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Ulandelelwano lweMetatranscriptome
Ulandelelwano lweMetatranscriptome luchonga ukubonakaliswa kofuzo lwemicrobes (zombini ieukaryotes kunye neprokaryotes) ngaphakathi kokusingqongileyo kwendalo (oko kukuthi, umhlaba, amanzi, ulwandle, ilindle, kunye namathumbu.) Ngokukodwa, ezi nkonzo zikuvumela ukuba ufumane iprofayili yegenesis epheleleyo yeentsholongwane ezintsonkothileyo, uhlalutyo lwetaxonomic. kweentlobo, uhlalutyo lokutyebisa olusebenzayo lwemfuza echazwe ngokwahlukileyo, kunye nokunye.
Iqonga: I-Illumina NovaSeq Platform
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I-Fungal Genome
I-Biomarker Technologies ibonelela ngesaveyi ye-genome, i-genome entle kunye ne-pene-complete genome ye-fungal ngokuxhomekeke kwinjongo ethile yophando.Ukulandelelana kweGenome, indibano kunye nenkcazo esebenzayo inokufezekiswa ngokudibanisa isizukulwana esilandelayo + Ukulandelelana kwesizukulwana sesithathu ukuphumeza indibano yegenome yenqanaba eliphezulu.Itekhnoloji ye-Hi-C inokuqeshwa ukuququzelela ukudibanisa i-genome kwinqanaba le-chromosome.
Iqonga:I-PacBio Sequel II
Nanopore PromethION P48
I-Illumina NovaSeq Platform
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Iikhithi zezixhobo
I-BMKCloud yiqonga elikhokelayo le-bioinformatic elibonelela ngesisombululo esisodwa kwiinkqubo ze-genomic, ezithenjwa ngokubanzi ngabaphandi kwiindawo ezahlukeneyo ezibandakanya ezonyango, ezolimo, ezendalo, njl. , izixhobo zekhompyutha, isiseko sedatha yoluntu, izifundo ze-intanethi ze-bioinformatiki, njl. njl. I-BMKCloud inezixhobo ezahlukeneyo ezisetyenziswa rhoqo ze-bioinformatic ezibandakanya i-gene annotation, izixhobo ze-evolutionary genetic, i-ncRNA, ulawulo lomgangatho wedatha, ukudibanisa, ukulungelelaniswa, ukutsalwa kwedatha, ukuguqulwa kwedatha, izibalo, umvelisi wamanani, ulandelelwano. uhlalutyo, njl.
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Iintsholongwane ziGqibezela iGenome
I-Biomarker Technologies ibonelela ngenkonzo yolandelelwano ekwakheni i-genome epheleleyo yebhaktiriya ene-gap engu-zero.Ukuhamba komsebenzi okuphambili kwebhaktheriya ukwakhiwa okupheleleyo kwegenome kubandakanya ulandelelwano lwesizukulwana sesithathu, indibano, inkcazo esebenzayo kunye nohlalutyo oluphambili lwe-bioinformatiki oluzalisekisa iinjongo ezithile zophando.Iprofayili ebanzi ngakumbi yeebhaktiriya genome ixhobisa ukutyhilwa kweendlela ezisisiseko ezisisiseko senkqubo yazo yebhayoloji, enokubonelela ngereferensi ebalulekileyo kuphando lwegenomic kwiintlobo eziphezulu ze-eukaryotic.
Iqonga:Nanopore PromethION P48 + Illumina NovaSeq Platform
I-PacBio Sequel II
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iRNA encinci
Ii-RNA ezincinci luhlobo lwe-RNA emfutshane engeyiyo ikhowudi kunye nobude obuphakathi kwe-18-30 nt, kuquka i-miRNA, i-siRNA kunye ne-piRNA.Ezi RNA zincinci ziye zaxelwa kakhulu ukuba zibandakanyeka kwiinkqubo ezahlukeneyo zebhayoloji ezifana nokuthotywa kwe-mRNA, inhibition yokuguqulela, ukubunjwa kwe-heterochromatin, njl njl. Uhlalutyo lokulandelelana kwe-SmallRNA lusetyenziswe ngokubanzi kwizifundo zokuphuhliswa kwezilwanyana / izityalo, izifo, intsholongwane, njl njl. iqonga lokucazulula ngokulandelelana liquka uhlalutyo olusemgangathweni kunye nokumbiwa kwedatha ephezulu.Kwisiseko sedatha ye-RNA-seq, uhlalutyo olusemgangathweni lunokufikelela ukuchongwa kunye noqikelelo lwe-miRNA, uqikelelo lwemfuza ekujoliswe kuyo yi-miRNA, inkcazo kunye nohlalutyo lwentetho.Uhlalutyo oluphezulu luvumela ukukhangela kunye nokutsalwa kwe-miRNA ngokwezifiso, ukuveliswa komzobo weVenn, i-miRNA kunye nolwakhiwo lwenethiwekhi yemfuza ekujoliswe kuyo.
