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条形 ibhanile-03

Iimveliso

Umekelo oluNcitshisiweyo lweBisulfite Sequencing (RRBS)

Uphando lwe-DNA methylation luhlala luyisihloko esishushu kuphando lwezifo, kwaye luhambelana ngokusondeleyo ne-gene expression kunye ne-pheno-typic traits.I-RRBS yindlela echanekileyo, esebenzayo kunye neyoqoqosho yophando lwe-DNA methylation.Ukutyetyiswa komgqugquzeli kunye nemimandla yesiqithi se-CpG nge-enzymatic cleavage (Msp I), edityaniswe nokulandelelana kwe-Bisulfite, ibonelela ngesisombululo esiphezulu se-DNA methylation.

Iqonga: I-Illumina NovaSeq Platform


Iinkcukacha zeNkonzo

Iziphumo zeDemo

IiNgcaciso zeNkonzo

Iqonga: iqonga leNovaSeq, PE150

Uhlobo lwethala leencwadi: 200-400bp faka i-bisulfite enyangwa kwithala leencwadi le-DNA

Isicwangciso sokulandelelana: I-Paired-End 150 bp

Cebisa imveliso yedatha: 10 Gb idatha ekrwada / isampuli

IiMfuno zeSampulu

DNA ubuninzi: ≥ 2ug

Ugxininiso lweDNA:≥ 20ng/μl.

Ubunyulu: OD260/280 = 1.8 ukuya ku-2.0 ngaphandle kokuthotywa okanye ukungcoliswa kwe-RNA

Uhlalutyo lwe-Bioinformatics

umfanekiso weskrini 4-03

Ukuhamba komsebenzi weNkonzo

ukuhanjiswa kwesampuli

Ukuhanjiswa kwesampuli

ULungiselelo lweThala leeNcwadi

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Ukulandelelanisa

Uhlalutyo lwedatha

Uhlalutyo lwedatha

Emva kweeNkonzo zentengiso

Iinkonzo emva kokuthengisa


  • Ngaphambili:
  • Okulandelayo:

  • a.Iinkcukacha-manani zolungelelwaniso lwe-genome

    Ubalo lwemephu lufundeka
    umfanekiso3

    SIzibalo zobunzulu bolandelelwano kunye nokugubungela indawo yeC

    umfanekiso4

    Aukhuselo oluqokelelweyo

    umfanekiso5

    Dukusebenza kakuhle kwesampulu nganye

    umfanekiso6

    b.Ukufunyanwa kwendawo ye-methylation

    Uluhlu lwamanqanaba e-methylation kwindawo ye-C

    umfanekiso 7

    BS ukuguqulwa

    umfanekiso8 umfanekiso 9

    Methylated cytosine
    umfanekiso10

    Ukuhanjiswa kwenqanaba le-methylation
    umfanekiso11

    c.Imephu ye-methylation

    Genome-banzi
    umfanekiso12

    Genome element

    umfanekiso13

    d.Ukuthelekiswa kwamanqanaba e-methylation phakathi kweesampuli
    umfanekiso14

    Pemoyeni Unxulumano lwe-CG methylation
    umfanekiso15

    PI-CA-CG methylation
    umfanekiso16

    CUkukhanya kwe-CG methylation
    umfanekiso17

    d.Uhlalutyo lwe-methylation ngokwahlukileyo

    SIinkcukacha-manani zeDMR

    umfanekiso18

    Ukuhanjiswa kobude be-DMR
    umfanekiso19

    Hyitya imephu
    umfanekiso20

    DUkusasazwa kwe-MR kwindawo ekujoliswe kuyo
    umfanekiso21

    fumana isicatshulwa

    Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    Thumela umyalezo wakho kuthi: