IMICROBIAL
Ukuhambelana kwebhaktheriya kunye nokunyanzeliswa kwe-biochar kuphuculwe ukuthotywa kwe-tebuconazole, ukubunjwa kwe-microbiome yomhlaba kunye nokusebenza
Ubude obupheleleyo 16S amplicon ulandelelwano |PacBio HiFi |Ukwahluka kweAlfa |Ukwahluka kweBeta
Kolu phononongo, ulandelelwano olupheleleyo lwe-amplicon ye-16S yi-PacBio kunye nohlalutyo lwe-bioinformatic lunikezelwe yi-Biomarker Technologies.
Iimbalasane
I-Biochar immobilized tebuconazole-degrading bacteria I-Alcaligenes faecalis WZ-2 yaphononongwa kwi-biodegradation ye-biodegradation esebenzayo kwaye ichaphazela umhlaba ongcolileyo we-tebuconazole xa kuthelekiswa noxinzelelo olukhululekile lwe-WZ-2.
1. I-Biochar-immobilized WZ-2 ibonise ukuthotywa okusebenzayo ngakumbi kwi-tebuconazole xa kuthelekiswa ne-WZ-2 yamahhala ngokunciphisa i-tebuconazole ye-half-life in the earth from 18.7 days to 13.3 days.
2. I-Biochar-immobilized WZ-2 yakwazi ukubuyisela imisebenzi ye-enzyme ye-microbial enzyme yomhlaba, kuquka i-urease, i-dehydrogenase kunye ne-invertase, njl.
3. Iprofayili yeMicrobial kumhlaba onyangweyo we-biochar-immobilized WZ-2 ogqitywe ngokulandelelana kwe-16S yobude obupheleleyo ixhaswa ngamandla ukuba le nkqubo inokubuyisela impilo yomhlaba ngokuphucula ubume bebhaktiriya yoluntu phantsi kosulelo lwe-tebuconazole.
Amava (ayamene nolandelelwano)
Ukwahlulahlula: CK: Umhlaba wendalo;T: Umhlaba otshizwe ngetebuconazole;S: I-Tebuconazole equlethwe ngumhlaba kunye noxinzelelo lwamahhala lwe-WZ-2;BC: Tebuconazole equlethwe umhlaba kunye biochar;I-BCS: I-Tebuconazole equlethe umhlaba kunye ne-biochar immobilized WZ-2.
Isampuli: Itotali yokutsalwa kwe-DNA yomhlaba eyandisiweyo yi-16S rDNA primers
I-27F (5′-AGAGTTTGATCCTGGCTCAG-3′) kunye ne-1492R (5′-GGTTACCTTGTTACGA),repesenting ngokupheleleyo-ubude be-16S rDNA
Iqonga lokulandelelana: PacBio RS II
Isicwangciso solandelelwano: I-CCS HIFI ifundeka
Uhlalutyo lwedatha:BMKCloudI-Bioinformatic Platforming yenza i-goldfish ibe yinkqubo yemfuzo egqwesileyo yefiziyoloji yeentlanzi kunye nendaleko.
Isiphumo
Ulwakhiwo loluntu lwemicrobial yomhlaba lumiselwe yi-16S rDNA yokulandelelana.Ubutyebi be-OTU kunye ne-alpha diversity index, kubandakanywa i-Chao1, i-Ace, i-Shannon kunye ne-Simpson indices ziye zavavanywa ukuze kuboniswe ukuhlukahluka kweentlobo kwinkqubo nganye.Emva kweentsuku ezingama-60 zokufukamela, zonke izalathisi ezibonakalisiweyo ziyafana, oko kukuthi, i-tebuconazole isenokubangela ukuncipha kobutyebi beentlobo kunye nokwahluka komhlaba.Nangona kunjalo, ngokongeza i-WZ-2, uluntu lwebhaktiriya yomhlaba luye lwafunyanwa ngokuyinxenye ngokobutyebi kunye nokwahluka.Umahluko omncinci wabonwa phakathi kwe-BC, i-BCS kunye ne-CK, ebonisa ukuba i-biochar kunye ne-biochar-immobilized WZ-2 inokubuyisela ngokufanelekileyo impilo yebhayoloji yomhlaba.
Indlela yeqela elingenabunzima beqela elineeNdlela ze-Arithmetic (UPGMA) isetyenziswe kolu phononongo ukubonisa iyantlukwano ye-beta phakathi kwamaqela.Njengoko kubonisiwe kulo mzobo ulandelayo, i-BC, i-BSC kunye ne-CK yabelana ngepatheni efanayo yokwakheka kwe-microbial xa kuthelekiswa neqela le-T kunye ne-S, ebonisa ngakumbi ukuba ukuqaliswa kwe-biochar kwi-tebuconazole-engcolisekileyo ye-bioremediation yomhlaba kunokunceda kakhulu ukubuyiswa koluntu lwe-microbial emhlabeni.
Umzobo.Ukuhlanganiswa kwe-UPGMA yoluntu lwebhaktheriya kwinqanaba le-phylum phantsi kwonyango olwahlukileyo
Isalathiso
Ilanga, uTong, et al."Ukuhambelana kwebhaktheriya kunye nokunyanzeliswa kwe-biochar kuphuculwe ukuthotywa kwe-tebuconazole, ukubunjwa kwe-microbiome yomhlaba kunye nokusebenza."Ijenali Yezinto Eziyingozi398 (2020): 122941.
Funda isicatshulwa esipheleleyo
Iindaba neembalasane ijolise ekwabelaneni ngamatyala aphumeleleyo amva nje kunye ne-Biomarker Technologies, ukubamba impumelelo yesayensi enoveli kunye nobuchule obubalaseleyo obusetyenzisiweyo ngexesha lokufunda.
Ixesha lokuposa: Jan-08-2022