UKUVELA KWEGENOME
IGenome De novo
INdibano|Ukumiselwa kwezesondo Yonke imisebenzi yolandelelwano kunye nenxalenye yeenkonzo ze-bioinformatic zanikezelwa yi-Biomarker Techonologies.
Abstract
“I-phenotype ye-conic phenotype yeedragons zaselwandle ibandakanya i-appendages efana namagqabi, umlomo we-tubular ongenamazinyo, kunye nokukhulelwa kwendoda okubandakanya ukufukanyelwa kwamaqanda aqhanyiweyo "kwi-brood patch" evulekileyo.We de novo–ilandelelanisa iigenomes zenkunzi nemazi zeseadragon eqhelekileyo (Phyllopteryx taeniolatus) kunye neentlobo zayo ezisondeleleneyo, i alligator pipefish (Syngnathoides biaculeatus).Iiprofayili ezikhutshelweyo ezivela kwinto entsha, izihlomelo ezifana neqabunga, zibonisa ukuba iseti yemfuza edla ngokubandakanyeka kuphuhliso lokugqibela iye yanyulwa kunye nokutyebisa imibhalo ekhutshelweyo yokulungiswa kwezicubu ezinokubakho kunye nofuzo lokhuselo lomzimba.I-zebrafish mutants ye-scpp5, elahlekileyo kuzo zonke ii-sygnathids, zifunyenwe zingenalo okanye zinezinyo eziphazamisayo ze-pharyngeal, ezixhasa i-hypothesis yokuba ukulahlekelwa kwe-scpp5 kuye kwaba negalelo ekulahlekeni kwamazinyo kwi-sygnathids.Indawo ekujongwa kuyo isini-emisela i-locus efaka ikhowudi yofuzo ye-amhr2y yendoda ekwabelwana ngayo yi-common seadragon kunye ne-alligator pipefish ichongiwe. ”
Impahla nenkqubo:
Izinto eziphathekayo
Finyoka yethu eqhelekileyo (P. taeniolatus) kunye nengwenya ezimbini (S. biaculeatus) imizekelo.Inyoka yaselwandle ezimbini eziqhelekileyo (indoda enye kunye nemazi enye) kunye ne-alligator pipefish (indoda enye kunye nebhinqa enye) abantu basetyenziselwa ulandelelwano lwe-genome epheleleyo, kunye nabanye abantu ababini abaqhelekileyo be-seadragon abaqhelekileyo basetyenziselwa ukulandelelana kwe-genome.
Isicwangciso seGenome Sequencing
2Ithala leencwadi le-70/350 bp (Hiseq 2500) + Pacbio 20Kb (Sequel) okanye iNanopore 30 Kb (MinION)+ Hi-C (NovaSeq 6000).ulandelelwano, kunye nabanye abantu ababini abaqhelekileyo be-seadragon ngabanye basetyenziselwa ukulandelelana kwegenome.
Ulandelelwano lwe-Transcriptome
Fokanye i-seadragon eqhelekileyo, ingqondo, iliso, i-gill, isibindi, intliziyo, izintso, i-mesentery, amathumbu, imisipha, i-fin, isikhumba, i-appendages efana neqabunga, i-testis, kunye ne-ovary.
Isicwangciso sendibano yeGenome de novo
Ci-anu (Ulungiso) + WTDBG2 (Indibano) + Pilon(Polish) + Lachesis (Hi-C).
Iziphumo eziphambili
Umzobo 1. Iimpawu eziphambili ze-common seadragon (P. taeniolatus) kunye ne-alligator pipefish (S. biaculeatus) kunye neendawo zabo ze-phylogenetic.
Umzobo 2. I-morphology kunye nemfuzo ye-appendages efana neqabunga kwi-common seadragon (P. taeniolatus).
Umzobo 3. Ufuzo olumisela isondo olubekayo kwi-common seadragon (P. taeniolatus) kunye ne-alligator pipefish (S. biaculeatus).
Umzobo 4. I-Pharyngeal tooth phenotypes kwi-zebrafish scpp5 i-homozygous mutants.
Ixesha lokuposa: Jan-08-2022