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条形pasia-03

Awọn ọja

Igbeyewo fun Transposase-Wiwọle Chromatin pẹlu Atẹle Gbigbe Giga (ATAC-seq)

Iwadii fun Transposase-Wiwọle Chromatin pẹlu Tito-ọna Gbigbe Giga (ATAC-seq) Aworan Iṣafihan
  • Igbeyewo fun Transposase-Wiwọle Chromatin pẹlu Atẹle Gbigbe Giga (ATAC-seq)
  • Igbeyewo fun Transposase-Wiwọle Chromatin pẹlu Atẹle Gbigbe Giga (ATAC-seq)
  • Igbeyewo fun Transposase-Wiwọle Chromatin pẹlu Atẹle Gbigbe Giga (ATAC-seq)
  • Igbeyewo fun Transposase-Wiwọle Chromatin pẹlu Atẹle Gbigbe Giga (ATAC-seq)
  • Igbeyewo fun Transposase-Wiwọle Chromatin pẹlu Atẹle Gbigbe Giga (ATAC-seq)

ATAC-seq jẹ ọna itọsẹ-giga ti o ga fun itupalẹ iraye si chromatin jakejado-genome, eyiti o ṣe pataki fun iṣakoso epigenetic agbaye ti ikosile pupọ.Awọn oluyipada ti o tẹle ni a fi sii si awọn agbegbe chromatin ti o ṣii nipasẹ transposase Tn5 hyperactive.Lẹhin imudara PCR, ile-ikawe ti o tẹle ni a ṣe.Gbogbo awọn agbegbe chromatin ti o ṣii ni a le gba labẹ ipo aaye-akoko kan pato, kii ṣe opin nikan si awọn aaye abuda ti ifosiwewe transcription, tabi agbegbe kan ti a ti yipada itan-akọọlẹ kan.


Awọn alaye Iṣẹ

Bioinformatics

Awọn abajade Ririnkiri

Awọn anfani Iṣẹ

● Itupalẹ Okeerẹ: Opo opo gigun ti oye ọjọgbọn lati pade ọpọlọpọ awọn iwulo onínọmbà ti adani;

● Iriri ti o pọju: Awọn ọgọọgọrun awọn iṣẹ akanṣe ti pari ni aṣeyọri titi di isisiyi, pẹlu ẹranko ati awọn apẹẹrẹ ọgbin.

Awọn pato Iṣẹ

Platform: Illumina NovaSeq Syeed

Iru ikawe: ATAC-seq

Ijade data ti a ṣe iṣeduro: ≥20M kika

Awọn ibeere apẹẹrẹ

Iru apẹẹrẹ: Awọn sẹẹli, awọn sẹẹli laaye, tabi awọn sẹẹli tio tutunini ati bẹbẹ lọ

Nọmba alagbeka: ≥ 5×105awọn sẹẹli, nọmba sẹẹli deede jẹ bọtini si aṣeyọri ti idanwo naa;

Iwọn ti ara: ≥ 200mg alabapade;

Ẹjẹ: ≥ 2 milimita

Sisan Iṣẹ Iṣẹ

Ayẹwo QC

Apẹrẹ adanwo

ifijiṣẹ ayẹwo

Ifijiṣẹ apẹẹrẹ

Pilot ṣàdánwò

DNA isediwon

Igbaradi Library

Ikole ìkàwé

Titele

Titele

Itupalẹ data

Itupalẹ data

Lẹhin ti sale Services

Lẹhin-tita awọn iṣẹ

  • Ti tẹlẹ:
  • Itele:

    • 表观-02

    1.Heatmap lori ATAC ka pinpin ni TSS ati awọn agbegbe ti o wa nitosi (± 3 kb)

    3 (1)2.Pinpin ti agbegbe chromatin ti o ṣii ni awọn oriṣiriṣi awọn ẹya-ara genome

    4 (1)

    3.Differential tente oke pipe laarin awọn ẹgbẹ

    5 (1)

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