Kwi-2021, i-BMKGENE ibone i-31 ye-De novo yophando lwe-genome epapashwe ngempumelelo kwiijenali ezinempembelelo ephezulu kunye nezinto ezichaphazelayo ezipheleleyo ngaphezu kwe-320. I-15 yamanqaku abhalwe ngokudibeneyo 4 kubo abhalwa ngokubambisana njengababhali bokuqala yi-BMKGENE.
Kanye emva kokuqala konyaka ka-2022, sele kukho amanqaku ophando amabini apapashwe kwijenali ethi “Natural Genetics” kunye “neSityalo seMolekyuli” ngokulandelelanayo.Zizo, "Ii-haplotypes ezimbini ezihlukeneyo ezivela kwi-heterozygous lychee genome ephezulu zicebisa iziganeko ezizimeleyo zokufuywa kwasekhaya kwiicultivars zakwangoko kunye nezivuthwa kade" (uphando lweLychee genome, oluqhutywa yiKholeji yeHorticulture, iYunivesiti yezoLimo yaseMzantsi China, kunye nabalingane bezesayensi, epapashwe kwi-Natural Genetics. ), kunye “nolandelelwano lweGenome lweentlobo ezintlanu zeSitopsis zeAegilops kunye nemvelaphi yepolyploid wheat B-subgenome” (Five Sitopsiss species genome, eqhutywa liqela lophando likaNjingalwazi Bao Liu weYunivesithi yaseNortheast Normal.).Kwakhona siza kuphonononga la manqaku mabini kwaye sabelane nabafundi bethu.
Ngoku, makhe sijonge kumanqaku ophando abalaseleyo apapashwe ngo-2021 abhalelwe yi-BMK kunye nezixhobo zethu ezisebenzisanayo.
IGenome yeZityalo-Ukuphumelela kwiintlobo ezininzi.
1.Indibano yegenome ekumgangatho ophezulu iqaqambisa iimpawu ze-rye genomic kunye nofuzo olubalulekileyo ngokwezolimo
IZiko eliDityanisiweyo: IYunivesithi yezoLimo yaseHenan
Ijenali: Imfuzo yendalo
Umba weMpembelelo: 38.31
Kule projekthi, i-genome ye-Weining rye, udidi lwe-rye lwaseTshayina oluphezulu, lulandelelaniswe.I-contigs edibeneyo (i-7.74 Gb) yenza i-98.47% yobungakanani obuqikelelweyo be-genome (7.86 Gb), kunye ne-93.67% ye-contigs (7.25 Gb) eyabelwe iichromosomes ezisixhenxe.Izinto eziphindaphindayo zenze i-90.31% yegenome edityanisiweyo.Ucazululo olongezelelweyo lwendibano ye-Weining lukhanyise ukukhanya okutsha kuphindaphindo lwemfuza ebanzi kunye nefuthe lazo kwi-starch biosynthesis genes, umbutho womzimba we-prolamin loci entsonkothileyo, iimpawu zokubonakaliswa kofuzo olusisiseko sesihloko sakwangoko kunye nemimandla yechromosomal enxulumene nokufuywa ekhaya kunye neloci kwirye.Olu landelelwano lwe-genome luthembisa ukukhawulezisa izifundo ze-genomic kunye nokuzalanisa kwi-rye kunye nezityalo ze-cereal ezinxulumene nazo.
I-2.I-Rose ngaphandle kwe-prickle: i-genomic insights edibene nokulungelelaniswa komswakama
IZiko eliDityanisiweyo: iKunming Institute of botany, Chinese Academy of Sciences
Ijenali: Uphononongo lweNzululwazi yeSizwe
Impembelelo yempembelelo: 17.273
Kule projekthi, iisampulu ze-'Basye's Thornless' (BT, umhlanganiselwa ongavuthwanga we-Rosa wichuraiana), 'Old Blush' (OB, umseki we-genotype kwi-rose domestication), imihlanganiselwa yabo ye-F1 kunye ne-BCF1 zaqokelelwa.Kwaye kwaveliswa udidi oluphezulu lwereferensi yegenome ukuchonga izinto zofuzo ezinxulumene nophuhliso lwe-stem prickle.Ubungakanani begenome bumalunga ne-530.6 Mb.Ukuqinisekisa umgangatho we-genome edibeneyo, uhlalutyo olufana nothelekiso lwemephu yemfuza, i-BUSCO, i-NGS ifunda ukuphinda iphinde ihlangane, ithelekisa ne-OB haplotype, ulandelelwano lolawulo lwempazamo yesiseko kunye ne-genome-wide LTR Assembly Index value check (LAI=20.03) yenziwe.Olu phando lubonisa ipateni entsonkothileyo yelifa kunye nenkqubo yolawulo ye-stem prickles kwaye isinike isiseko kunye nemithombo yenoveli yokufunda ibhayoloji yerose kunye nomgodi wokumakisha iimolekyuli ezinxulumene neempawu ezibalulekileyo.
