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I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)
I-ChIP-Seq inikezela ngeprofayili ye-genome-wide-wide ye-DNA ekujoliswe kuyo yokuguqulwa kwe-histone, izinto ezibhaliweyo, kunye nezinye iiprotheni ezinxulumene ne-DNA.Idibanisa ukukhethwa kwe-chromatin immuno-precipitation (i-ChIP) ukubuyisela i-protein-DNA complexes ethile, kunye namandla okulandelelanisa isizukulwana esilandelayo (NGS) ukulandelelana kwe-high-throughput ye-DNA efunyenweyo.Ukongeza, ngenxa yokuba iiprotheyini ze-DNA zifunyenwe kwiiseli eziphilayo, iindawo zokubopha zinokuthelekiswa kwiintlobo zeeseli ezahlukeneyo kunye nezicubu, okanye phantsi kweemeko ezahlukeneyo.Izicelo zisusela kummiselo wokubhaliweyo ukuya kwiindlela zophuhliso kwiindlela zokugula nangaphaya.
Iqonga: I-Illumina NovaSeq Platform
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Ulandelelwano lweMetagenomic -NGS
I-Metagenome ibhekiselele kwingqokelela yemathiriyeli yemfuzo iyonke yoluntu oluxubeneyo lwezinto eziphilayo, ezifana ne-metagenome yokusingqongileyo, i-metagenome yabantu, njl njl.Ukulandelelana kwe-Metagenomic sisixhobo se-molecular esetyenziselwa ukuhlalutya izinto ezixubeneyo ze-genomic ezikhutshwe kwiisampuli zendalo, ezinikezela ngolwazi olucacileyo kwiintlobo zeentlobo zeentlobo kunye nobuninzi, ubume benani labantu, ubudlelwane be-phylogenetic, izakhi zofuzo ezisebenzayo kunye nenethiwekhi yokulungelelanisa kunye nezinto ezingqongileyo.
Iqonga:I-Illumina NovaSeq Platform
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Ulandelelwano lweMetagenomic-Nanopore
I-Metagenomics sisixhobo se-molecular esetyenziselwa ukuhlalutya izinto ezixubileyo ze-genomic ezikhutshwe kwiisampuli zendalo, ezinikezela ngolwazi olucacileyo kwiintlobo ezahlukeneyo zeentlobo kunye nobuninzi, isakhiwo sabantu, ubudlelwane be-phylogenetic, izakhi zofuzo ezisebenzayo kunye nenethiwekhi yokulungelelanisa kunye nezinto ezisingqongileyo, njl njl. kwizifundo zemetagenomic.Ukusebenza kwayo okugqwesileyo kubude bokufunda kuphuculwe kakhulu uhlalutyo lwemetagenomic esezantsi, ngakumbi indibano yemetagenome.Ukuthatha izibonelelo zobude bokufunda, i-Nanopore-based based metagenomic study iyakwazi ukuphumeza indibano eqhubekayo ngokuthelekisa ne-shot-gun metagenomics.Kuye kwapapashwa ukuba i-metagenomics esekwe kwi-Nanopore yenze ngempumelelo i-genomes yebhakteria epheleleyo kunye nevaliweyo ukusuka kwi-microbiomes (Moss, EL, et. al.I-Biotech yendalo, 2020)
Iqonga:Nanopore PromethION P48
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Ulandelelwano lwegenome bisulfite epheleleyo
I-DNA methylation kwindawo yesihlanu kwi-cytosine (5-mC) inempembelelo ebalulekileyo kwi-gene expression kunye nomsebenzi weselula.Iipateni ze-methylation ezingaqhelekanga ziye zadibaniswa neemeko ezininzi kunye nezifo, ezifana nomhlaza.I-WGBS iye yaba ngumgangatho wegolide wokufunda i-genome-wide methylation kwisisombululo sesiseko esinye.
