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条形 ibhanile-03

Iindaba

T2T INDIBANO YEGENOME, GAP FREE GENOME

1stIiGenome ezimbini zeRayisi1

Isihloko: INdibano kunye nokuQinisekiswa kweeGenomes ezimbini zeReferensi eziNgenaMpahla zeXian/indica Rice ityhila iiMfihlo kwiPlant Centromere Architecture

I-Doi:https://doi.org/10.1101/2020.12.24.424073

Ixesha elimiselweyo: NgoJanuwari 01, 2021.

Iziko: Huazhong Agricultural University, China

Izinto eziphathekayo

O. sativa xian/indicaiintlobo zerayisi 'Zhenshan 97 (ZS97)' kunye 'Minghui 63 (MH63)

Isicwangciso sokulandelelana

I-NGS iyafunda + i-HiFi ifundeka + i-CLR ifundeka + i-BioNano + i-Hi-C

Idatha:

I-ZS97: 8.34 Gb (~23x) i-HiFi ifunda + 48.39 Gb (~131x ) i-CLR ifundeka + 25 Gb (~69x) NGS + 2 iiseli ze-BioNano Irys

MH63: 37.88 Gb (~103x) i-HiFi ifundeka + 48.97 Gb (~132x) i-CLR ifundeka + 28 Gb(~76x) NGS + 2 iiseli zeBioNano Irys

Umfanekiso-1

Umzobo 1 Iigenomes ezimbini zerayisi (MH63 kunye neZS97)

2ndBanana Genome2

Isihloko: I-Telomere-to-telomere gapless chromosomes yebhanana isebenzisa ulandelelwano lwe-nanopore

I-Doi:https://doi.org/10.1101/2021.04.16.440017

Ixesha elibekiweyo: ngo-Epreli 17, 2021.

Iziko: IYunivesithi yaseParis-Saclay, eFransi

Izinto eziphathekayo

I-haploid ephindwe kabiniMusa acuminatasppisifo somhlaza(DH-Pahang)

Isicwangciso solandelelwano kunye nedatha:

Imowudi yeHiSeq2500 PE250 + MinION/ PromethION (93Gb, ~200X )+ Imephu yokubona (DLE-1+BspQ1)

Itheyibhile 1 Ukuthelekiswa kweendibano zegenome zikaMusa acuminata (DH-Pahang).

Itheyibhile1-Uthelekiso-lwe-GRCh38-kunye-T2T-CHM13-iindibano zegenome yabantu
Umfanekiso-Musa-genomes-architecture-comparison

Umzobo 2 Musa genomes uthelekiso lwezakhiwo

3rdI-Phaeodactylum tricornutum genome3

Isihloko: I-Telomere-to-telomere genome assembly ofP
haeodactylum tricornutum

I-Doi:https://doi.org/10.1101/2021.05.04.442596

Ixesha elibekiweyo: ngoMeyi 04, 2021

Iziko: IYunivesithi yaseNtshona, eCanada

Izinto eziphathekayo

Phaeodactylum tricornutum(Ukuqokelelwa kweNkcubeko yeAlgae kunye neProtozoa CCAP 1055/1)

Isicwangciso solandelelwano kunye nedatha:

I-1 Oxford Nanopore mini flow flow cell + i-2 × 75 idityanisiwe-yesiphelo phakathi kwemveliso NextSeq 550 run

Umfanekiso-Workflow-for-telomere-to-telomere-genome-assembly-1-1024x740

Umzobo 3 Ukuhamba komsebenzi kwindibano yegenome ye-telomere-to-telomere

4thI-CHM13 yegenome yomntu4

Isihloko: Ulandelelwano olupheleleyo lwegenome yomntu

I-Doi:https://doi.org/10.1101/2021.05.26.445798

Ixesha elibekiweyo: ngoMeyi 27, 2021

Iziko: Iziko leSizwe lezeMpilo (NIH), eU.SA

Izinto eziphathekayo: umgca weseli CHM13

Isicwangciso solandelelwano kunye nedatha:

30 × PacBio ulandelelwano lwesetyhula setyhula (HiFi) , 120 × Oxford Nanopore ultra-long read sequencing , 100 × Illumina PCR-Free sequencing (ILMN) , 70 × Illumina / Arima Genomics Hi-C (Hi-C), i-Bionos kunye neStrand-seq

Itheyibhile yesi-2 Uthelekiso lwe-GRCh38 kunye ne-T2T-CHM13 yeendibano zegenome yabantu

Itheyibhile-Uthelekiso-kaMusa-acuminata-DH-Pahang-genome-assemblies

Isalathiso

1.USergey Nurk et al.Ulandelelwano olupheleleyo lwegenome yomntu.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798

2.Caroline Belser et al.I-telomere-to-telomere gapless chromosomes yebhanana isebenzisa i-nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017

3.Daniel J. Giguere et al.Indibano ye-Telomere-to-telomere genome ye-Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596

4.Jia-Ming Ingoma et al.INdibano kunye nokuQinisekiswa kweeGenomes ezimbini zeReferensi ye-Xian/indica iRice ityhila iiNgcaciso kwiPlant Centromere Architecture.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073


Ixesha lokuposa: Jan-06-2022

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