Idinku Aṣoju Bisulfite Sequencing (RRBS)
Awọn pato Iṣẹ
Platform: NovaSeq Syeed, PE150
Iru ikawe: 200-400bp fi sii bisulfite ti a ṣe itọju ile-ikawe DNA
Ilana ilana: Paired-Opin 150 bp
Ṣe iṣeduro igbejade data: 10 Gb aise data/apẹẹrẹ
Awọn ibeere apẹẹrẹ
Iwọn DNA: ≥ 2ug
Ifojusi DNA: ≥ 20ng/μl.
Mimọ: OD260/280 = 1.8 si 2.0 laisi ibajẹ tabi ibajẹ RNA
Itupalẹ Bioinformatics
Sisan Iṣẹ Iṣẹ
Ifijiṣẹ apẹẹrẹ
Ikole ìkàwé
Titele
Itupalẹ data
Lẹhin-tita awọn iṣẹ
a.Awọn iṣiro titete jiini itọkasi
Awọn iṣiro ti awọn kika maapu
Sawọn iṣiro ti ijinle lẹsẹsẹ ati agbegbe ti aaye C
Aakojo agbegbe
Digestion ṣiṣe ti kọọkan ayẹwo
b.Wiwa aaye Methylation
Akojọ ti awọn ipele methylation ni aaye C
BS iyipada
Mcytosine ethylated
Methylation ipele pinpin
c.Methylation maapu
Genome-jakejado
Genome eroja
d.Ifiwera awọn ipele methylation laarin awọn ayẹwo
Pairwise Ibamu ti CG methylation
PCA-CG methylation
Clustering ti CG methylation
d.Iyatọ methylation onínọmbà
Sawọn iṣiro ti DMR
DMR ipari pinpin
Hjẹ maapu
DMR pinpin ni agbegbe ìfọkànsí