Iqonga: I-Illumina NovaSeq Platform
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I-Assay ye-Chromatin eFikelelekayo yeTransposase kunye noSetyenziso lwe-High throughput (ATAC-seq)
I-ATAC-seq yindlela ephezulu yokulandelelanisa i-genome-wide-wide chromatin accessibility, ebaluleke kakhulu kulawulo lwe-epigenetic lwehlabathi jikelele lwe-gene expression.Iiadaptha zolandelelwano zifakwe kwimimandla yechromatin evulekileyo nge-hyperactive Tn5 transposase.Emva kokwandiswa kwePCR, ithala leencwadi lolandelelwano liyakhiwa.Yonke imimandla ye-chromatin evulekileyo inokufumaneka phantsi kwemeko ethile yexesha lesithuba, ingaphelelanga kuphela kwiindawo ezibophelelayo ze-transcription factor, okanye ummandla othile we-histone modified.
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16S/18S/ITS Amplicon Sequencing-PacBio
I-subunit ekwi-16S kunye ne-18S rRNA equlethe yomibini imimandla egcinwe kakhulu kunye ne-hyper-variable printing yeminwe egqibeleleyo yemolekyuli yokuchongwa kwe-prokaryotic kunye ne-eukaryotic organisms.Ukuthatha ithuba lolandelelwano, ezi amplicon zinokujoliswa ngokusekwe kumacandelo agciniweyo kwaye imimandla eguquguqukayo ephezulu inokubonakaliswa ngokupheleleyo ukuchongwa kwemicrobial enegalelo kwizifundo ezibandakanya uhlalutyo lwentlupheko yemicrobial, taxonomy, phylogeny, njl. njl. ) ulandelelwano lweqonga lePacBio lwenza ukuba kufumaneke ufundo olude oluchaneke kakhulu, olunokugubungela i-amplicon yobude obupheleleyo (malunga ne-1.5 KB).Imbono ebanzi yendawo yemfuza yasiphucula kakhulu isisombululo kwingcaciso yeentlobo kwiibhaktiriya okanye kuluntu lomngundo.
Iqonga:I-PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
I-16S/18S/ITS i-amplicon sequencing ijolise ekuvezeni i-phylogeny, taxonomy, kunye nobuninzi beentlobo kuluntu lwe-microbial ngokuphanda iimveliso ze-PCR zeempawu zofuzo zokugcina izindlu eziqulethe zombini iindawo ezixoxwa kakhulu kunye ne-hypervariable.Ukwaziswa kwezi minwe zigqibeleleyo zemolekyuli ngu-Woeses et al, (1977) ixhobisa iprofayili ye-microbiome ezimeleyo.Ulandelelwano lwe-16S (iibhaktheriya), i-18S (umngundo) kunye ne-Internal transcribed spacer(ITS, ukungunda) ivumela ukuchongwa kweentlobo ezininzi kunye neentlobo ezinqabileyo nezingaziwa.Le teknoloji iye yaba sisixhobo esisetyenziswa ngokubanzi kunye nesiphambili ekuchongeni ukubunjwa kwe-microbial eyahlukileyo kwiindawo ezahlukeneyo, ezinjengomlomo womntu, amathumbu, ilindle, njl.
Iqonga:I-Illumina NovaSeq Platform
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IBacterial kunye neFungal Whole Genome Re-Sequencing
Ukulandelelana kwakhona kwejenome yebhaktiriya kunye nomngundo sisixhobo esibalulekileyo sokugqiba iigenomes zebhaktiriya eyaziwayo kunye nomngundo, kunye nokuthelekisa iigenomes ezininzi okanye ukwenza imephu yejenomi yezinto ezitsha.Kubaluleke kakhulu ukulandelelanisa iigenomes zonke zebhaktiriya kunye nomngundo ukuze kuveliswe iigenome zesalathiso ezichanekileyo, ukuchongwa kwe-microbial kunye nolunye uphononongo lothelekiso lwe-genome.
