UKUVELA KWEGENOME
Uphononongo lothelekiso lwe-genome luqaqambisa ukwandiswa kwegenome ye-transposon-mediated kunye nolwakhiwo lokuzivelela kwe-3D genomic esongwayo kumqhaphu.
Nanopore ulandelelwano |Hi-C |Ukulandelelana kwePacBio |Ilumina |RNA-ulandelelwano |I-3 D yoyilo lwe-genome |Transposon |Ukuthelekisa i-genomics
Kolu phononongo, i-Biomarker Technologies ibonelele ngenkxaso yobugcisa kwi-Nanopore sequencing, i-Hi-C kunye nohlalutyo olufanelekileyo lwe-bioinformatic.
Abstract
Ulwandiso lwe-Transposable element (TE) luqatshelwe njengamandla okuqhuba elamla ubungakanani be-genome kunye nendaleko, kodwa iziphumo zokubumba uyilo lwe-3D genomic zihlala zingaziwa kwizityalo.Apha, sinikela ingxelo yeendibano zegenome zenqanaba lereferensi kwiindidi ezintathu zomqhaphu eziphindwe kathathu kubungakanani begenome, ezizezi.Gossypium rotundifolium(K2),G. arboreum(A2), kunyeG. raimondii(D5), usebenzisa iOxford Nanopore Technologies.I-genome ethelekisayo ihlalutya uxwebhu lweenkcukacha zomnombo we-TE wokukhulisa ngokuthe ngqo onegalelo kumahluko omkhulu wesayizi ye-genome (K2, 2.44 Gb; A2, 1.62 Gb; D5, 750.19 Mb), kwaye ibonise umxholo wemfuza ogcinwe ngokwentelekiso kunye nobudlelwane be-synteny phakathi kweegenomes.Sifumanise ukuba malunga ne-17% yezakhi zofuzo ze-syntenic zibonisa utshintsho lwesimo se-chromatin phakathi kweendawo ezisebenzayo ("A") kunye nezingasebenziyo ("B"), kwaye ulwandiso lwe-TE lunxulunyaniswa nokwandiswa komyinge we-A compartment kwimimandla yemfuza (~ 7,000 genes. ) kwi-K2 kunye ne-A2 ngokunxulumene ne-D5.Kuphela yi-42% yemida ye-topologically associating domain (TAD) egciniweyo phakathi kweegenomes ezintathu.Idatha yethu ibandakanya ulwandiso lwakutsha nje lwee-TEs emva kokuyilwa kwemida ye-TAD engqalileyo.Olu pho nonongo lunika ukukhanya kwindima yokwandiswa kwegenome ye-transposon-mediated kwi-evolution ye-high-order chromatin structure kwizityalo.
Iinkcukacha-manani eziphambili zendibano ye-genome
Umzobo.Ukudityaniswa kweGenome kunye nenkcazo yesici se-G. rotundifolium (K2)
Iindaba neembalasane ijolise ekwabelaneni ngamatyala aphumeleleyo amva nje kunye neBiomarker Technologies, ukubamba impumelelo yesayensi enoveli kunye nobuchule obubalaseleyo obusetyenzisiweyo ngexesha lokufunda.
Ixesha lokuposa: Jan-05-2022