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GENOME IDAGBASOKE

Genome De novo
Apejọ|Ipinnu ibalopo Gbogbo awọn iṣẹ ṣiṣe lẹsẹsẹ ati awọn iṣẹ bioinformatic apakan ni a pese nipasẹ Biomarker Techonologies.

Áljẹbrà

"Ẹya aṣebiakọ ti seadragons pẹlu awọn ohun elo ti o dabi ewe, ẹnu tubular ti ko ni ehin, ati oyun akọ ti o kan isunmọ ti awọn ẹyin ajinde lori “patch brood” ṣiṣi.We de novo-ti o tẹle akọ ati abo genomes ti seadragon ti o wọpọ (Phyllopteryx taeniolatus) ati awọn eya ti o ni ibatan pẹkipẹki, alligator pipefish (Syngnathoides biaculeatus).Awọn profaili transcription lati aratuntun ti itiranya, awọn ohun elo ti o dabi ewe, fihan pe akojọpọ awọn jiini ti o ni ipa ninu idagbasoke fin ni a ti ṣajọpọ daradara bi imudara awọn iwe afọwọkọ tran fun atunṣe àsopọ ti o pọju ati awọn jiini aabo aabo.Awọn mutanti zebrafish fun scpp5, eyiti o sọnu ni gbogbo syngnathids, ni a rii pe ko ni tabi ni awọn ehin pharyngeal ti o bajẹ, ti o ṣe atilẹyin idawọle pe isonu ti scpp5 ti ṣe alabapin si isonu ti eyin ni syngnathids.Ibalopo putative – npinnu agbegbe ti n ṣe koodu ti jiini amhr2y kan pato akọ ti o pin nipasẹ seadragon ti o wọpọ ati alligator pipefish jẹ idanimọ. 

Awon nkan ise nkan ati awon ona lati se nkan:

Awọn ohun elo

FSeadragon ti o wọpọ wa (P. taeniolatusati pipefish meji alligator (S. biaculeatus) awọn apẹẹrẹ.Seadragon meji ti o wọpọ (ọkunrin kan ati obinrin kan) ati pipefish alligator meji (ọkunrin kan ati obinrin kan) awọn ẹni-kọọkan ni a lo fun ilana-ara-ara-ara, ati awọn ọkunrin meji miiran ti o wọpọ seadragon kọọkan ni a lo fun atunṣe genome.

Jiini Sequencing nwon.Mirza

270/350 bp ìkàwé (Hiseq 2500) + Pacbio 20Kb (Sequel) tabi Nanopore 30 Kb (MinION)+ Hi-C (NovaSeq 6000) .sequencing, ati awọn ọkunrin meji miiran ti o wọpọ seadragon kọọkan ni a lo fun atunṣe genome.

Transcriptome Sequencing

Ftabi seadragon ti o wọpọ, ọpọlọ, oju, gill, ẹdọ, ọkan, kidinrin, mesentery, ifun, iṣan, fin, awọ ara, awọn ohun elo ti o dabi ewe, testis, ati ovary ni a gba.

Genome de novo ijọ nwon.Mirza

Canu (Atunse) + WTDBG2 (Apejọ) + Pilon (Polish) + Lachesis (Hi-C).

Awọn abajade akọkọ

Aworan-1.-Awọn ẹya ara ẹrọ bọtini-ti-ti-wọpọ-seadragon-P.-taeniolatus-ati-alligator-pipefish-S

Aworan 1. Awọn ẹya pataki ti seadragon ti o wọpọ (P. taeniolatus) ati pipefish alligator (S. biaculeatus) ati awọn ipo phylogenetic wọn.

Aworan.-2.-Morphology-ati-jiini-atike-ti-leaf-bi-appendages-in-the-wọpọ-seadragon-P

aworan.

Aworan-3.-Putative-ibalopo-ipinnu-gene-ni-wọpọ-seadragon-P.-taeniolatus-ati-alligator-pipefish-S.-biaculeatus

Aworan 3. Jiini ipinnu ibalopo Putative ni seadragon ti o wọpọ (P. taeniolatus) ati pipefish alligator (S. biaculeatus).

olusin-4

aworan 4. Pharyngeal ehin phenotypes ni zebrafish scpp5 homozygous mutanti.


Akoko ifiweranṣẹ: Jan-08-2022

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