Iqonga: Iqonga le-Illumina NovaSeq
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PacBio-Full-length 16S/18S/ITS Amplicon Sequencing
Iqonga le-Amplicon (16S / 18S / ITS) liphuhliswa kunye neminyaka yamava kuhlalutyo lweprojekthi ye-microbial diversity, equlethe uhlalutyo olusisiseko olusemgangathweni kunye nohlalutyo lomntu siqu: uhlalutyo olusisiseko lubandakanya umxholo wohlalutyo oluqhelekileyo lophando lwangoku lwe-microbial, umxholo wokuhlalutya ucebile kwaye ubanzi, kunye neziphumo zohlalutyo zinikezelwe ngohlobo lweengxelo zeprojekthi;Umxholo wohlalutyo lobuqu uhlukile.Iisampulu zinokukhethwa kwaye iiparameters zinokumiselwa ngokuguquguqukayo ngokwengxelo yohlalutyo olusisiseko kunye nenjongo yophando, ukufezekisa iimfuno zomntu.Inkqubo yokusebenza yeWindows, ilula kwaye iyakhawuleza.
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I-PacBio-ubude obugcweleyo be-Transcriptome (Non-Reference)
Ukuthatha i-Pacific Biosciences (i-PacBio) ye-Isoform yokulandelelanisa idatha njengegalelo, le App iyakwazi ukuchonga ulandelelwano olupheleleyo lwe-transcript (ngaphandle kwendibano).Ngokwenza imephu yolandelelwano lobude obupheleleyo ngokuchasene ne-genome yereferensi, imibhalo inokuthi iphuculwe ngejene eyaziwayo, imibhalo ebhaliweyo, imimandla yekhowudi, njl.njl. Kule meko, ukuchongwa okuchaneke ngakumbi kwezakhiwo ze-mRNA, njengokunye ukudityaniswa, njl njl, kunokufezekiswa.Uhlalutyo oludityanisiweyo kunye nedatha ye-NGS yolandelelwano lwe-transcriptome yenza ukuba inkcazo ebanzi ngakumbi kunye nobungakanani obuchanekileyo ekubonakalisweni kwinqanaba le-transcript, elizuza kakhulu kwintetho ephantsi yolwahlulo kunye nohlalutyo olusebenzayo.
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Umekelo oluNcitshisiweyo lweBisulfite Sequencing (RRBS)
Uphando lwe-DNA methylation luhlala luyisihloko esishushu kuphando lwezifo, kwaye luhambelana ngokusondeleyo ne-gene expression kunye ne-pheno-typic traits.I-RRBS yindlela echanekileyo, esebenzayo kunye neyoqoqosho yophando lwe-DNA methylation.Ukutyetyiswa komgqugquzeli kunye nemimandla yesiqithi se-CpG nge-enzymatic cleavage (Msp I), idityaniswe nokulandelelana kwe-Bisulfite, ibonelela ngesisombululo esiphezulu se-DNA methylation.
Iqonga: I-Illumina NovaSeq Platform
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Ulandelelwano lweProkaryotic mRNA
Ulandelelwano lwe-mRNA luxhobisa iprofayili ebanzi yazo zonke iikopi ze-mRNA ngaphakathi kweeseli phantsi kweemeko ezithile.Le teknoloji ye-cutting-edge isebenza njengesixhobo esinamandla, ityhila iiprofayili zegenesis ezintsonkothileyo, ulwakhiwo lwemfuza, kunye neendlela zemolekyuli ezinxulumene neenkqubo ezahlukeneyo zebhayoloji.Yamkelwa ngokubanzi kuphando olusisiseko, uxilongo lweklinikhi, kunye nophuhliso lwamachiza, ulandelelwano lwe-mRNA lubonelela ngokuqonda kubuntsonkotha bokutshintshatshintsha kweeseli kunye nolawulo lwemfuzo.Inkqubo yethu yesampulu yeprokaryotic mRNA ilungiselelwe i-prokaryotic transcriptomes, ebandakanya ukuncipha kwe-rRNA kunye nokulungiswa kwethala leencwadi.
Iqonga: Illumina NovaSeq X