I-3.I-Whole-Genome Sequence ye-Synthesized Allopolyploids kwi-Cucumis ityhila i-Insights kwi-Genome Evolution ye-Allopolyploidization
Indawo eDityanisiweyo: IYunivesithi yezoLimo yaseNanjing
Ijenali: Advanced Science
Impembelelo yempembelelo: 16.801
Olu phononongo luchaze i-genome yomgangatho ophezulu we-allotetraploid yokwenziwa efunyenwe kusetyenziswa i-interspecific hybridization phakathi kwe-cucumber (C. sativus, 2n = 14) kunye neentlobo zayo zezilwanyana zasendle (C. hystrix, 2n = 24) kunye nokuphindaphinda kwechromosome elandelayo, engowokuqala. iallopolyploid eyenziwe ngolandelelwano ngokupheleleyo.Indibano ye-genome isebenzise ukuhamba komsebenzi kwe-"PacBio+BioNano+Hi-C+Illumina" ngokulandelelana, kubangele ubungakanani be-genome ye-530.8Mb kunye ne-contig N50 = 6.5Mb.Ufundo lwabelwa kwiipseudochromosomes ezili-19 kunye nee-subgenomes.Iziphumo zibonise ukuba ukuxubana, endaweni yokuphindaphinda i-genome, kubangela uninzi lweenguqu ze-genomic kuzo zombini i-nuclear kunye ne-cp genomes.Icebise ukuba i-heterozygosity esisigxina inika i-C. × hytivus ngokunyuka koxinzelelo.Iziphumo zibonelela ngemibono emitsha kwi-plant polyploidy evolution kwaye zibonelela ngesicwangciso esilindelekileyo sokuzala kwizityalo ezizayo.
I-4.I-Genome yokuthelekisa ihlalutya i-Highlight Transposon Mediated Genome Ukwandiswa kunye ne-Evolutionary Architecture ye-3D Genomic Folding kwiCotton.
IZiko eliDityanisiweyo: IYunivesithi yezoLimo yaseHuazhong
Ijenali: Ibhayoloji yeMolekyuli kunye nokuZiphendukela kwemvelo
Impembelelo yempembelelo: 16.242
Le projekthi isebenzise iNanopore Sequencing ukudibanisa i-genome yeentlobo ezintathu zekotoni ezizezi: Gossypium rotundifolium (K2, ubukhulu begenome = 2.44Gb, contigN50 = 5.33Mb), G. arboreum (A2, ubukhulu begenome = 1.62Gb, 1. G. raimondii (D5, ubukhulu begenome = 0.75Gb, contigN50 = 17.04 Gb).Ngaphezulu kwe-99% yazo zontathu iigenomes zadityaniswa nge-Hi-C.Iziphumo zohlalutyo lweBUSCO ziyi-92.5%, 93.9% kunye ne-95.4% ngokulandelanayo.Onke la manani abonise ukuba iigenomes ezintathu zendibano zireferensi-ibakala.Uhlalutyo lwe-genome oluthelekisayo lubhalwe iinkcukacha ze-TE yokukhulisa ngokuthe ngqo kumgca onegalelo kumahluko omkhulu wesayizi ye-genome.Olu pho nonongo lunika ukukhanya kwindima yokwandiswa kwegenome ye-transposon-mediated kwi-evolution ye-high-order chromatin structure kwizityalo.
