Imikhiqizo

Amagciwane Aphelele I-Genome

I-bacterium Qedela i-Genome Isithombe Esifakiwe

I-Biomarker Technologies ihlinzeka ngensizakalo yokulandelanisa ekwakheni i-genome ephelele yebhaktheriya enegebe eliziro.Ukuhamba komsebenzi okuyinhloko kwebhaktheriya ukwakhiwa okuphelele kofuzo kufaka phakathi ukulandelana kwesizukulwane sesithathu, ukuhlanganisa, isichasiselo esisebenzayo kanye nokuhlaziywa okuthuthukisiwe kwe-bioinformatic okufeza imigomo ethile yocwaningo.Iphrofayili ebanzi yofuzo lwebhaktheriya inika amandla ukudalulwa kwezinqubo ezibalulekile eziyisisekelo zezinqubo zabo zebhayoloji, okungase futhi kunikeze ireferensi ebalulekile yocwaningo lwe-genomic ezinhlotsheni eziphakeme ze-eukaryotic.

Inkundla:I-Nanopore PromethION P48 + Illumina NovaSeq Platform

I-PacBio Sequel II

Xhumana nathi

Imininingwane Yesevisi

Imiphumela yedemo

Izinzuzo Zesevisi

● Amasu amaningi okulandelana atholakalayo ezinhlosweni ezihlukene zocwaningo

● I-genome egcwele amagciwane ene-Gap engu-0 eqinisekisiwe.

● Unolwazi oluphezulu ekuhlanganiseni kofuzo lwebhaktheriya ehlanganisa i-genome engaphezu kuka-10,000.

● Ithimba losekelo lobuchwepheshe ngemva kokuthengisa elenza izidingo ezithile zocwaningo.

Imininingwane Yesevisi

UkulandelanisaIsu

Umtapowolwazi

Ikhwalithi iqinisekisiwe

Isikhathi sokujika esilinganiselwe

I-Nanopore 100X + Illumina 50X

I-Nanopore 20K

I-PE150

0 igebe

Izinsuku ezingama-30

I-PacBio HiFi 30X

I-PacBio 10K

I-Bioinformatics ihlaziya

● Ukulawulwa kwekhwalithi yedatha eluhlaza

● Ukuhlanganiswa kwe-genome

● Ukuhlaziywa kwengxenye ye-genome

● Isichasiselo somsebenzi wofuzo

● Ukuhlaziywa kwe-genomic okuqhathanisayo

Izidingo Zesampula Nokulethwa

Izimfuneko zesampula:

NgobaIzingcaphuno ze-DNA:

Uhlobo lwesampula	Inani	Ukugxila	Ubumsulwa
Izingcaphuno ze-DNA	＞ 2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Okwamasampula ezicubu:

Uhlobo lwesampula

Ukwelashwa okuyisampula okunconyiwe

Isampula yokugcina nokuthunyelwa

Amagciwane

Bheka amagciwane ngaphansi kwesibonakhulu futhi uqoqe amagciwane esigabeni sawo sokuchayeka

Dlulisa isiko lebhaktheriya (eliqukethe cishe amaseli angu-3-4.5e9) ku-1.5 noma 2 ml eppendorf.(Qhubekela eqhweni)

I-Centrifuge ishubhu iminithi elingu-1 ku-14000 g ukuqoqa amabhaktheriya futhi ususe i-supernatant ngokucophelela.

Vala ishubhu bese ufriza amabhaktheriya ku-nitrogen ewuketshezi okungenani imizuzu engama-30.Gcina ishubhu ku -80 ℃ esiqandisini.

Friza amasampula ku-nitrogen ewuketshezi amahora angu-3-4 futhi uwagcine ku-nitrogen ewuketshezi noma -80 degree ekubhukeni isikhathi eside.Kudingeka isampula yokuthunyelwa ngeqhwa elomile.