GENOME IDAGBASOKE, PANGENOME
Kini Pan-genome?
Awọn ẹri ti a kojọpọ fihan pe iyatọ laarin awọn oriṣiriṣi oriṣiriṣi ti eya le jẹ nla.Ẹyọ apilẹ̀ àbùdá ẹyọ kan jìnnà tó láti jèrè gbogbo àwòrán ìsọfúnni àbùdá ẹ̀yà kan ṣoṣo.Idi ti iwadi Pan-genome ni lati gba aworan jiini ti o ni kikun diẹ sii ti eya kan ati pinnu awọn ibatan laarin awọn abuda ati awọn koodu jiini nipasẹ ṣiṣe apejọ genome de novo ti ọpọlọpọ awọn igara, eyiti o fun laaye iwakusa jinlẹ ati gbooro ti awọn iyatọ.
Awọn aṣa ti Pan-genome Ìkẹkọọ
Ṣe nọmba 1 Awọn aṣa ti awọn iwe iwadi ti a tẹjade ti Pan-genome.
Akiyesi: Nọmba naa fihan abajade ti gbigba “pan-genome” gẹgẹbi ọrọ pataki lati wa awọn akọle ti awọn nkan ti a tẹjade ni Awọn iwe iroyin Iseda, Ẹjẹ ati Imọ-jinlẹ
a.Awọn kika lati awọn ayẹwo lọpọlọpọ ti wa ni ibamu si itọkasi kan ati pe awọn ti ko ni ibamu ni a pejọ sinu awọn contigs aramada.Nipa fifi awọn contigs aramada wọnyi kun si ọna itọka atilẹba, itọkasi pangenome le jẹ itumọ.Awọn agbegbe dispensable jẹ ipinnu da lori aworan agbaye gbogbo awọn kika pada si pangenome.
b.Apejọ De novo ti awọn genomes ti awọn ẹya ara ẹrọ lọpọlọpọ ngbanilaaye gbogbo awọn isunmọ titete genome lati ṣe idanimọ awọn agbegbe genomic dispensable.
c.Aworan pan-genome le ṣe agbekalẹ lati gbogbo awọn tito genome tabi nipasẹ apejọ aworan de novo, eyiti o tọju daradara alaye iyatọ ti awọn agbegbe dispensable bi awọn ọna alailẹgbẹ nipasẹ iwọn.
Bawo ni lati kọ Pan-genome kan?
Aworan 2 Ifiwera awọn isunmọ pan-genome1
a.Awọn kika lati awọn ayẹwo lọpọlọpọ ti wa ni ibamu si itọkasi kan ati pe awọn ti ko ni ibamu ni a pejọ sinu awọn contigs aramada.Nipa fifi awọn contigs aramada wọnyi kun si ọna itọka atilẹba, itọkasi pangenome le jẹ itumọ.Awọn agbegbe dispensable jẹ ipinnu da lori aworan agbaye gbogbo awọn kika pada si pangenome.
b.Apejọ De novo ti awọn genomes ti awọn ẹya ara ẹrọ lọpọlọpọ ngbanilaaye gbogbo awọn isunmọ titete genome lati ṣe idanimọ awọn agbegbe genomic dispensable.
c.Aworan pan-genome le ṣe agbekalẹ lati gbogbo awọn tito genome tabi nipasẹ apejọ aworan de novo, eyiti o tọju daradara alaye iyatọ ti awọn agbegbe dispensable bi awọn ọna alailẹgbẹ nipasẹ iwọn.
Laipe Atejade Pan-genomes
● ifipabanilopo pan-genomes2
● Awọn tomati pan-genomes 3
● Rice Pan-genome4
● Sunflower Pan-genome5
● Soybean Pan-genome 6
● Rice Pan-genome7
● Barle Pan-genome8
● Alikama Pan-genome9
● Sorghum Pan-genome10
● Phytoplankton Pan-genome11
Itọkasi
1. Bayer PE, Golicz AA, Scheben A, Batley J, Edwards D. Plant pan-genomes jẹ itọkasi tuntun.Awọn ohun ọgbin Nat.2020; 6 (8): 914-920.doi:10.1038/s41477-020-0733-0
2. Orin JM, Guan Z, Hu J, et al.Awọn genomes didara giga mẹjọ ṣafihan faaji pan-genome ati iyatọ ecotype ti Brassica napus.Awọn ohun ọgbin Nat.2020; 6 (1): 34-45.doi:10.1038/s41477-019-0577-7
3. Gao L, Gonda I, Sun H, et al.Awọn tomati pan-genome ṣe awari awọn jiini tuntun ati allele ti o ṣọwọn ti n ṣakoso adun eso.Nat Genet.2019;51 (6): 1044-1051.doi:10.1038/s41588-019-0410-2
4. Zhao Q, Feng Q, Lu H, et al.Itupalẹ Pan-genome ṣe afihan iwọn iyatọ jiini ninu awọn gbin ati iresi igbẹ [atunse ti a tẹjade han ni Nat Genet.Ọdun 2018 Oṣu Kẹjọ; 50 (8): 1196].Nat Genet.2018;50 (2):278-284.doi:10.1038/s41588-018-0041-z
5. Hübner S, Bercovich N, Todesco M, et al.Itupalẹ pan-genome sunflower fihan pe isọdọkan yipada akoonu jiini ati resistance arun.Awọn ohun ọgbin Nat.Ọdun 2019; 5 (1): 54-62.doi:10.1038/s41477-018-0329-0
6. Liu Y, Du H, Li P, et al.Pan-Genome of Wild ati gbin Soybean.Ẹyin sẹẹli.2020;182(1):162-176.e13.doi:10.1016/j.cell.2020.05.023
7. Qin P, Lu H, Du H, et al.Atupalẹ Pan-genome ti 33 oniruuru oniruuru iresi awọn ẹya ara ẹrọ jiini ṣe afihan awọn iyatọ jiini ti o farapamọ [ti a tẹjade lori ayelujara ṣaaju titẹ, 2021 May 25].Ẹyin sẹẹli.2021; S0092-8674 (21) 00581-X.doi: 10.1016 / j.cell.2021.04.046
8. Jayakodi M, Padmarasu S, Haberer G, et al.Awọn pan-genome barle ṣe afihan ohun-ini ti o farapamọ ti ibisi iyipada.Iseda.2020;588(7837):284-289.doi:10.1038/s41586-020-2947-8
9. Walkowiak S, Gao L, Monat C, et al.Awọn genomes alikama pupọ ṣafihan iyatọ agbaye ni ibisi igbalode.Iseda.2020;588(7837):277-283.doi:10.1038/s41586-020-2961-x
10. Tao Y, Luo H, Xu J, et al.Iyatọ ti o gbooro laarin pan-genome ti gbin ati oka igbẹ [ti a tẹjade lori ayelujara ṣaaju titẹ, 2021 May 20].Awọn ohun ọgbin Nat.2021;10.1038 / s41477-021-00925-x.doi:10.1038/s41477-021-00925-x
11. Fan X, Qiu H, Han W, et al.Phytoplankton pangenome ṣe afihan gbigbe jiini petele prokaryotic lọpọlọpọ ti awọn iṣẹ oniruuru.Sci Adv.2020;6(18):eaba0111.Atejade 2020 Oṣu Kẹrin Ọjọ 29. doi:10.1126/sciadv.aba0111
Akoko ifiweranṣẹ: Jan-04-2022