5. Indibano yomlinganiselo weChromosome kunye nohlalutyo lwesityalo se-biomass Miscanthus lutarioriparius genome
IZiko eliDityanisiweyo: Iziko le-CAS lokuGqwesa kwiiSayensi zeZityalo zemolekyuli
Ijenali: uNxibelelwano lweNdalo
Impembelelo yempembelelo: 14.912
Le projekthi ibike indibano yechromosome-scale ye-Miscanthus lutarioriparius genome ngokudibanisa iOxford Nanopore yokulandelelana kunye nobuchwepheshe be-Hi-C.Indibano ye-2.07Gb ihlanganisa i-96.64% ye-genome, kunye ne-contig N50 ye-1.71 Mb.Malunga ne-94.30% yolandelelwano lulonke lufakwe kwiipseudochromosomes ezili-19.Ngokuthelekisa ukulandelelana kwe-BAC, ukuhlolwa kwe-LAI, ukuvavanywa kwe-BUSCO, ukudibanisa kwakhona kunye nedatha ye-NGS, ukudibanisa kwakhona idatha ye-transcriptome, i-genome yavavanywa njengomgangatho ophezulu kunye nokuqhubeka.Imvelaphi ye-Allotetraploid ye-M. lutarioriparius iqinisekiswa ngokusebenzisa ukuphindaphinda kwesathelayithi ye-centromeric.I-Tandem ephindwe kabini yofuzo ye-M. lutarioriparius isebenzayo ityetyiswe kungekuphela nje ngokwemiqathango enxulumene nokusabela koxinzelelo, kodwa i-biosynthesis yodonga lweseli.Iimpinda zeGene mhlawumbi zidlala indima kwiC4 photosynthesis kwaye zinegalelo kwiMiscanthus C4 photosynthesis kubushushu obuphantsi.Uphando lunike ireferensi ebalulekileyo yokufunda ngezityalo ezihlala ixesha elide.
6.Indibano ye-chromosome ye-Camptotheca acuminata genome ibonelela ngokuqonda kwimvelaphi yendalo ye-camptothecin biosynthesis.
Isibonelelo esiDityanisiweyo: IYunivesithi yaseSichuan
Ijenali: uNxibelelwano lweNdalo
Impembelelo yempembelelo: 14.912
Le projekthi ibike umgangatho ophezulu, i-chromosome-level C. acuminata genome assembly, kunye nobukhulu be-genome ye-414.95Mb kunye ne-contingN50 1.47Mb.Siye safumanisa ukuba i-C. acuminata ifumana ukuphinda-phindwa kwegenome ezimeleyo kunye nentaphane yemfuza ephuma kuyo inxulumene necamptothecin biosynthesis.Ukwahlukana okusebenzayo kofuzo lwe-LAMT kunye nokuziphendukela kwemvelo kwezinto ezimbini ze-SLAS, ngoko ke, zombini zibe negalelo elikhulu kwi-camptothecin biosynthesis kwi-C. acuminata.Iziphumo zagxininisa ukubaluleka komgangatho ophezulu wendibano ye-genome ekuchongeni utshintsho lwezofuzo kwimvelaphi ye-evolution ye-metabolite yesibini.
I-7.I-allele-defined genome ibonisa ukuhlukana kwe-biallelic ngexesha lokuvela kwe-cassava
IZiko eliDityanisiweyo: iAkhademi yaseTshayina yeeNzululwazi zezoLimo zeTropiki
Ijenali: Isityalo seMolekyuli
Umba weMpembelelo: 13.162
Le projekthi ihlanganise ijenome yesalathiso yecassava ene-contigN50 1.1Mb isebenzisa iqonga le-Pacific Biosciences (PacBio) lokulandelelana.Emva kovavanyo yi-BUSCO, isalathisi se-LAI, kunye nemephu yemfuza yoxinaniso oluphezulu, i-genome edibeneyo iqinisekiswa njenge-refence-grade.Imimandla engasafunekiyo ichongiwe kwaye ii-contigs zamiselwa kwiipseudochromosomes ezili-18 kusetyenziswa amakhonkco e-Hi-C.Le genome yomgangatho ophezulu kunye ne-allele-defined reference for cassava ibalulekile ekuchongeni i-bi-alleles ehlukeneyo kwi-chromosomes e-homologous, evumela ukuphonononga ukwahlula kunye nokubonakalisa ukongamela kwe-bi-alleles kunye namandla abo okuqhuba asisiseko.Iququzelele iindlela ezintsha zokuzala kwi-cassava kunye nezinye izityalo ezinobunzulu be-heterozygous.
