BMKMANU S1000 Spatial Transcriptome
BMKMANU S1000 Spatial Transcriptome Technical Ero
Awọn anfani ti BMKMANU S1000
1) Ipinnu iha-cellular: Agbegbe gbigba kọọkan ni>2 million awọn aaye Barcoded aaye pẹlu iwọn ila opin kan ti 2.5 µm ati aye kan ti 5 µm laarin awọn ile-iṣẹ iranran, ti n muu ṣiṣẹ itupalẹ transcriptome aye pẹlu ipinnu sub-cellular (5 μm).
2) Itupalẹ ipinnu ipele-ọpọlọpọ: Itupalẹ ipele pupọ ti o rọ lati 100 μm si 5 μm lati yanju awọn ẹya ara ẹrọ oniruuru ni ipinnu to dara julọ.
3) Ifitonileti iwe-kikọ ti o ni kikun: Awọn iwe afọwọkọ ti o gba lati gbogbo ifaworanhan tissu le ṣe itupalẹ, laisi ihamọ lori nọmba awọn jiini ibi-afẹde ati agbegbe ibi-afẹde.
Awọn pato Iṣẹ
| Ile-ikawe | Ilana titele | Ijade data niyanju |
| S1000 cDNA ìkàwé | BMKMANU S1000-Illumina PE150 | 60Gb/apẹẹrẹ |
Awọn ibeere apẹẹrẹ
| Apeere | Nọmba | Iwọn | Didara RNA |
| OCT ifibọ àsopọ Àkọsílẹ | 2-3 ohun amorindun / apẹẹrẹ | Isunmọ.6.8x6.8x6.8 mm3 | RIN≥7 |
Fun awọn alaye diẹ sii lori itọsọna igbaradi ayẹwo ati ṣiṣan iṣẹ iṣẹ, jọwọ lero ọfẹ lati ba aBMKGENE iwé
Sisan Iṣẹ Iṣẹ
Apẹrẹ adanwo
Ifijiṣẹ apẹẹrẹ
Ṣaju idanwo
Aaye kooduopo
Ikole ìkàwé
Titele
Itupalẹ data
Lẹhin-tita awọn iṣẹ
Awọn data ti ipilẹṣẹ nipasẹ BMKMANU S1000 jẹ atupale nipa lilo sọfitiwia “BSTMatrix”, eyiti o jẹ apẹrẹ ni ominira nipasẹ BMKGENE, pẹlu:
1) Gene ikosile matrix iran
2) HE image processing
3) Ni ibamu pẹlu sọfitiwia ẹni-kẹta ibosile fun itupalẹ
4) Online “BSTViewer” ṣe iranlọwọ lati gba awọn abajade iworan ni awọn ipinnu oriṣiriṣi.
1.Spot iṣupọ
2.Spatial pinpin
Note: ipinnuipele=13 (100 µm, osi); 7 (50 µm, otun)
3.Marker ikosile opo clustering heatmap
4.Inter-sample data onínọmbà