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I-NGS-WGS (Illumina/BGI)
I-NGS-WGS liqonga lokuhlalutya lokulandelelana kwakhona kwe-genome, eliphuhliswe kwisiseko samava atyebileyo kwi-Biomarker Technologies.Eli qonga lisebenziseka lula livumela ukungeniswa okukhawulezayo kokuhamba komsebenzi wokuhlalutya okudibeneyo ngokubeka nje iparameter embalwa esisiseko, ehambelana nedatha yolandelelwano yeDNA eyenziwe kwiqonga le-Illumina kunye neqonga lokulandelelana kwe-BGI.Eli qonga libekwe kwiseva ephezulu yokusebenza kwekhompyuter, exhobisa uhlalutyo lwedatha olusebenzayo ngexesha elincinci kakhulu.Ukumbiwa kwedatha yobuqu kufumaneka kwisiseko sohlalutyo olusemgangathweni, kubandakanywa nombuzo oguquliweyo we-gene, uyilo lwe-PCR primer, njl.
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mRNA(Reference)
I-Transcriptome likhonkco phakathi kolwazi lwemfuzo ye-genomic kunye neproteome yomsebenzi webhayoloji.Ummiselo wenqanaba lokukhutshelwa ngowona ubalulekileyo kwaye yeyona ndlela yolawulo efundwa ngokubanzi yezinto eziphilayo.Ulandelelwano lwe-Transcriptome luyakwazi ukulandelelanisa i-transcriptome nakweyiphi na indawo ngexesha okanye phantsi kwayo nayiphi na imeko, kunye nesisombululo esichanekileyo kwi-nucleotide enye. isampula yemibhalo ebhaliweyo ethile.
Okwangoku, itekhnoloji yokulandelelanisa i-transcriptome isetyenziswe ngokubanzi kwi-agronomy, iyeza kunye nezinye iindawo zophando, kubandakanywa ukulawulwa kokuphuhliswa kwezilwanyana kunye nezityalo, ukulungelelaniswa kwendalo, ukusebenzisana kwe-immune, i-gene localization, iintlobo ze-genetic evolution kunye ne-tumor kunye nokufumanisa isifo sofuzo.
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IMetagenomics (NGS)
Eli qonga lokuhlalutya lenzelwe ukudubula kwedatha ye-metagenomic kwisiseko seminyaka yamava.Iquka ukuhamba komsebenzi okudityanisiweyo okuqulethe uhlalutyo lweemetagenomics ezahlukeneyo ezifunekayo ngokuqhelekileyo kubandakanywa ukusetyenzwa kwedatha, izifundo zenqanaba leentlobo, izifundo ze-gene function-level studies, i-metagenome binning, njl. Ukongeza, izixhobo zokumbiwa kwedatha ezilungiselelwe ziyafumaneka ekuhlalutyweni okusemgangathweni komsebenzi, kubandakanywa nofuzo kunye nombuzo weentlobo. , ukusetwa kweparamitha, ukuvelisa amanani omntu, njl.