8. Ukuqonda kweGenomic ekukhuleni ngokukhawuleza kwe-paulownias kunye nokwakhiwa komtshayelo wamagqwirha kaPaulownia
Indawo eDityanisiweyo: IYunivesithi yezoLimo yaseHenan
Ijenali: Isityalo seMolekyuli
Umba weMpembelelo: 13.162
Le projekthi idibanise igenome yenyukliya ekumgangatho ophezulu we-Paulownia fortunei, i-511.6 Mb ngobukhulu, kunye ne-93.2% yolandelelwano oluzinze kwi-20 pseudochromosomes.Ukusebenza okuphezulu kwephotosynthetic kuphunyezwa ngokudibanisa iC3 photosynthesis kunye necrassulacean acid metabolism pathway, enokuba negalelo kumkhwa wokukhula okukhawulezayo kwemithi ye-paulownia.Ulandelelwano olongezelelweyo lwegenome ye-PaWB phytoplasma, ludityaniswe nohlalutyo olusebenzayo, lubonise ukuba i-PaWB-SAP54 esebenzayo isebenzisana ngokuthe ngqo ne-Paulownia PfSPLa, nto leyo ebangela ukuthotywa kwe-PfSPLa yindlela ehlala kuyo yonke indawo kwaye ikhokelele ekwakhekeni komtshayelo wamagqwirha.Idatha inikeze ukuqonda okubalulekileyo kwi-biology ye-paulownias kunye nendlela yokulawula ukusekwa kwe-PaWB.
I-genome yezilwanyana-Iingcinga ezinzulu zokuzivelela kweentlobo
1.I-genome yeNautilus pompilius ikhanyisa ukuvela kwamehlo kunye ne-biomineralization
IZiko eliDityanisiweyo: South China Sea Institute of Oceanology, CAS
Ijenali: I-Ecology yendalo kunye nendaleko
Impembelelo yempembelelo: 15.462
Le projekthi ibonise igenome epheleleyo yeNautilus pompilius.Ine-minimalist genome phakathi kwe-cephalopods elandelelanayo, eyi-730.58Mb ene-contigN50 = 1.1Mb.Iziphumo zovavanyo lweBUSCO ngama-91.31%.Idityaniswe ne-transcriptome, i-proteome, intsapho yemfuza kunye nohlalutyo lwe-phylogenetic, le genome inike ireferensi esisiseko kwizinto ezintsha ze-cephalopod, ezifana ne-pinhole eye kunye ne-biomineralization.Uphando lubonise ukuba umonakalo ekugqibeleleni kweqela lemfuza yeHox inokunxulumana neqokobhe elinyamalalayo leMollusks.Okubalulekileyo, ukuveliswa kwezinto ezininzi ze-genomic kubandakanya ilahleko yemfuza, ukufinyela okuzimeleyo kunye nokwandiswa kweentsapho ezithile zemfuza kunye nothungelwano lwabo lolawulo olunxulumeneyo mhlawumbi lubumba ukuvela kwe-nautilus pinhole eye.I-nautilus genome yenza isibonelelo esixabisekileyo sokwakha kwakhona iimeko zendaleko kunye nokwenziwa kwegenomic okubumba iicephalopods ezikhoyo.
I-2.Uhlalutyo lwe-Seadragon genome lubonelela ngolwazi malunga ne-phenotype kunye ne-locus yokuzimisela ngokwesondo
IZiko eliDityanisiweyo: South China Sea Institute of Oceanology, CAS
Ijenali: Inkqubela phambili kwezeNzululwazi
Umba weMpembelelo: 14.132
Le projekthi ye-novo-ilandelelanisa iigenomes zenkunzi nemazi ye-common seadragon (Phyllopteryx taeniolatus) kunye neentlobo zayo ezisondeleleneyo, i-alligator pipefish (Syngnathoides biaculeatus).Ubungakanani begenome yePhyllopteryx taeniolatus yi ~659 Mb（♂）kunye ~663 Mb(♀), kunye ne-contigN50 ye-10.0Mb kunye ne-12.1mb.ubungakanani begenome yePhyllopteryx taeniolatus yi-637 Mb（♂）kunye ~648 Mb(♀）), kunye ne-contigN50 ye-18.0Mb kunye ne-21.0Mb.Ngohlalutyo lwe-phylogenetic, i-seadragon eqhelekileyo kunye ne-alligator pipefish zi-taxon engudade wase-Syngnathinae, kwaye zahlukana malunga ne-27.3 Ma eyadlulayo.Iiprofayili ezikhutshelweyo ezivela kwinto entsha, i-appendages efana neqabunga, ibonisa ukuba isethi yejini edla ngokubandakanyeka kuphuhliso lokugqibela iye yanyulwa kunye nokutyebisa okushicilelweyo okunokubakho ukulungiswa kwezicubu kunye nofuzo lokhuselo lomzimba.Indawo ekujongwa kuyo isini-emisela i-locus efaka ikhowudi yofuzo ye-amhr2y yendoda ekwabelwana ngayo yi-common seadragon kunye ne-alligator pipefish ichongiwe.Le projekthi ibonelele ngobungqina obubalulekileyo kwizifundo zokuziphendukela kwemvelo.
Ixesha lokuposa: Sep-19-2022