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Iindaba

UKUVELA KWEGENOME

PNAS

Imvelaphi yendalo kunye nembali yokwenziwa kwasekhaya kwentlanzi yegolide (iCarassius auratus)

PacBio |Ilumina |Imephu yeBionano Genome |INdibano ye-Hi-C Genome |Imephu yemfuzo |GWAS |I-RNA-Seq

Iimbalasane

I-1.Goldfish genome yahlaziywa nge-high-quality assembly version, i-anchoring 95.75% ye-contigs kwi-50 pseudochromosomes (Scaffold N50 = 31.84 Mb).Ii-subgenomes ezimbini zaqhawuka.

2.Imimandla yeGenomic yokutshayela okukhethiweyo ngexesha lokufuywa ekhaya ichongiwe ukusuka kulandelelwano lwedatha yabantu abangama-201, kutyhilwa ngaphezulu kwama-390 emfuzo ekusenokwenzeka ukuba yayanyaniswa neempawu zasekhaya.

I-3.GWAS kwi-dorsal fin kwi-goldfish efuywayo iveze ama-378 emfuza enokuthi yayanyaniswa.Intatheli ye-tyrosine-protein kinase ichongiwe njenge-gene causal gene ehambelana nokungafihli

Imvelaphi

I-Goldfish (i-Carassius auratus) yenye yezona ntlanzi zibalulekileyo ezifuywayo, ezazifuywa kwi-crucian carp kwi-China yakudala.Baye bahlonitshwa nguCharles Darwin njengo "Sidlula kwibala eliphantse lingenasiphelo, sidibana nolona hlengahlengiso lumangalisayo lolwakhiwo".Iimpawu ezahlukeneyo kakhulu kunye nembali ende yokufuywa kwasekhaya kunye nokuzala kwenza i-goldfish ibe yinkqubo yemfuzo egqwesileyo yefiziyoloji yeentlanzi kunye nendaleko.

Impumelelo

I-Goldfish genome

Juhlalutyo lwe-oint lwe-PacBio kunye ne-Illumina i-pair-end sequencing data ivelisa i-1.657 G yendibano yedrafti yokuqala (Contig N50 = 474 Kb).Imephu ye-Bonano optical yenziwe kwaye yalungisa indibano ibe yi-1.73 Gb ngobukhulu (Ubungakanani obuqikelelweyo be-genome: 1.8 Gb).Indibano esekwe kwi-Hi-C iphucule ngakumbi i-scaffold i-N50 ukusuka kwi-606 Kb ukuya kwi-31.84 Mb kwaye yazuza i-95.75% (1.65 Gb) eqhelanisiweyo kunye nereyithi ye-anchoring.I-genome ibandakanya i-56,251 yohlobo lwe-coding kunye ne-10,098 ye-non-coding transcript ende.Ngaphezu koko, imimandla engama-38 enokubakho ye-centromeric yaqikelelwa kwiichromosomes ezingama-50.

iindaba eziphambili-pnas-goldfish-fig1

Umzobo.1 Intlanzi yeGolide yeGenome

TImbi kakhulu iiseti ezicacileyo zeesubgenomes zachongwa kwiichromosome ze-goldfish ezingama-50 eziphuma kwisiganeko sakudala sokuxubana.Iseti yeechromosomes ezinomlinganiselo ophezulu wokufundwa olungelelaniswe phakathi kwe-goldfish ne-Barbinae zichazwe njenge-subgenome A (ChrA01~A25), okt i-subgenome eqhelekileyo ku-Barbinae, kunye nentsalela njenge-subgenome B (ChrB01~B25).

Ukutshayela kwasekhaya kunye nokukhethiweyo

AItotali ye-16 yohlobo lwecrucian carps kunye ne-185 emele i-goldfish emele ezahlukeneyo ziye zalandelelwa ngomndilili wolandelelwano ubunzulu obumalunga ne-12.5X, ivelisa i-4.3 terabases zedatha.Ukuhlaziywa kwe-Phylogenetic kunye nohlalutyo lwe-PCA luqinisekisile ubudlelwane obusondeleyo phakathi kwe-goldfish eqhelekileyo kunye ne-crucian carp kunezinye i-goldfish, eziye zahlulahlula zibe yimizila emibini.

iindaba eziphambili-pnas-goldfish-fig2-1-1024x287

LUhlalutyo lwe-D lokubola kwi-subpopulations engaphezulu kwezine luxhase ubukho be-bottleneck yemfuza yabemi ngexesha lokufuywa kwasekhaya kunye nokukhetha okunamandla okwenziweyo kwi-goldfish.Ukonyuka kweyantlukwano yemfuza (π) ukusuka kwicrucian carp ukuya kwintlanzi eqhelekileyo yegolide ukuya kwi-Wen goldfish kunye ne-Egg goldfish ibonise uqokelelo olubalulekileyo lweeyantlukwano zemfuza ngexesha lokufuzwa kwazo.Imimandla engama-50 ekhethiweyo yokutshayela i-genomic equka i-25.2 Mb kunye nama-946 emfuza achongiwe kwiinkcukacha zabameli (33 goldfish kunye ne-16 crucian crap).Ukwandisa uhlalutyo kubantu abangama-201, iijini ezingama-393 zibonise imimandla yokutshayela okukhethiweyo.Ezi ntlobo zofuzo zifunyenwe zineyantlukwano ephantsi, ekunokwenzeka ukuba ibe negalelo kwiiphenotypes ezinxulumene neempawu eziphambili zokufuya kwintlanzi yegolide.

iindaba eziphambili-pnas-goldfish-fig3-1024x451

Umzobo.3 Uhlalutyo olunxulumene ne-Genome-wide domestication

I-GWAS kwintlanzi yegolide yasekhaya

Di-orsal fin luphawu oluphambili olwahlula i-Wen goldfish kwi-Egg goldfish.I-GWAS ye-dorsal fin kwi-96 Wen goldfish kunye ne-87 Egg goldfish ibonise ama-378 emfuzo esele isasazeke kwiichromosomes ezili-13 kwaye usasazo olungalinganiyo lwezi mfuza phakathi kwe-subgenomes luye lwabonwa.Uhlalutyo olusebenzayo kula majene abaviwa abonisa iinkqubo zebhayoloji ezibandakanya "i-Cell surface receptor signaling", "Transmembrane transportation", "Skeletal system development", njl.

iindaba eziphambili-pnas-goldfish-fig4-1024x353

Umfanekiso we-4 GWAS we-dorsal fin kwi-goldfish yasekhaya

In I-GWAS yeempawu ezinxulumene nesikali esicacileyo, incopho yombutho omnye owomeleleyo yafunyanwa.I-gene encoding i-tyrosine-protein kinase receptor ichongiwe kwenye yemimandla yomviwa.

iindaba eziphambili-pnas-goldfish-fig5-1024x271

Umzobo we-5 GWAS weempawu ezinxulumene nesikali esicacileyo

Isalathiso

Chen D et al.Imvelaphi yendalo kunye nembali yokufuya intlanzi yegolide (iCarassius auratus).I-PNAS (2020)

Iindaba  ijolise ekwabelaneni ngamatyala aphumeleleyo amva nje kunye ne-Biomarker Technologies, ukubamba impumelelo yesayensi enoveli kunye nobuchule obubalaseleyo obusetyenzisiweyo ngexesha lokufunda.


Ixesha lokuposa: Jan-04-2022

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