Iimveliso

Uhlalutyo lwe-Genome-wide Association

Genome-wide Association Uhlahlelo Featured Image

I-Genome-wide association study (GWAS) ijolise ekuchongeni ukuhlukahluka kofuzo (i-genotype) ehambelana neempawu ezithile (phenotype).Uphononongo lwe-GWAS luphanda iimpawu zofuzo ezinqamlezileyo kwi-genome yenani elikhulu labantu kwaye liqikelela imibutho ye-genotype-phenotype ngohlalutyo lwamanani kwinqanaba labantu.Isetyenziswe ngokubanzi kuphando ngezifo zabantu kunye nokwembiwa kwemfuza okusebenzayo kwiimpawu ezinzima zezilwanyana okanye izityalo.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Iziphumo zeDemo

Ushicilelo olukhoyo

1.Iinzuzo zeNkonzo

● Iimeko zeProjekthi ezininzi: ukususela ekusekweni kwayo ngo-2009, i-BMKGENE igqibe amakhulu eeprojekthi zeentlobo kuphando lwabantu be-GWAS, incedise abaphandi ukuba bapapashe amanqaku angaphezu kwe-100, kwaye i-cumulative factor factor ifike kwi-500.
● Abahlalutyi beengcali.
● Umjikelo omfutshane wohlalutyo.
● Ukumbiwa kwedatha ngokuchanekileyo.

2. IiNgcaciso zeNkonzo

Uhlobo	Umlinganiselo Wabemi	Isicwangciso solandelelwano nobunzulu
I-SLAF-GWAS	Inombolo yesampuli ≥200	Ubungakanani beGenome <400M, kunye ne-ref-genome, i-WGS iyacetyiswa
		Ubungakanani beGenome ≤ 1G, 100K iithegi kunye ne-10X
		1G ≤ Ubungakanani beGenome ≤ 2G, 200K iithegi kunye ne-10X
		Ubungakanani beGenome> 2G, 300K iithegi kunye ne-10X
I-WGS-GWAS	Inombolo yesampuli ≥200	10X kwisampulu nganye

3. Ukukhethwa kwezinto

Iindidi ezahlukeneyo, i-subspecies, i-landraces / i-genebanks / iintsapho ezixutywe / izixhobo zasendle

Iintlobo ezahlukeneyo, i-subspecies, i-landraces

Usapho lwesiqingatha sosapho / usapho olugcweleyo / izixhobo zasendle

4. Uhlalutyo lwe-Bioinformation

● Uhlalutyo lonxulumano olubanzi lweGenome
● Uhlalutyo kunye nokuhlolwa kwe-SNP ebalulekileyo
● Inkcazo esebenzayo yejini yomviwa

5. UkuHamba komsebenzi weNkonzo

Uyilo lovavanyo

Ukuhanjiswa kwesampuli

Ukukhutshwa kwe-RNA

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Uhlalutyo lwedatha

Iinkonzo emva kokuthengisa

Ngaphambili: I-nucleus ye-RNA enye

Okulandelayo:

a.Phenotype QC

Ihistogram yosasazo rhoqo

Izibalo zePhenotype

b.Uhlalutyo lombutho (Umzekelo: GEMMA, Fast-LMM, EMMAX)

Iploti yeQQ

Iploti yaseManhattan

Unyaka	Ijenali	IF	Isihloko
2022	NC	17.69	Isiseko seGenomic segiga-chromosomes kunye negiga-genome yomthi wepeony Paeonia ostii
2015	NP	7.43	Imizila yasekhaya ixhasa imimandla ye-genomic yokubaluleka kwe-agronomic kwiimbotyi zesoya
2018	MP	9.32	Ukulandelelana kwegenome ngokupheleleyo kwingqokelela yehlabathi jikelele ye-rapeseed accessions ityhila isiseko sofuzo sokwahlukana kwe-ecotype yabo
2022	HR	7.29	Uhlalutyo lwe-genome-wide-wide association lubonelela ngengqiqo yeemolekyuli kwinguqu yendalo yobungakanani bembewu yevatala