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Epigenetics

  • Chromatin Immunoprecipitation Sequencing (ChIP-seq)

    Ilana Isọju Ajẹsara Chromatin (ChiP-seq)

    ChIP-Seq n pese profaili jiini jakejado ti awọn ibi-afẹde DNA fun iyipada itan-akọọlẹ, awọn ifosiwewe transcription, ati awọn ọlọjẹ miiran ti o ni ibatan DNA.O daapọ yiyan ti chromatin immuno-precipitation (ChiIP) lati gba awọn ile-iṣẹ amuaradagba-DNA kan pato pada, pẹlu agbara ti atẹle-iran ti o tẹle (NGS) fun tito-ọna-giga ti DNA ti o gba pada.Ni afikun, nitori awọn ile-iṣẹ amuaradagba-DNA ti gba pada lati awọn sẹẹli alãye, awọn aaye asopọ le ṣe afiwe ni oriṣiriṣi awọn iru sẹẹli ati awọn tisọ, tabi labẹ awọn ipo oriṣiriṣi.Awọn ohun elo wa lati ilana transcriptional si awọn ipa ọna idagbasoke si awọn ilana arun ati kọja.

    Syeed: Illumina NovaSeq 6000

  • Whole genome bisulfite sequencing

    Gbogbo genome bisulfite titele

    DNA methylation ni ipo karun ni cytosine (5-mC) ni ipa ipilẹ lori ikosile pupọ ati iṣẹ ṣiṣe cellular.Awọn ilana methylation ajeji ti ni nkan ṣe pẹlu ọpọlọpọ awọn ipo ati awọn arun, gẹgẹbi akàn.WGBS ti di boṣewa goolu fun kikọ ẹkọ methylation jakejado-genome ni ipinnu ipilẹ ẹyọkan.

    Syeed: Illumina NovaSeq6000

  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

    Igbeyewo fun Transposase-Wiwọle Chromatin pẹlu Atẹle Gbigbe Giga (ATAC-seq)

    ATAC-seq jẹ ọna itọsẹ-giga ti o ga fun itupalẹ iraye si chromatin jakejado-genome, eyiti o ṣe pataki fun iṣakoso epigenetic agbaye ti ikosile pupọ.Awọn oluyipada ilana ti a fi sii si awọn agbegbe chromatin ti o ṣii nipasẹ transposase Tn5 hyperactive.Lẹhin imudara PCR, ile-ikawe itẹlera kan ti kọ.Gbogbo awọn agbegbe chromatin ti o ṣii ni a le gba labẹ ipo aaye-akoko kan pato, kii ṣe opin nikan si awọn aaye abuda ti ifosiwewe transcription, tabi agbegbe kan ti a ti yipada itan-akọọlẹ kan.

  • Reduced Representation Bisulfite Sequencing (RRBS)

    Idinku Aṣoju Bisulfite Sequencing (RRBS)

    Iwadi methylation DNA ti jẹ koko-ọrọ ti o gbona nigbagbogbo ninu iwadii aisan, ati pe o ni ibatan pẹkipẹki si ikosile pupọ ati awọn ami-ara-pheno.RRBS jẹ ọna ti o peye, daradara ati ti ọrọ-aje fun iwadii DNA methylation.Imudara ti olupolowo ati awọn agbegbe erekusu CpG nipasẹ enzymatic cleavage (Msp I), ni idapo pẹlu ilana ilana Bisulfite, pese wiwa methylation DNA ti o ga.

    Syeed: Illumina NovaSeq 6000

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