I-GENOME EVOLUTION, I-PANGENOME
Yintoni iPan-genome?
Ubungqina obuqokelelweyo bubonisa ukuba umahluko phakathi kweentlobo ezahlukeneyo zohlobo oluthile unokuba mkhulu.I-genome enye ikude ngokwaneleyo ukufumana umfanekiso opheleleyo wolwazi lwemfuza yohlobo olulodwa.Injongo yesifundo se-Pan-genome kukufumana igrafu ebanzi ye-genomic yohlobo kunye nokuchonga ubudlelwane phakathi kweempawu kunye neekhowudi zofuzo ngokuqhuba indibano ye-genome de novo yeentlobo ezininzi, ezivumela ukumbiwa kwemigodi enzulu kunye nebanzi yeenguqu.
Iindlela zePan-genome Study
Umzobo 1 Iindlela zamaphepha ophando apapashiweyo ePan-genome.
Qaphela: Umzobo ubonisa isiphumo sokuthatha "pan-genome" njengegama eliphambili lokukhangela izihloko zamanqaku apapashwe kwiijenali zeNdalo, iiSeli kunye nezeNzululwazi.
a.Ufundo olusuka kwiisampulu ezininzi lulungelelaniswa kwireferensi kwaye ezo zingalungelelanisiweyo zidityaniswe kwiinoveli zecontigs.Ngokudibanisa ezi noveli contigs kulandelelwano lwereferensi yokuqala, ireferensi ye-pangenome inokwakhiwa.Imimandla enokusebenziseka imiselwe ngokusekelwe kwimephu yonke efundwayo emva kwipangenome.
b.Indibano ye-De novo yeegenomes zofikelelo oluninzi ivumela iindlela ezipheleleyo zolungelelwaniso lwe-genome ukuchonga imimandla ye-genomic enokuchithwa.
c.Igrafu ye-pan-genome inokwakhiwa ngolungelelwaniso lwegenome epheleleyo okanye ngedibano yegrafu ye-de novo, egcina ngokufanelekileyo ulwazi olwahlukileyo lwemimandla enokuchithwa njengeendlela ezizodwa kwigrafu.
Uyenza njani iPan-genome?
Umzobo 2 Ukuthelekiswa kweendlela ze-pan-genome1
a.Ufundo olusuka kwiisampulu ezininzi lulungelelaniswa kwireferensi kwaye ezo zingalungelelanisiweyo zidityaniswe kwiinoveli zecontigs.Ngokudibanisa ezi noveli contigs kulandelelwano lwereferensi yokuqala, ireferensi ye-pangenome inokwakhiwa.Imimandla enokusebenziseka imiselwe ngokusekelwe kwimephu yonke efundwayo emva kwipangenome.
b.Indibano ye-De novo yeegenomes zofikelelo oluninzi ivumela iindlela ezipheleleyo zolungelelwaniso lwe-genome ukuchonga imimandla ye-genomic enokuchithwa.
c.Igrafu ye-pan-genome inokwakhiwa ngolungelelwaniso lwegenome epheleleyo okanye ngedibano yegrafu ye-de novo, egcina ngokufanelekileyo ulwazi olwahlukileyo lwemimandla enokuchithwa njengeendlela ezizodwa kwigrafu.
IPan-genomes esanda kupapashwa
● Rape pan-genomes2
● I-tomato pan-genomes 3
● I-Rice Pan -genome4
● Ujongilanga iPan-genome5
● Iimbotyi zesoya iPan-genome 6
● Irayisi iPan-genome7
● Irhasi yePan-genome8
● Ingqolowa iPan-genome9
● Amazimba ePan-genome10
● I-Phytoplankton Pan-genome11
Isalathiso
1. I-Bayer PE, i-Golicz AA, i-Scheben A, i-Batley J, i-Edward D. I-pan-genomes yezityalo yireferensi entsha.Izityalo zeNat.2020;6(8):914-920.doi:10.1038/s41477-020-0733-0
2. Ingoma uJM, uGuan Z, uHu J, nabanye.Iigenomes ezisibhozo ezikumgangatho ophezulu zibonisa uyilo lwe-pan-genome kunye ne-ecotype umahluko we-Brassica napus.Izityalo zeNat.2020;6(1):34-45.doi:10.1038/s41477-019-0577-7
3. Gao L, Gonda I, Sun H, et al.I-tomato pan-genome ityhila imfuza entsha kunye nencasa enqabileyo elawula iziqhamo.UNat Genet.2019;51(6):1044-1051.doi:10.1038/s41588-019-0410-2
4. Zhao Q, Feng Q, Lu H, et al.Uhlalutyo lwe-Pan-genome lubonisa ubungakanani benguqu ye-genomic kwirayisi elinywayo kunye nelayisi yasendle [ulungiso olupapashiweyo lubonakala kwiNat Genet.2018 Agasti; 50 (8): 1196].UNat Genet.2018;50(2):278-284.doi:10.1038/s41588-018-0041-z
5. UHübner S, uBercovich N, uTodesco M, et al.Uhlalutyo lwe-sunflower pan-genome lubonisa ukuba i-hybridization iguqule umxholo we-gene kunye nokumelana nezifo.Izityalo zeNat.2019;5(1):54-62.doi:10.1038/s41477-018-0329-0
6. ULiu Y, uDu H, uLi P, kunye no-al.I-Pan-Genome yeembotyi zesoya zasendle kunye nezilinyiweyo.Iseli.2020;182(1):162-176.e13.doi:10.1016/j.cell.2020.05.023
7. Qin P, Lu H, Du H, et al.Uhlalutyo lwe-pan-genome lwe-33 yokufikelela kwirayisi eyohlukeneyo ngokwemfuza ityhila ukuhluka okufihliweyo kwe-genomic [epapashwe kwi-intanethi ngaphambi kokuprintwa, ngo-2021 ngoMeyi 25].Iseli.2021;S0092-8674(21)00581-X.doi:10.1016/j.cell.2021.04.046
8. Jayakodi M, Padmarasu S, Haberer G, et al.I-pan-genome yebhali ityhila ilifa elifihliweyo lokuzalana kwenguqu.Indalo.2020;588(7837):284-289.doi:10.1038/s41586-020-2947-8
9. Walkowiak S, Gao L, Monat C, et al.Iigenomes ezininzi zengqolowa zibonisa ukwahluka kwehlabathi ekuzaleni kwale mihla.Indalo.2020;588(7837):277-283.doi:10.1038/s41586-020-2961-x
10. Tao Y, Luo H, Xu J, et al.Ukwahluka okubanzi ngaphakathi kwe-pan-genome yamazimba alinyiweyo kunye namazimba asendle [epapashwe kwi-intanethi ngaphambi kokushicilelwa, ngo-2021 ngoMeyi 20].Izityalo zeNat.2021;10.1038 / s41477-021-00925-x.doi:10.1038/s41477-021-00925-x
11. Umlandeli X, Qiu H, Han W, et al.I-Phytoplankton pangenome ibonisa ukudluliselwa kwemfuza okuthe tye kweprokaryotic yemisebenzi eyahlukeneyo.Sci Adv.2020;6(18):eaba0111.Ipapashwe 2020 Epreli 29. doi:10.1126/sciadv.aba0111
Ixesha lokuposa: Jan-04-2022