Iimveliso

Prokaryotic RNA ulandelelwano

Ukulandelelana kwe-Prokaryotic RNA kusebenzisa isizukulwana esilandelayo sokulandelelana (NGS) ukuveza ubukho kunye nobuninzi be-RNA ngexesha elithile, ngokuhlalutya ukuguqulwa kwe-transcriptome yeselula.Ulandelelwano lwe-RNA ye-prokaryotic yenkampani yethu, ijolise ngokukodwa kwiiprokaryotes ezine-reference genomes, ikubonelela ngeprofayili ye-transcriptome, uhlalutyo lwesakhiwo sofuzo, njl njl.

Iqonga: I-Illumina NovaSeq Platform

Qhagamshelana nathi

Iinkcukacha zeNkonzo

IiNgcaciso zeNkonzo

Iqonga: Illumina NovaSeq iqonga, PE150

Uhlobo lwethala leencwadi: 350bp faka ithala leencwadi le-cDNA (kuxhomekeke kwincopho yosasazo)

Isicwangciso sokulandelelana: I-Paired-End 150 bp

Cebisa isixa sedatha: 2 Gb idatha ekrwada/isampulu

Bioinformatics

(1) Ukulandelelanisa ulawulo lomgangatho wedatha: Ukuhanjiswa kwe-Nucleotide, ukuhanjiswa komgangatho weSiseko, uvavanyo lomlinganiselo we-rRNA;

(2) Uhlalutyo lolungelelwaniso lolandelelwano: Iinkcukacha-manani zeziphumo zolungelelwaniso, Ukugcwaliswa kolandelelwano, ukuFumana ngokulandelelana;

(3) Isalathisi se-genome (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Uhlalutyo lwenkcazo: Ukusabalalisa ukubonakaliswa (Ngeesampuli ezimbini okanye ngaphezulu), Uhlalutyo lwentetho phakathi kweesampuli (uhlalutyo lwe-Venn, uhlalutyo lokulungelelanisa, uhlalutyo lwe-PCA);

(5) Uhlalutyo lwentetho eyahlukileyo (Ngeesampuli ezimbini okanye ngaphezulu);

(6) Uhlalutyo lweseti ye-Gene: Uhlalutyo lwe-Venn, uhlalutyo lwe-Cluster, uhlalutyo lwe-Function annotation (COG, GO, KEGG), uhlalutyo lwe-Function enrichment (GO, KEGG), i-Function enrichment chordal graph, uhlalutyo lwe-Ipath;

(7) Uhlalutyo lwe-sRNA: ukubikezelwa kwe-sRNA, i-sRNA inkcazo (i-Blast, i-sRNAMAp, i-sRNATarBase, i-SIPHT, i-Rfam), i-sRNA i-prediction yesakhiwo sesibini, i-sRNA ekujoliswe kuyo kwi-gene ye-gene;

(8) Uhlalutyo lwesakhiwo se-Transcript: Uhlalutyo lwe-Operon, i-Transcription yokuqala kunye nokuphela kweendawo zokubikezela, i-UTR inkcazo kunye nohlalutyo, i-Promoter sequence prediction, uhlalutyo lwe-SNP / InDel (i-SNP / InDel annotation, i-SNP / InDel yokusabalalisa kwingingqi, izibalo ze-SNP).