ku-Weining rye genome, uma kuqhathaniswa nezinye izinhlobo.Inani eliphakeme kakhulu lakamuva lavela cishe eminyakeni eyizigidi ezingu-0.5 edlule.
U-Li Guang et al.,I-Nature Genetics, 2021
2.I-Phylogeny ne-gene family analysis on chayote (Sechium edule) : Ngokuhlaziya i-chayote nezinye izinhlobo ze-13 ezihlobene emndenini wezakhi zofuzo, i-Chayote itholakale ihlobene kakhulu ne-snake gourd (Trichosanthes anguina).I-Chayote ethathwe kumhlava wenyoka cishe ku-27-45 Mya kanye nokuphindaphindwa kwegenome ephelele(WGD) yabonwa ku-chayote ku-25±4 Mya, okuwumcimbi wesithathu we-WGD ku-cucuibitaceae.
Fu A et al.,Ucwaningo Lwezolimo, 2021
3.Ukuhlaziywa kwe-Synteny: Ezinye izakhi zofuzo ezihlobene nama-phytohormones ekuthuthukisweni kwezithelo zatholakala ku-chayote, i-snake gourd kanye ne-squash.Ukuhlobana phakathi kwe-chayote ne-squash kuphakeme kancane kunalokho okuphakathi kwe-chayote ne-snake gourd.
Fu A et al.,Ucwaningo Lwezolimo, 2021
Ukuhlaziywa komndeni kwe-4.Gene: Ukuthuthukiswa kwe-KEGG ekwandeni komndeni wofuzo kanye nokufinyela ku-G.thurberi kanye ne-G.davidsonii genomes kuboniswe ukuthi i-steroid biosynthesis kanye nezakhi zofuzo ezihlobene ne-brassinosteroid biosynthesis zandisiwe.
Yang Z et al.,I-BMC Biology, 2021
5.Ukuhlaziywa kokuphindwaphindwa kwegenome okuphelele: Ukuhlaziywa kokusabalalisa kwe-4DTV kanye ne-Ks kuboniswa wonke umcimbi wokuphindaphinda igenome.Iziqongo ze-intraspecies ezibonisiwe izehlakalo eziyimpinda.Iziqongo ze-interspecies ezibonisiwe izehlakalo ze-speciation.Ukuhlaziya kubonise ukuthi uma kuqhathaniswa nezinye izinhlobo ezintathu ezisondelene kakhulu, i-O. europaea idlule ekuphindaphindweni kwezakhi zofuzo ngezinga elikhulu muva nje.
URao G et al.,Ucwaningo Lwezolimo, 2021
Icala le-BMK
I-Rose ngaphandle kwe-prickle: imininingwane ye-genomic ehlotshaniswa nokujwayela umswakama
Kushicilelwe: Ukubuyekezwa Kwesayensi Kazwelonke, 2021
Isu lokulandelanisa:
'BasyeEngenameva' (R.WichurainanI-genome:
Cishe.93 X PacBio + cishe.90 X Nanopore + 267 X Illumina
Imiphumela esemqoka
1.Ikhwalithi ephezulu ye-R.wichuraiana genome yakhiwe kusetshenziswa amasu okulandelana okufundwe isikhathi eside, akhiqiza ukuhlanganisa okungu-530.07 Mb (Usayizi wegenome olinganiselwe wawucishe ube ngu-525.9 Mb nge-flow cytometry kanye no-525.5 nge-genome survey; I-Heterozygosity yayicishe ibe ngu-1.03%).I-BUSCO ilinganiselwa ku-93.9%.Uma kuqhathaniswa ne-“Old blush” (haploOB), ikhwalithi nokuphelela kwale genome kwaqinisekiswa ukunemba kwesisekelo esisodwa kanye nenkomba yomhlangano we-LTR (LAI=20.03).I-R.wichuraiana genome iqukethe ama-protein coding genome angu-32,674.
2.Ukuhlaziywa okuhlanganyelwe kwe-Multi-omics, okuhlanganisa ukuqhathanisa i-genomics, i-transcriptomics, ukuhlaziywa kwe-QTL yezakhi zofuzo, kwembula ukucaciswa okubalulekile phakathi kuka-R. wichuraiana ne-Rosa chinensis.Futhi, ukuhluka kwenkulumo yezakhi zofuzo ezihlobene ku-QTL kungenzeka zihlotshaniswe nephethini ye-stem prickle.
Ukuhlaziywa kwe-genomics yokuqhathanisa phakathi kwe-Basye;s Thornless ne-Rosa chinensis okuhlanganisa ukuhlaziywa kwe-synteny, iqoqo lomndeni wofuzo, ukuhlaziywa kokunwetshwa nokufinyela, kwembula inani elikhulu lokuhlukahluka, elihlobene nezici ezibalulekile zama roses.Ukwanda okuyingqayizivele ku-NAC kanye nomndeni we-FAR1/FRS wofuzo kungenzeka kakhulu ukuthi uhlotshaniswe nokumelana nendawo emnyama.
Ukuhlaziywa kwe-genomics yokuqhathanisa phakathi kwe-BT ne-haploOB genomes.
Zhong, M., et al."I-Rose ngaphandle kwe-prickle: imininingwane ye-genomic exhunywe nokujwayela umswakama"Ukubuyekezwa Kwesayensi Kazwelonke, 2021;, wab092.
