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Ulandelelwano lweMetagenomic (NGS)
I-Metagenome ibhekiselele kwingqokelela yemathiriyeli yemfuzo iyonke yoluntu oluxubeneyo lwezinto eziphilayo, ezifana ne-metagenome yokusingqongileyo, i-metagenome yabantu, njl njl.Ukulandelelana kwe-Metagenomic sisixhobo se-molecular esetyenziselwa ukuhlalutya izinto ezixubeneyo ze-genomic ezikhutshwe kwiisampuli zendalo, ezinikezela ngolwazi olucacileyo kwiintlobo zeentlobo zeentlobo kunye nobuninzi, ubume boluntu, ubudlelwane be-phylogenetic, izakhi zofuzo ezisebenzayo kunye nenethiwekhi yokulungelelanisa kunye nezinto ezisingqongileyo.
Iqonga:Illumina NovaSeq6000
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Ulandelelwano lweMetagenomic-Nanopore
I-Metagenomics sisixhobo se-molecular esetyenziselwa ukuhlalutya izinto ezixubileyo ze-genomic ezikhutshwe kwiisampuli zendalo, ezinikezela ngolwazi olucacileyo kwiintlobo ezahlukeneyo zeentlobo kunye nobuninzi, isakhiwo sabantu, ubudlelwane be-phylogenetic, izakhi zofuzo ezisebenzayo kunye nenethiwekhi yokulungelelanisa kunye nezinto ezisingqongileyo, njl njl. kwizifundo zemetagenomic.Ukusebenza kwayo okugqwesileyo kubude bokufunda kuphuculwe kakhulu uhlalutyo lwemetagenomic esezantsi, ngakumbi indibano yemetagenome.Ukuthatha izibonelelo zobude bokufunda, i-Nanopore-based based metagenomic study iyakwazi ukuphumeza indibano eqhubekayo ngokuthelekisa ne-shot-gun metagenomics.Kuye kwapapashwa ukuba i-metagenomics esekwe kwi-Nanopore yenze ngempumelelo i-genomes yebhaktiriya epheleleyo kunye nevaliweyo ukusuka kwi-microbiomes (Moss, EL, et. al.I-Biotech yendalo, 2020)
Iqonga:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Iyunithi ekwi-16S kunye ne-18S rRNA equlethe yomibini imimandla egcinwe kakhulu kunye ne-hyper-variable printing yeminwe egqibeleleyo yemolekyuli yokuchongwa kwe-prokaryotic kunye ne-eukaryotic organisms.Ukuthatha ithuba lolandelelwano, ezi amplicon zinokujoliswa ngokusekwe kumacandelo agciniweyo kwaye imimandla eguquguqukayo ephezulu inokuphawulwa ngokupheleleyo ngokuchongwa kwemicrobial enegalelo kwizifundo ezibandakanya uhlalutyo lwentlupheko yemicrobial, taxonomy, phylogeny, njl njl. ) ulandelelwano lweqonga lePacBio lwenza ukuba kufumaneke ufundo olude oluchanekileyo, olunokugubungela i-amplicon yobude obupheleleyo (malunga ne-1.5 KB).Umbono obanzi wendima yemfuza yasomeleza kakhulu isisombululo kwinkcazelo yeentlobo kwiibhaktiriya okanye kuluntu lomngundo.
Iqonga:I-PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
I-16S/18S/ITS i-amplicon sequencing ijolise ekuvezeni i-phylogeny, taxonomy, kunye nobuninzi beentlobo kwi-microbial community ngokuphanda iimveliso ze-PCR zeempawu zofuzo zokugcina izindlu eziqulethe zombini iindawo ezixoxwa kakhulu kunye ne-hypervariable.Ukwaziswa kwezi minwe zigqibeleleyo zemolekyuli nguWoeses et al, (1977) ixhobisa iprofayili ye-microbiome ezimeleyo.Ulandelelwano lwe-16S (iibhaktheriya), i-18S (umngundo) kunye ne-Internal transcribed spacer(ITS, ukungunda) ivumela ukuchongwa kweentlobo zezilwanyana ezininzi kunye neentlobo ezinqabileyo nezingaziwa.Le teknoloji iye yaba sisixhobo esisetyenziswa ngokubanzi kunye nesiphambili ekuchongeni ukubunjwa kwe-microbial eyahlukileyo kwiindawo ezahlukeneyo, ezinjengomlomo womntu, amathumbu, ilindle, njl.
Iqonga:Illumina NovaSeq6000
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IBacterial kunye neFungal Whole Genome Re-sequencing
Ibhaktiriya kunye nomngundo ulandelelwano ngokutsha lwegenome sisixhobo esibalulekileyo sokugqiba iigenomes zebhaktiriya eyaziwayo kunye nomngundo, kunye nokuthelekisa iigenomes ezininzi okanye ukwenza imephu yejenomi yezinto ezitsha.Kubaluleke kakhulu ukulandelelanisa iigenomes zonke zebhaktiriya kunye nomngundo ukuze kuveliswe iigenome zesalathiso ezichanekileyo, ukuchongwa kwe-microbial kunye nolunye uphononongo lothelekiso lwe-genome.
Iqonga: Illumina NovaSeq 6000
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I-Fungal Genome
I-Biomarker Technologies ibonelela ngesaveyi ye-genome, i-genome entle kunye ne-pene-complete genome ye-fungal ngokuxhomekeke kwinjongo ethile yophando.Ukulandelelana kweGenome, indibano kunye nenkcazo esebenzayo inokufezekiswa ngokudibanisa isizukulwana esilandelayo + Ukulandelelana kwesizukulwana sesithathu ukuphumeza indibano yegenome yenqanaba eliphezulu.Itekhnoloji ye-Hi-C inokuqeshwa ukuququzelela ukudibanisa i-genome kwinqanaba le-chromosome.
Iqonga:I-PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq 6000
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Iintsholongwane ziGqibezela iGenome
I-Biomarker Technologies ibonelela ngenkonzo yolandelelwano ekwakheni i-genome epheleleyo yebhaktiriya ene-gap engu-zero.Ukuhamba komsebenzi okuphambili kwebhaktheriya ukwakhiwa kwegenome okupheleleyo kubandakanya ulandelelwano lwesizukulwana sesithathu, ukudibanisa, inkcazo esebenzayo kunye nohlalutyo oluphambili lwe-bioinformatic oluzalisekisa iinjongo ezithile zophando.Iprofayili ebanzi ngakumbi yeebhaktheriya genome ixhobisa ukutyhilwa kweendlela ezisisiseko ezisisiseko senkqubo yazo yebhayoloji, enokubonelela ngereferensi ebalulekileyo kuphando lwegenomic kwiintlobo eziphezulu ze-eukaryotic.
Iqonga:Nanopore PromethION P48 + Illumina NovaSeq 6000
I-PacBio Sequel II
