Ilana RNA Prokaryotic

Ilana RNA Prokaryotic nlo ilana iran atẹle (NGS) lati ṣafihan wiwa ati opoiye ti RNA ni akoko ti a fun, nipa ṣiṣe itupalẹ iyipada transcriptome cellular.Ilana ilana prokaryotic RNA ti ile-iṣẹ wa, ni pataki ni ifọkansi si awọn prokaryotes pẹlu awọn genomes itọkasi, pese fun ọ pẹlu profaili transscriptome, itupalẹ igbekalẹ pupọ, ati bẹbẹ lọ O ti lo jakejado si iwadii imọ-jinlẹ ipilẹ, iwadii oogun ati idagbasoke, ati diẹ sii.

Platform: Illumina NovaSeq Platform

Pe wa

Awọn alaye Iṣẹ

Awọn pato Iṣẹ

Platform: Illumina NovaSeq Syeed, PE150

Iru ile ikawe: 350bp fi sii ile-ikawe cDNA (da lori pinpin tente oke)

Ilana ilana: Paired-Opin 150 bp

Ṣe iṣeduro iye data: 2 Gb aise data/apẹẹrẹ

Bioinformatics

(1) Ṣiṣeto iṣakoso didara data: pinpin Nucleotide, pinpin didara ipilẹ, iṣiro ipin rRNA;

(2) Atupalẹ titete lẹsẹsẹ: Awọn iṣiro abajade isọdọtun, Saturation of sequencing, Ibora ti ilana;

(3) Itọkasi genome annotation (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Itupalẹ ikosile: pinpin ikosile (Pẹlu awọn ayẹwo meji tabi diẹ ẹ sii), Itupalẹ ti ikosile laarin awọn ayẹwo (Itupalẹ Venn, Itupalẹ Atunse, PCA onínọmbà);

(5) Ayẹwo ikosile iyatọ (Pẹlu awọn ayẹwo meji tabi diẹ sii);

(6) Iṣiro ipilẹ Gene: Ayẹwo Venn, Itupalẹ iṣupọ, Itupalẹ akọsilẹ iṣẹ (COG, GO, KEGG), Iṣiro imudara iṣẹ-ṣiṣe (GO, KEGG), Iwọn imudara iṣẹ-ṣiṣe chordal, Ipath onínọmbà;

(7) itupalẹ sRNA: asọtẹlẹ sRNA, asọye sRNA (Blast, sRNAMap, sRNATarBase, SIPHT, Rfam), asọtẹlẹ igbekalẹ elekeji sRNA, asọtẹlẹ jiini afojusun sRNA;

(8) Itupalẹ igbekalẹ tiransikiripiti: Itupalẹ Operon, Ibẹrẹ ikọsilẹ ati asọtẹlẹ awọn aaye ipari, asọye UTR ati itupalẹ, Asọtẹlẹ ọkọọkan Olugbega, SNP/InDel onínọmbà (SNP/InDel annotation, SNP/InDel pinpin agbegbe, awọn iṣiro SNP).