Iqonga: iqonga leNovaSeq, PE150
Uhlobo lwethala leencwadi: 200-400bp faka i-bisulfite enyangwa kwithala leencwadi le-DNA
Isicwangciso sokulandelelana: I-Paired-End 150 bp
Cebisa imveliso yedatha: 10 Gb idatha ekrwada / isampuli
DNA ubuninzi: ≥ 2ug
Ugxininiso lweDNA:≥ 20ng/μl.
Ubunyulu: OD260/280 = 1.8 ukuya ku-2.0 ngaphandle kokuthotywa okanye ukungcoliswa kwe-RNA
a.Iinkcukacha-manani zolungelelwaniso lwe-genome
SIzibalo zobunzulu bolandelelwano kunye nokugubungela indawo yeC
Aukhuselo oluqokelelweyo
Dukusebenza kakuhle kwesampulu nganye
b.Ukufunyanwa kwendawo ye-methylation
Uluhlu lwamanqanaba e-methylation kwindawo ye-C
BS ukuguqulwa
Ukuhanjiswa kwenqanaba le-methylation
c.Imephu ye-methylation
Genome element
d.Ukuthelekiswa kwamanqanaba e-methylation phakathi kweesampuli
Pemoyeni Unxulumano lwe-CG methylation
d.Uhlalutyo lwe-methylation ngokwahlukileyo
SIinkcukacha-manani zeDMR