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BSA
Iqonga le-Bulked Segregant Analysis iquka uhlalutyo olusemgangathweni lwenyathelo elinye kunye nohlalutyo oluphambili kunye nokumisela ipharamitha elungiselelwe.I-BSA bubuchule obusetyenziselwa ukuchonga ngokukhawuleza iimpawu zemfuzo ezinxulumene nephenotype.Umsebenzi ongundoqo we-BSA uqulathe: 1. ukukhetha amaqela amabini abantu abaneephenotypes ezichasene kakhulu;2. ukudibanisa i-DNA, i-RNA okanye i-SLAF-seq(Iphuhliswe yi-Biomarker) yabo bonke abantu ukuze benze isambuku esibini se-DNA;3. ukuchonga ulandelelwano lokwahlula ngokuchasene ne-reference genome okanye phakathi, 4. ukuqikelela umviwa onxibelelene nemimandla nge-ED kunye ne-SNP-index algorithm;5. Ucazululo olusebenzayo kunye nophuculo kwiijini kwimimandla yabaviwa, njl. njl. Ukumbiwa kwedatha okuqhubekeke ngakumbi kubandakanywa ne-genetic marker screening kunye ne-primer design nazo ziyafumaneka.
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I-Amplicon (16S/18S/ITS)
Iqonga le-Amplicon (16S / 18S / ITS) liphuhliswa kunye neminyaka yamava kuhlalutyo lweprojekthi ye-microbial diversity, equlethe uhlalutyo olusisiseko olusemgangathweni kunye nohlalutyo lomntu siqu: uhlalutyo olusisiseko lubandakanya umxholo wohlalutyo oluqhelekileyo lophando lwangoku lwe-microbial, umxholo wokuhlalutya ucebile kwaye ubanzi, kunye neziphumo zohlalutyo zinikezelwe ngohlobo lweengxelo zeprojekthi;Umxholo wohlalutyo lobuqu uhlukile.Iisampulu zinokukhethwa kwaye iiparameters zinokumiselwa ngokuguquguqukayo ngokwengxelo yohlalutyo olusisiseko kunye nenjongo yophando, ukufezekisa iimfuno zomntu.Inkqubo yokusebenza yeWindows, ilula kwaye iyakhawuleza.
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I-Evolutionary Genetics
I-Evolutionary genetics yinkonzo yolandelelwano epakishweyo eyenzelwe ukunika utoliko olubanzi kulwazi lwendaleko lwemathiriyeli enikeziweyo esekelwe kwiiyantlukwano zemfuza, kubandakanywa ii-SNP, i-InDels, i-SVs kunye ne-CNVs.Ibonelela ngalo lonke ucazululo olusisiseko olufunekayo ekuchazeni utshintsho lwendaleko kunye neempawu zemfuza zabantu, ezifana nokwakheka kwabantu, iyantlukwano yemfuza, ubudlelwane bephylogeny, njl.
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Ukuthelekisa iGenomics
Ukuthelekisa i-genomics ngokoqobo kuthetha ukuthelekisa ulandelelwano olupheleleyo lwe-genome kunye nezakhiwo zeentlobo ezahlukeneyo.Olu qeqesho lujolise ekubonakaliseni ukuvela kweentlobo, umsebenzi wemfuza, indlela yokulawula imfuza kwinqanaba le-genome ngokuchonga izakhiwo zolandelelwano kunye nezinto ezigciniweyo okanye ezahlulayo kwiintlobo ezahlukeneyo zeentlobo.Uphononongo oluqhelekileyo lothelekiso lwe-genomics lubandakanya ucazululo kusapho lwemfuza, uphuhliso lwendaleko, uphindaphindo lwegenome epheleleyo, uxinzelelo olukhethiweyo, njl.njl.
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I-Evolutionary Genetics
Inani labantu kunye neqonga lokuhlalutya imfuzo lisekwe kwisiseko samava amakhulu aqokelelwe kwiqela le-BMK R&D iminyaka.Sisixhobo esisebenziseka lula ngakumbi kubaphandi abangabalulekanga kwi-bioinformatics.Eli qonga livumela uhlalutyo olusisiseko olunxulumene nofuzo olusisiseko olubandakanya ukwakhiwa komthi we-phylogenetic, uhlalutyo lwe-disequilibrium yonxibelelwano, uvavanyo lokwahluka kwemfuza, uhlalutyo olukhethiweyo lokutshayela, uhlalutyo lokuzalana, i-PCA, uhlalutyo lwesakhiwo sabemi, njl.
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INdibano yeGenome esekwe kwi-Hi-C
I-Hi-C yindlela eyilelwe ukubamba ulungelelwaniso lwechromosome ngokudibanisa ukudibana okusekwe kufutshane kunye nolandelelwano oluphezulu.Ukuqina kolu nxibelelwano kukholelwa ukuba lunxulunyaniswa kakubi nomgama womzimba kwiichromosomes.Ke ngoko, idatha ye-Hi-C inokukhokela ukudityaniswa, ukulandelelana kunye nokulungelelaniswa kolandelelwano oludityanisiweyo kwi-genome eyidrafti kunye nokubethelela ezo kwinani elithile lekhromozomi.Obu buchwepheshe buxhobisa indibano yegenome yenqanaba lechromosome ngokungabikho kwemephu yemfuzo esekwe ngabantu.Yonke i-genome enye idinga i-Hi-C.
