Awọn kokoro arun pipe Genome
Awọn anfani Iṣẹ
● Awọn ilana ilana ilana pupọ ti o wa fun awọn ibi-afẹde iwadii oriṣiriṣi
● Awọn kokoro arun pipe jiini pẹlu 0 Gap ẹri.
● Ni iriri giga ni apejọ genome kokoro arun pẹlu diẹ sii ju 10,000 ti o pejọ.
● Ọjọgbọn lẹhin-tita atilẹyin imọ-ẹrọ egbe nmu awọn ibeere iwadi pato diẹ sii.
Awọn pato Iṣẹ
| TiteleIlana | Ile-ikawe | Didara ẹri | Ifoju-yika akoko |
| Nanopore 100X + Illumina 50X | Nanopore 20K PE150 | 0 Aafo | 30 Ọjọ |
| PacBio HiFi 30X | PacBio 10K |
Awọn itupalẹ bioinformatics
● Aise data didara iṣakoso
● Apejọ Genome
● Ayẹwo paati Jinom
● Atọka iṣẹ Jiini
● Ayẹwo genomic afiwera
Awọn ibeere Ayẹwo ati Ifijiṣẹ
Awọn ibeere Apeere:
FunDNA ayokuro:
| Apeere Iru | Iye | Ifojusi | Mimo |
| DNA ayokuro | 2 μg | 20ng/μl | OD260/280 = 1.6-2.5 |
Fun awọn ayẹwo iṣan:
| Iru apẹẹrẹ | Itọju ayẹwo ti a ṣe iṣeduro | Ayẹwo ipamọ ati sowo |
| Awọn kokoro arun | Ṣe akiyesi awọn kokoro arun labẹ maikirosikopu ki o gba awọn kokoro arun ni ipele ipari wọn Gbigbe aṣa kokoro arun (ti o ni isunmọ. 3-4.5e9 awọn sẹẹli) sinu eppendorf 1.5 tabi 2 milimita.(Tẹ lori yinyin) Centrifuge tube fun iṣẹju 1 ni 14000 g lati gba awọn kokoro arun ati yọọ supernatant ni pẹkipẹki. Pa tube naa ki o di awọn kokoro arun sinu nitrogen olomi fun o kere 30 min.Tọju tube ni -80 ℃ firiji. | Di awọn ayẹwo naa sinu omi nitrogen fun awọn wakati 3-4 ati fipamọ sinu nitrogen olomi tabi iwọn -80 si ifiṣura igba pipẹ.Ayẹwo gbigbe pẹlu yinyin gbigbẹ ni a nilo. |
Sisan Iṣẹ Iṣẹ
Ifijiṣẹ apẹẹrẹ
Ikole ìkàwé
Titele
Itupalẹ data
Lẹhin-tita awọn iṣẹ
1.Circos ti kokoro arun genome
2.Coding pupọ asọtẹlẹ
3.Comparative genomics onínọmbà: phylogenetic igi