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LncRNA
Ii-RNA ezinde ezingezizo iikhowudi (lncRNA) luhlobo lwemibhalo enobude obude kune-200 nt, engakwaziyo ukufaka iiprotheyini zekhowudi.Ubungqina obuninzi bucebisa ukuba uninzi lwee-lncRNAs kusenokwenzeka ukuba zisebenze.Itekhnoloji yokulandelelaniswa komgangatho ophezulu kunye nezixhobo zokuhlalutya i-bioinformatic zisixhobisa ukuba siveze ulandelelwano lwe-lncRNA kunye nokubeka ulwazi ngokufanelekileyo kwaye sikhokelele ekufumaneni ii-lncRNAs ezinemisebenzi ebalulekileyo yokulawula.I-BMKCloud inebhongo ngokubonelela abathengi bethu ngeqonga lokuhlalutya lolandelelwano lwe-lncRNA ukuphumeza uhlalutyo olukhawulezayo, oluthembekileyo noluguquguqukayo lwe-lncRNA.
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I-GWAS
I-Genome-wide association study (GWAS) ijolise ekuchongeni ukuhlukahluka kofuzo (i-genotype) ehambelana neempawu ezithile (phenotype).Izifundo ze-GWA ziphanda iimpawu zofuzo ezinqamlezileyo zegenome yenani elikhulu labantu kwaye ziqikelela unxulumano lwe-genotype-phenotype ngohlalutyo lwamanani kumgangatho wabemi.Ulandelelwano lwejini yemfuza yonke lunakho ukufumana zonke iintlobo ezahlukeneyo zemfuza.Ukudibanisa kunye nedatha ye-phenotypic, i-GWAS inokucutshungulwa ukuchonga i-phenotype ehambelana ne-SNPs, ii-QTL kunye neegenes zabaviwa, ezixhasa ngamandla ukuzala kwezilwanyana / izityalo zanamhlanje.I-SLAF sisicwangciso esenziwe lula sokulandelelana kwe-genome, efumanisa iimpawu ezisasazwe ngokubanzi ze-genome, i-SNP.Ezi SNP, njengabamakishi be-molecular genetic, banokucutshungulwa kwizifundo zombutho kunye neempawu ezijoliswe kuzo.Sisicwangciso esingabizi kakhulu ekuchongeni iimpawu ezintsonkothileyo ezinxulumene nokwahluka kofuzo.
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I-Nanopore Ubude obupheleleyo be-transcriptomics
Ii-isoforms ezintsonkothileyo neziguquguqukayo kwizinto eziphilayo ziindlela ezibalulekileyo zofuzo zokulawula ukubonakaliswa kofuzo kunye nokwahluka kweeprotheyini.Ukuchongwa ngokuchanekileyo kwezakhiwo ze-transcript sisiseko sophononongo olunzulu lweendlela zokulawula i-gene expression.Iqonga lolandelelwano lweNanopore lizise ngempumelelo uphononongo lwe-transcriptomic kumgangatho we-isoform.Eli qonga lokuhlalutya lenzelwe ukuhlalutya idatha ye-RNA-Seq eveliswe kwiqonga leNanopore kwisiseko se-genome yereferensi, efezekisa uhlalutyo olunobulunga kunye nobungakanani kwinqanaba le-gene kunye nenqanaba lokubhaliweyo.
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malunga ne-RNA
I-RNA yeSetyhula (i-circRNA) luhlobo lwe-RNA engeyiyo ikhowudi, efunyenwe kutshanje ukuba idlala indima ebalulekileyo kuthungelwano olulawulayo olubandakanyekayo ekuphuhliseni, ukuchasana nokusingqongileyo, njl njl. Ukwahlula kwiimolekyuli ze-RNA ezinomgca, umzekelo, i-mRNA, i-lncRNA, i-3 kunye ne-5 iziphelo ze-circRNA zidityaniswe kunye zenza isakhelo sesetyhula, esibasindisa ekugayweni kwe-exonuclease kwaye zizinzile ngakumbi kune-RNA eninzi.I-CircRNA ifunyenwe inemisebenzi eyahlukeneyo yokulawula ukubonakaliswa kofuzo.I-CircRNA inokusebenza njenge-ceRNA, ebopha i-miRNA ngokhuphiswano, eyaziwa ngokuba yi-miRNA sponge.Iqonga lokuhlalutya lolandelelwano lwe-CircRNA lixhobisa ubume be-circRNA kunye nohlalutyo lwentetho, ukuqikelela okujoliswe kuko kunye nohlalutyo oludibeneyo kunye nezinye iintlobo zeemolekyuli ze-RNA.