Iqonga: I-Illumina NovaSeq Platform / DNBSEQ
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Isityalo/Isilwanyana saseDe Novo Genome Sequencing
De Novoulandelelwano lubhekisa kulwakhiwo lohlobo lwe-genome epheleleyo kusetyenziswa ubugcisa bokulandelelana, umz. i-PacBio, i-Nanopore, i-NGS, njl.Uphuculo oluphawulekayo kubude bokufunda besizukulwana sesithathu sobuchwephesha bokulandelelana buye bazisa amathuba amatsha ekuhlanganiseni i-genomes entsonkothileyo, njengalezo ezine-heterozygosity ephezulu, umlinganiselo ophezulu wemimandla ephindaphindwayo, iipolyploids, njl. ukusombulula izinto eziphindaphindwayo, imimandla enomxholo we-GC ongaqhelekanga kunye neminye imimandla entsonkothileyo kakhulu.
Iqonga: I-PacBio Sequel II / Nanopore PromethION P48 / Illumina NovaSeq Platform
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Ulandelelwano lwe-Exome yoMntu
I-Whole exome sequencing (i-WES) ithathwa njengesicwangciso esiliqili sokulandelelanisa iindleko zokuchonga iinguqu ezibangela izifo.Nangona i-exons ithatha kuphela malunga ne-1.7% ye-genome epheleleyo, imele iprofayili yemisebenzi yeprotheyini ngokuthe ngqo.Kwi-genome yomntu, kuye kwaxelwa ukuba ngaphezulu kwe-85% yotshintsho olunxulumene nesifo lwenzeka kwindawo yekhowudi yeprotheyini.
I-BMKGENE inikezela ngeenkonzo ezibanzi neziguquguqukayo zolandelelwano lwabantu ngokubanzi kunye neendlela ezahlukeneyo zokubamba ze-exon ezikhoyo ukuhlangabezana neenjongo ezahlukeneyo zophando.
Iqonga: I-Illumina NovaSeq Platform
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I-Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
I-genotyping ephezulu, ngokukodwa kwinani elikhulu labantu, linyathelo elisisiseko kwizifundo zombutho wemfuza, obonelela ngesiseko sofuzo sofuzo olusebenzayo, uhlalutyo lwe-evolution, njl. Endaweni yolandelelwano olunzulu lwe-genome kwakhona, ukunciphisa ukumelwa kwe-genome sequencing (RRGS). ) yaziswa ukuze kuncitshiswe iindleko zolandelelwano ngesampulu nganye, ngeli lixa kugcinwe ukusebenza kakuhle ekufumaneni uphawu lwemfuzo.Oku kudla ngokuphunyezwa ngokutsala isiqwenga sothintelo ngaphakathi koluhlu lobungakanani obunikiweyo, olubizwa ngokuba yithala leencwadi lokumelwa elincitshisiweyo (RRL).I-Specific-locus amplified fragment sequencing (SLAF-Seq) sisicwangciso esiziphuhlisileyo se-SNP genotyping kunye okanye ngaphandle kwe-genome yesalathiso.
Iqonga: I-Illumina NovaSeq Platform -
Illumina kunye ne-BGI
Itekhnoloji yolandelelwano ye-Illumina, esekwe kulandelelwano nge-Synthesis (SBS), yinto entsha ye-NGS eyamkelwe kwihlabathi jikelele, enoxanduva lokuvelisa ngaphezulu kwe-90% yedatha yolandelelwano lwehlabathi.Umthetho-siseko we-SBS ubandakanya umboniso we-fluorescent obhalwe izitermina ezibuyiselwa umva njengoko i-dNTP nganye yongezwa, kwaye emva koko yacandwa ukuvumela ukufakwa kwesiseko esilandelayo.Ngazo zone ii-dNTP ezine-terminator-bound bounded dNTP ezikhoyo kumjikelo ngamnye wolandelelwano, ukhuphiswano lwendalo lunciphisa ukubandakanywa kokubandakanya.Ubuchwephesha obuguquguqukayo buxhasa amathala eencwadi afundwe kunye kunye nesiphelo sesibini, silungiselela uluhlu lwezicelo ze-genomic.I-Illumina yokulandelanisa i-high-throughput i-high-throughput ibeka njengelitye lembombo kuphando lwe-genomics, ixhobisa izazinzulu ukuba ziveze ubucukubhede be-genomes ngeenkcukacha ezingenakulinganiswa kunye nokusebenza kakuhle.
I-DNBSEQ, ephuhliswe yi-BGI, yenye itekhnoloji ye-NGS ekwaziyo ukuhla ngakumbi kwiindleko zolandelelwano kunye nokunyusa umthamo.Ukulungiswa kweelayibrari ze-DNBSEQ kubandakanya ukuhlukana kwe-DNA, ukulungiswa kwe-ssDNA kunye nokukhulisa isangqa esijikelezayo ukufumana i-nanoballs ye-DNA (DNB).Ezi ke ziye zilayishwe kumphezulu oqinileyo kwaye emva koko zilandelelaniswe ngokudityaniswa kweProbe-Anchor Synthesis (cPAS).
Inkonzo yethu yolandelelwano yethala leencwadi eyenziwe kwangaphambili iququzelela abathengi ekulungiseleleni iilayibrari zabo ezilandelelanayo ezivela kwimithombo eyahlukeneyo (mRNA, genome yonke, amplicon, phakathi kwabanye).Emva koko, la mathala eencwadi anokuthunyelwa kumaziko ethu okulandelelanisa ulawulo lomgangatho kunye nokulandelelana kwiiplatifti ze-Illumina okanye ze-BGI.
