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Iimveliso

Ulandelelwano lweMetatranscriptome

Ulandelelwano lweMetatranscriptome Umfanekiso obonakalayo
  • Ulandelelwano lweMetatranscriptome

Ulandelelwano lweMetatranscriptome luchonga ukubonakaliswa kofuzo lwemicrobes (zombini ieukaryotes kunye neprokaryotes) ngaphakathi kokusingqongileyo kwendalo (oko kukuthi, umhlaba, amanzi, ulwandle, ilindle, kunye namathumbu.) Ngokukodwa, ezi nkonzo zikuvumela ukuba ufumane iprofayili yegenesis epheleleyo yeentsholongwane ezintsonkothileyo, uhlalutyo lwetaxonomic. kweentlobo, uhlalutyo lokutyebisa olusebenzayo lwemfuza echazwe ngokwahlukileyo, kunye nokunye.

Iqonga: I-Illumina NovaSeq Platform


Iinkcukacha zeNkonzo

IiMfuno zeSampulu

Iintlobo zeesampuli: Iyonke i-RNA;

Isixa: 2.5ug;

Ugxininiso: 50ng/ul;

IQhinga leeNkonzo

Uhlobo lweThala leencwadi: 250-300bp faka ilayibrari ye-cDNA;

I-Platform yokulandelelana kunye neSicwangciso: i-illumina NovaSeq Platform; i-paired-end 150bp;

IsiPhumo seDatha esiCetyisiweyo: 12G idatha ekrwada/isampulu

Ukuhamba komsebenzi weNkonzo

ukuhanjiswa kwesampuli

Ukuhanjiswa kwesampuli

ULungiselelo lweThala leeNcwadi

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Ukulandelelanisa

Uhlalutyo lwedatha

Uhlalutyo lwedatha

Emva kweeNkonzo zentengiso

Iinkonzo emva kokuthengisa

  • Ngaphambili:
  • Okulandelayo:

    • fumana isicatshulwa

    Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    Imvelisoiindidi

    Thumela umyalezo wakho kuthi:

    Cofa u-enter ukukhangela okanye i-ESC ukuvala