Uhlalutyo lwe-Eukaryotic mRNA (ngokubhekiselele)
Ngokusekelwe kulandelelwano lwe-genome olwaziwayo kunye nolwazi lwe-annotation, isizukulwana esitsha se-high throughput sequencing (RNA-Seq) idatha isetyenziswa njengegalelo, kunye neendawo ezintsha zokukhuphela (i-gene entsha) kunye neziganeko ezintsha zokudibanisa eziguquguqukayo zichongiwe.ukulandelelanisa uvavanyo lomgangatho wedatha;idatha yokulandelelanisa kunye nolandelelwano lolandelelwano lwe-genome yereferensi ekhethiweyo, ukuchonga imida ye-exon / intron, ukuhlalutya i-gene variant splicing, ukuhlola imimandla yegene kunye nemibhalo emitsha, ukuchonga iindawo ze-SNP zommandla obhaliweyo, umda phakathi kwe-3' kunye ne-5 iigenes, kunye nenkcazo esebenzayo kunye nohlalutyo lokutyebisa lweesampulu ezahlukeneyo (amaqela).
Ii-RNA ezinde ezingabhaliyo
Ii-RNA ezinde ezingezizo iikhowudi (lncRNA) luhlobo lwemibhalo enobude obude kune-200 nt, engakwaziyo ukufaka iiprotheyini zekhowudi.Ubungqina obuninzi bucebisa ukuba uninzi lwee-lncRNAs kusenokwenzeka ukuba zisebenze.Itekhnoloji yokulandelelaniswa komgangatho ophezulu kunye nezixhobo zokuhlalutya i-bioinformatic zisixhobisa ukuba siveze ulandelelwano lwe-lncRNA kunye nokubeka ulwazi ngokufanelekileyo kwaye sikhokelele ekufumaneni ii-lncRNAs ezinemisebenzi ebalulekileyo yokulawula.I-BMKCloud inebhongo ngokubonelela abathengi bethu ngeqonga lokuhlalutya lolandelelwano lwe-lncRNA ukuphumeza uhlalutyo olukhawulezayo, oluthembekileyo noluguquguqukayo lwe-lncRNA.
16S/18S/ITS ulandelelwano lweamplicon
Iqonga lokuhlalutya lokwahluka kweMicrobial liphuhliswa ngeminyaka yamava kuhlalutyo lweprojekthi yokwahlukana kwemicrobial, equlathe uhlalutyo olusisiseko olusemgangathweni kunye nohlalutyo lomntu siqu: uhlalutyo olusisiseko lugubungela umxholo wohlalutyo oluphambili lophando lwangoku lwemicrobial, umxholo wohlalutyo utyebile kwaye ubanzi, kwaye iziphumo zohlalutyo zinikezelwe. ngokohlobo lweengxelo zeprojekthi;Umxholo wohlalutyo lobuqu uhlukile.Iisampulu zinokukhethwa kwaye iiparameters zinokumiselwa ngokuguquguqukayo ngokwengxelo yohlalutyo olusisiseko kunye nenjongo yophando, ukufezekisa iimfuno zomntu.Inkqubo yokusebenza yeWindows, ilula kwaye iyakhawuleza.
Umpu Metagenomics(NGS)
Idatha ye-Metagenomic isetyenziselwa ukuhlalutya izinto ezixubileyo ze-genomic ezikhutshwe kwiisampuli zendalo, ezinikezela ngolwazi olucacileyo malunga nokuhlukahluka kweentlobo kunye nobuninzi, isakhiwo sabantu, ubudlelwane be-phylogenetic, izakhi zofuzo ezisebenzayo, kunye nenethiwekhi yokulungelelanisa kunye nezinto ezisingqongileyo.
I-NGS-WGS(Illumina/BGI)
Umbhobho wokuhlalutya odibeneyo ophuhliswe kwiingcali zophando ngaphandle kolwazi lwangaphambili lwe-bioinformatics kwaye uqhuba kwi-server ephezulu yokusebenza.Iququzelela ukugqitywa okukhawulezileyo kwemisebenzi efana nolawulo lomgangatho wedatha, ulungelelwaniso lolandelelwano, ukubhaqwa kokwahluka kwe-SNP/InDel/SV, isichasiselo, kunye negene yokuguqula.
I-GWAS
Ngokusebenzisa iindlela zobalo ezithile, uhlalutyo lwe-GWAS lujolise ekutyhileni i-genome-wide nucleotide variations ehambelana ne-phenotypic differences.Idlala indima ebalulekileyo ekuphononongeni imizila yemfuza esebenzayo eyayanyaniswa nezifo ezintsonkothileyo zabantu kunye neempawu ezintsonkothileyo kwizityalo nakwizilwanyana.
BSA
Iqonga lokuhlalutya elihlanganisiweyo libonelela ngojongano olusebenzisekayo olulungelelanisiweyo kunye nohlalutyo lweyantlukwano yobuqu.Uhlalutyo lwe-BSA lubandakanya ukudibanisa abantu abaneempawu zephenotypic ezigqithisileyo ukusuka kuluntu olwahlulayo.Ngokuthelekisa i-loci yokwahlula phakathi kweesampulu ezidityanisiweyo, le ndlela ichonga ngokukhawuleza iziphawuli zeemolekyuli ezinxulunyaniswa nofuzo ekujoliswe kulo.Isetyenziswa kakhulu kwimaphu yofuzo yezityalo nezilwanyana, sisixhobo esixabisekileyo sokumakisha-uncedo lokuzala kunye nokubekwa kwemfuza.
I-Evolutionary Genetics
Luhambo oludityanisiweyo lohlalutyo olulungiselelwe iingcali zophando ezinobuchwephesha obulinganiselweyo be-bioinformatics.Ukuxhasa amava abanzi e-BMKGENE kwiiprojekthi zokuzivelela kofuzo, eli qonga lisebenza kwiiseva ezisebenza kakhulu, liqinisekisa ukwenziwa kohlalutyo oluchanekileyo kunye noluchanekileyo.Le misebenzi ibandakanya ulwakhiwo lwemithi ye-phylogenetic, uhlalutyo lokungalungelelani konxibelelwano, uvavanyo lweyantlukwano yemfuza, ukuchongwa kokutshayela okukhethiweyo, uhlalutyo lokuzalana, uhlalutyo lwecandelo eliphambili, kunye nokubonakaliswa kobume boluntu.
